Cowpea is a very popular foodstuff among people in sub-Saharan Africa. In Burkina Faso, it is the main food legume, especially in rural areas. Its production is facing difficulties including post-harvest losses caused by fungi. Therefore, the objective of this study was to isolate and identify fungal strains associated with cowpea seeds produced in Burkina Faso. Thus, a total of 108 seed samples were collected in the three agro-ecological zones of Burkina Faso. The sanitary analysis of the seeds was carried out using the direct contact method. The isolation and purification of the isolates were performed on Potato Dextrose Agar medium while their identification was done through macroscopic and microscopic phenotypical characterization using different culture media (Potato Dextrose Agar (PDA), Malt Extract Agar (MEA) and Czapeck Dox Agar (CZA)) and different identification keys. A total of 10 fungal species were isolated, with predominance of Aspergillus flavus, Aspegillus niger, Macrophomina phaseolina, Fusarium oxysporium and Rhizopus sp. whose infection rates were 70.8% to 100% of seed samples. In addition to being present in all three zones, the infection rates of Aspergillus flavus (56.55%), Aspergillus niger (20.35%) and Rhizopus (32.80%) were higher in the Sahelian zone. In the Sudano-Sahelian zone, Macrophomina (50.66%) and Fusarium (18.88%) presented the highest infection rates, while Penicillium sp. showed the highest infection rate (2.84%) in the Sudanian zone. This finding demonstrated the necessity to improve post-harvest and conservation techniques of cowpea to limit crop losses and preserve the sanitary quality of this important foodstuff.
Ngamo, L.S.T. and Hance, T. (2007) Diversité des ravageurs des denrées et méthodes alternatives de lutte en milieu tropical. Tropicultura, 25, 215-220.
Haro, H., Sanon, K.B., Le Roux, C., Duponnois, R. and Traoré, A.S. (2018) Improvement of Cowpea Productivity by Rhizobial and Mycorrhizal Inoculation in Burkina Faso. Symbiosis, 74, 107-120. https://doi.org/10.1007/s13199-017-0478-3
Njoroge, A.W., Baoua, I. and Baributsa, D. (2019) Postharvest Management Practices of Grains in the Eastern Region of Kenya. Journal of Agricultural Science, 11, 33-42. https://doi.org/10.5539/jas.v11n3p33
Williams, S.B., Baributsa, D. and Woloshuk, C. (2014) Assessing Purdue Improved Crop Storage (PICS) Bags to Mitigate Fungal Growth and Aflatoxin Contamination. Journal of Stored Products Research, 59, 190-196. https://doi.org/10.1016/j.jspr.2014.08.003
Kpoviessi, A.D., Agbahoungba, S., Agoyi, E.E., Nuwamanya, E., Assogbadjo, A.E., Chougourou, D.C. and Adoukonou-Sagbadja, H. (2021) Primary and Secondary Metabolite Compounds in Cowpea Seeds Resistant to the Cowpea Bruchid [Callosobruchus maculatus (F.)] in Postharvest Storage. Journal of Stored Products Research, 93, Article 101858. https://doi.org/10.1016/j.jspr.2021.101858
FAO (2010) Reducing Post-Harvest Losses in Grain Supply Chains in Africa: Lessons Learned and Practical Guidelines. Italy.
Sheahan, M. and Barrett, C.B. (2017) Food Loss and Waste in Sub-Saharan Africa: A Critical Review. Food Policy, 70, 1-12. https://doi.org/10.1016/j.foodpol.2017.03.012
Kabir, B.G.J., Audu, A. and Bukar, F.M.G.B.B. (2017) Evaluation of Cassia sieberiana (DC) and Vernonia amygdalina (Del.) against Callosobruchus maculatus (F.) Infesting Stored Bambara Groundnut (Vigna subterranea (L.) Verdc.). Tropical and Subtropical Agroecosystems, 20, 223-230.
Holban, A.M. and Grumezescu, A.M. (2017) Handbook of Food Bioengineering. Foods Therapeutic, 8, 538.
Makun, H.A., Anjorin, S.T., Abidoye, A.S., Rufai, A.R. and Kabiru, Y.A. (2012) Incidence and Botanical Control of Seed-Borne Fungi of Cowpea in Niger State, Nigeria. ARPN Journal of Agricultural and Biological Science, 7, 654-658.
