Study on the Effect of Shuanghuanglian Powder Needle Combined with Cefoperazone and Sulbactam Sodium on <i>Pseudomonas aeruginosa in Vitro</i> — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Study on the Effect of Shuanghuanglian Powder Needle Combined with Cefoperazone and Sulbactam Sodium on <i>Pseudomonas aeruginosa in Vitro</i>
Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
,
Laboratory Department of the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China
,
Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
,
Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
,
Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
,
Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
,
Department of Pediatrics, Nanning Third People’s Hospital, Nanning, China
,
Laboratory Department of People’s Hospital of Guangxi ASEAN Economic and Technological Development Zone, Nanning, China
,
Laboratory Department of Nanning 10th People’s Hospital, Nanning, China
1 Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
2 Laboratory Department of the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China
3 Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
4 Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
5 Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
6 Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China
7 Department of Pediatrics, Nanning Third People’s Hospital, Nanning, China
8 Laboratory Department of People’s Hospital of Guangxi ASEAN Economic and Technological Development Zone, Nanning, China
9 Laboratory Department of Nanning 10th People’s Hospital, Nanning, China
Abstract Objective: To explore the antibacterial activity of combined use of Shuanghuanglian and cefoperazone sulbactam sodium on resistant strains of Pseudomonas aeruginosa . Methods: The Pseudomonas aeruginosa strains which were sensitive and resistant to cefoperazone sulbactam sodium were selected to prepare different test bacterial solutions respectively; The experimental liquid of Shuanghuanglian and Cefoperazone Sulbactam Sodium were prepared separately and set as different test groups and control groups; The Drug Sensitivity Tests of Shuanghuanglian and cefoperazone sulbactam sodium at different concentration gradients which were used alone or used in combination were carried out for different strains with sensitivity and resistance, And use standard entry as a reference control. Result: The results of drug sensitivity test of Shuanghuanglian combined with Cefoperazone-Sulbactam sodium against the resistant strains of Pseudomonas aeruginosa were compared with the results of drug sensitivity test of the two separately used, and the difference was statistically significant (P Pseudomonas aeruginosa resistant strains were statistically significant compared with the drug sensitivity test results of Shuanghuanglian and Cefoperazone Sulbactam Sodium used separately (P Conclusion: The combination of Shuanghuanglian and cefoperazone sulbactam sodium has synergistic antibacterial or bactericidal effect on Pseudomonas aeruginosa resistant strains.
KeywordsShuanghuanglianCefoperazone-Sulbactam SodiumUse in Combination<i>Pseudomonas aeruginosa</i>Drug ResistanceBacteriostasis
Mielko, K.A., Jabłoński, S.J., Milczewska, J., et al. (2019) Metabolomic Studies of Pseudomonas aeruginosa. World Journal of Microbiology and Biotechnology, 35, Article No. 178. https://doi.org/10.1007/s11274-019-2739-1
Xu, Y.F., Wang, H.M. and Zhang, D. (2019) Study on the Mechanism of Resistance of Pseudomonas aeruginosa to Carbapenem antibiotics. Journal of Baotou Medical College, 35, 108-112. https://doi.org/10.16833/j.cnki.jbmc.2019.09.048
Yu, Y., Shao, X.S., Yang, W.P., Dong, K.G. and Komg, M. (2018) Clinical Distribution and Drug Resistance of Pseudomonas aeruginosa in a Tertiary Hospital. Chinese Journal of Disinfection, 35, 63-66. (In Chinese)
Liu, Y., Wang, M.Q., Zhu, B.T., et al. (2019) Analysis of Drug Resistance and Homology of Pseudomonas aeruginosa in Packaged Drinking Water. China Brewing, 38, 132-135. (In Chinese)
