Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous Roots Extracts of <i>Carica papaya</i> L. and <i>Cocos nucifera</i> L. — Oak Academic Publishing
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Anti-Biofilm, Anti-Quorum Sensing, and Anti-Proliferative Activities of Methanolic and Aqueous Roots Extracts of <i>Carica papaya</i> L. and <i>Cocos nucifera</i> L.
Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
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Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
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Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
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Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
,
Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
,
Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
,
Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
,
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
,
Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
,
International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso
1 Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
2 Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
3 Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
4 Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
5 Laboratory of Molecular Biology and Genetics (LABIOGENE), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
6 Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
7 Department of Traditional Medicine and Pharmacopoeia-Pharmacy, Health Sciences Research Institute, Ouagadougou, Burkina Faso
8 Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
9 Laboratory of Biochemistry and Applied Chemistry (LABIOCA), Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou, Burkina Faso
10 International Research Laboratory: Environnement, Santé, Sociétés (IRL 3189, ESS) CNRST/CNRS/UCAD/UGB/USTTB, Ouagadougou, Burkina Faso
Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli , Pseudomonas aeruginosa , Streptococcus mutans , and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum . Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC 50 = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC 50 = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.
KeywordsTriterpenes and Sterols ContentAntibacterialBiofilmQuorum SensingAnti-ProliferativeMedicinal Plants
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