Gyasi, E., Kotey, D.A., Adongo, B.A., Adams, F.K., Owusu, E.O. and Mohammed, A. (2022) Management of Major Seed-Borne Fungi of Cowpea (Vigna unguiculata (L.) Walp) with Four Selected Botanical Extracts. Advances in Agriculture, 2022, Article ID: 3125240. https://doi.org/10.1155/2022/3125240
Khare, K.B., Loeto, D., Wale, K. and Salani, M. (2016) Seed-Borne Fungi of Cowpea [Vigna unguiculata (L.) Walp] and Their Possible Control in Vitro Using Locally Available Fungicides in Botswana. International Journal of Bioassays, 5, 5021-5024. https://doi.org/10.21746/ijbio.2016.11.005
Perrone, G., Susca, A., Cozzi, G., Ehrlich, K., Varga, J., Frisvad, J.C., Meijer, M., Noonim, P., Mahakarnchanakul, W. and Samson, R.A. (2007) Biodiversity of Aspergillus Species in Some Important Agricultural Products. Studies in Mycology, 59, 53-66. https://doi.org/10.3114/sim.2007.59.07
Marasas, W.F.O., Jaskiewicz, K., Venter, F.S. and Van Schalkwyk, D.J. (1988) Fusarium moniliforme Contamination of Maize in Oesophageal Cancer Areas in Transkei. The South African Medical Journal, 74, 110-114.
Klich, M. (2002) Identification of Common Aspergillus Species. Centraalbureau voor Schimmelcultures, Utrecht.
Samson, R.A., Houbraken, J., Thrane, U., Frisvard, J.C. and Andersen, B. (2010) Food and Indoor Fungi. Centraalbureau voor Schimmelcultures, Utrecht.
Samson, R.A., Noonim, P., Meijer, M., Houbraken, J., Frisvad, J.C. and Varga, J. (2007) Diagnostic Tools to Identify Black Aspergilli. Studies in Mycology, 59, 129-145. https://doi.org/10.3114/sim.2007.59.13
Samson, R.A., Hong, S., Peterson, S.W., Frisvad, J.C. and Varga, J. (2007) Polyphasic Taxonomy of Aspergillus Section Fumigati and Its Teleomorph Neosartorya. Studies in Mycology, 59, 147-203. https://doi.org/10.3114/sim.2007.59.14
Afolabi, C.G., Ezekiel, C.N., Ogunbiyi, A.E., Oluwadairo, O.J., Sulyok, M. and Krska, R. (2019) Fungi and Mycotoxins in Cowpea (Vigna unguiculata L) on Nigerian Markets. Food Additives & Contaminants: Part B, 13, 52-58. https://doi.org/10.1080/19393210.2019.1690590
Zanjare, S., Balgude, Y., Zanjare, S.S., Suryawanshi, A. and Shelar, V. (2020) Detection of Seed Borne Myco-Flora Associated with Cowpea (Vigna unguiculata L. Walp). International Journal of Chemical Studies, 8, 1585-1587. https://doi.org/10.22271/chemi.2020.v8.i1w.8482
Degraeve, S., Madege, R.R., Audenaert, K., Kamala, A., Ortiz, J., Kimanya, M., Tiisekwa, B., De Meulenaer, B. and Haesaert, G. (2016) Impact of Local Pre-Harvest Management Practices in Maize on the Occurrence of Fusarium Species and Associated Mycotoxins in Two Agro-Ecosystems in Tanzania. Food Control, 59, 225-233. https://doi.org/10.1016/j.foodcont.2015.05.028
Baddi, M., Nassik, S., Alali, S. and El Hraiki, A. (2021) L’impact économique et sanitaire des mycotoxines entre aujourd’hui et demain. Revue Marocaine des Sciences Agronomiques et Vétérinaires, 9, 339-347.
Abdallah, M.F., Girgin, G. and Baydar, T. (2019) Mycotoxin Detection in Maize, Commercial Feed, and Raw Dairy Milk Samples from Assiut City, Egypt. Veterinary Sciences, 6, Article 57. https://doi.org/10.3390/vetsci6020057
Okayo, R.O., Andika, D.O., Dida, M.M., K’otuto, G.O. and Gichimu, B.M. (2020) Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya. International Journal of Microbiology, 2020, Article No. 8854718. https://doi.org/10.1155/2020/8854718
Ono, L.T., Silva, J.J., Doná, S., Martins, L.M., Iamanaka, B.T., Fungaro ,M.H.P., Pitt, J.I. and Taniwaki, M.H. (2021) Aspergillus Section Flavi and Aflatoxins in Brazilian Cassava (Manihot esculenta Crantz) and Products. Mycotoxin Research, 37, 221-228. https://doi.org/10.1007/s12550-021-00430-2
Imane, K., and Mouhamed, S. (2012) Isolement de quelques champignons qui polluent l’eau d’irrigation de la faculté d’agriculture de l’Université de Baghdad. Revue iraquienne de la science agronomique, 43, 76-84.
Hedayati, M.T., Pasqualotto, A.C., Warn, P.A., Bowyer, P. and Denning, D.W. (2007) Aspergillus flavus: Human Pathogen, Allergen and Mycotoxin Producer. Microbiology, 153, 1677-1692. https://doi.org/10.1099/mic.0.2007/007641-0
Shahnaz, D., Maimona, K. and Summiaya, R. (2015) Seed Borne Fungi Associated with Cowpea (Vigna unguiculata (L.) Walp. International Journal of Biology and Biotechnology, 12, 565-569.
Ouili, S.A., Maiga, Y., Zida, P.E., Adjima, O., Nankangre, H., Compaoré, C.O.T., Nikiéma, M., Ouédraogo, M. and Ouattara, A.S. (2022) Isolation and Characterization of Fungal Strains from the Seeds of Bambara Groundnut (Vigna subterranea (L.) Verdcourt) Produced in Burkina Faso. African Journal of Food Science, 16, 107-115. https://doi.org/10.5897/AJFS2022.2168
Nikiema, F.W., Zida, E.P., Thio, G.I., Nitiéma, L.W., Koita, K. and Sawadogo, M. (2020) Incidence de Fusarium spp. associé aux semences de riz (Oryza sativa L.) au Burkina Faso. International Journal of Biological and Chemical Sciences, 14, 2160-2171. https://doi.org/10.4314/ijbcs.v14i6.18
Bouajila, A., Lamine, M., Rahali, F.Z., Melki, I., Prakash, G. and Ghorbel, A. (2020) Pearl Millet Populations Characterized by Fusarium Prevalence, Morphological Traits, Phenolic Content, and Antioxidant Potential. Journal of the Science of Food and Agriculture, 100, 4172-4181. https://doi.org/10.1002/jsfa.10456
Pfohl-Leszkowicz, A. (1999) Les mycotoxines dans l’alimentation: évaluation et gestion du risque. Technique et documentation-Lavoisier, 478.
Omoigui, L.O., Kamara, A.Y., Batieno, J., Iorlamen, T., Kouyate, Z., Yirzagla, J., Diallo, S. and Garba, U. (2018) Guide sur la production du niébé en Afrique de l’Ouest Transforming African Agriculture. Institut international d’agriculture tropicale, Ibadan.
Aoki, T., O’Donnell, K. and Geiser, D.M. (2014) Systematics of Key Phytopathogenic Fusarium Species: Current Status and Future Challenges. Journal of General Plant Pathology, 80, 189-201. https://doi.org/10.1007/s10327-014-0509-3
Zida, E.P., Sérémé, P., Vibeke, L., Philipe, S., Irénée, S. and Adama, N. (2008) Importance of Seed Born Fungi of Sorghum and Pearl Millet in Burkina Faso and Their Control Using Plant Extracts. Pakistan Journal of Biological Sciences, 11, 321-331. https://doi.org/10.3923/pjbs.2008.321.331
Ouoba, A., Zida, S.F., Ouédraogo, M., Nandkangre, H., Ouédraogo, H.M., Nanéma, R.K., Sawadogo, N., Zida, E.P., Konaté, N.M., Congo, A.K., Soalla, R.W. and Sawadogo, M. (2017) Assessment of Genetic Diversity in Bambara Groundnut (Vigna subterranea (L.) Verdcourt) Landraces in Burkina Faso Using Microsatellite Markers (SSR). Agricultural Science Research Journal, 7, 96-102.
Kpatinvoh, B, Adjou, E.S., Dahouenon-Ahoussi, E., Konfo, T.R.C., Atrevi, B., Soumanou, M.M. and Sohounhloue, D.C.K. (2017) Efficacité des huiles essentielles de trois plantes aromatiques contre la mycoflore d’altération du niébé (Vigna unguiculata L., Walp) collecté dans les magasins de vente du Sud-Bénin. Journal of Applied Biosciences, 109, 10680-10687. https://doi.org/10.4314/jab.v109i1.12
Jyoshna, M. and Saxena, N. (2021) Isolation of Seed Mycoflora of Cowpea Seeds. International Journal of Aquatic Science, 12, 4728-4732.
Pitt, J.I. (2000) Toxigenic Fungi: Which Are Important? Medical Mycology, 38, 17-22. https://doi.org/10.1080/mmy.38.s1.17.22
Thies, J.A., Berland, P.A. and Fery, R.L. (2019) Response of Cowpea Cultivars to Rhizoctonia solani in Field Tests at Four Planting Dates. HortScience, 41, 497-520. https://doi.org/10.21273/HORTSCI.41.3.516A
El-Dawy, E.G.A.E.M., Gherbawy, Y.A. and Hussein, M.A. (2021) Morphological, Molecular Characterization, Plant Pathogenicity and Biocontrol of Cladosporium Complex Groups Associated with Faba Beans. Scientific Reports, 11, Article No. 14183. https://doi.org/10.1038/s41598-021-93123-w
Pitt, J.I. and Hocking, A.D. (1997) Fungi and Food Spoilage. 2nd Edition, Springer, New York. https://doi.org/10.1007/978-1-4615-6391-4