Huang, X.M. and Tang, W.N. (2019) Distribution and Drug Resistance Analysis of Pseudomonas aeruginosa from 2015 to 2019. Electronic Journal of Clinical Medical Literature, 6, 180. (In Chinese)
The Expert Committee of Rational Drug Use of the National Health Commission and National Bacterial Drug Resistance Monitoring Network (2021) 2019 National Bacterial Drug Resistance Monitoring Report. Exploration of Rational Drug Use in China, 18, 1-11. (In Chinese)
Botelho, J., Grosso, F. and Peixe, L. (2019) Antibiotic Resistance in Pseudomonas aeruginosa—Mechanisms, Epidemiology and Evolution. Drug Resistance Updates, 44, Article ID: 100640. https://doi.org/10.1016/j.drup.2019.07.002
Chen, R.D. and Li, Y.J. (2018) Experimental Study on the in Vitro Antibacterial Effect of 10 Chinese Herbal Medicines on Common Clinical Pathogenic Bacteria. Anti infective Pharmacy, 15, 196-198. (In Chinese)
Huang, X.H., Gao, G.Y. and Huang, Z.A. (2018) In Vitro Bacteriostatic Effect of 12 Kinds of Traditional Chinese Medicines on 20 Strains of Extensively Drug-Resistant Pseudomonas aeruginosa. Chinese Practical Medicine, 13, 196-198. (In Chinese)
Zhou, X. and Zhou, B.J. (2017) In Vitro Antibacterial Activity of 113 Chinese Herbal Decoction against Pseudomonas aeruginosa. Chinese Medicine Forum, No. 4, 59-61. (In Chinese)
Jiang, W.J. and Liang, X. (2018) A Study on the Reversal of Bacterial Resistance and the Effect of Scutellaria baicalensis Extract on the Expression of 16S rRNA Methylase Gene. Modern Medicine and Health, 34, 3493-3496. (In Chinese)
Liu, C.L. and Wang, J.H. (2019) Research Progress on the Mechanism of Traditional Chinese Medicine against Drug-Resistant Pseudomonas aeruginosa. Chinese Medical Journal, 16, 40-43. (In Chinese)
Weinstein, M.P. and Lewis, J.S. (2020) Commentary: The Clinical and Laboratory Standards Institute (CLSI) Subcommittee on Antimicrobial Susceptibility Testing: Background, Organization, Functions, and Processes. Journal of Clinical Microbiology, 58, e01864-19. https://doi.org/10.1128/JCM.01864-19
Shang, H., Wang, Y.S. and Shen, Z.Y. (2015) National Operating Procedures for Clinical laboratory. People’s Health Publishing House, Beijing, 11-50. (In Chinese)
Humphries, R., Bobenchik, A.M., Hindler, J.A. and Schuetz, A.N. (2021) Overview of Changes to the Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, M100, 31st Edition. Journal of Clinical Microbiology, 59, e0021321. https://doi.org/10.1128/JCM.00213-21
Zhang, P., Guo, Q., Wei, Z., et al. (2021) Baicalin Represses Type Three Secretion System of Pseudomonas aeruginosa through PQS System. Molecules, 26, Article No. 1497. https://doi.org/10.3390/molecules26061497
Luo, J., Dong, B., Wang, K., et al. (2017) Baicalin Inhibits Biofilm Formation, Attenuates the Quorum Sensing-Controlled Virulence and Enhances Pseudomonas aeruginosa Clearance in a Mouse Peritoneal Implant Infection Model. PLOS ONE, 12, e0176883. https://doi.org/10.1371/journal.pone.0176883
Dong, B.Y. (2016) In Vitro Study of Baicalin Inhibiting the Motor Capacity of Pseudomonas aeruginosa and Synergistic Effect of Combined Cefoperazone/Sulbactam on Biofilm of Pseudomonas aeruginosa. Guangxi Medical University, Nanning. (In Chinese)
Dong, B.Y., Chen, Y.Q., Kong, J.L., et al. (2016) An in Vitro Study on Destructive Effect of Baicalin Combined with Sulbactam-Cefperazone on Pseudomonas aeruginose; Biofilm. Modern Medicine Journal of China, 18, 1-4. (In Chinese)
Bassetti, M., Peghin, M., Vena, A. and Giacobbe, D.R. (2019) Treatment of Infections Due to MDR Gram-Negative Bacteria. Frontiers in Medicine, 6, Article 74. https://doi.org/10.3389/fmed.2019.00074
Le, M.N.-T., Kayama, S., Yoshikawa, M., et al. (2020) Oral Colonisation by Antimicrobial-Resistant Gram-Negative Bacteria among Long-Term Care Facility Residents: Prevalence, Risk Factors, and Molecular Epidemiology. Antimicrobial Resistance & Infection Control, 9, Article No. 45. https://doi.org/10.1186/s13756-020-0705-1
Tehrani, K.H.M.E. and Martin, N.I. (2018) β-Lactam/β-Lactamase Inhibitor Combinations: An Update. MedChemComm, 9, 1439-1456. https://doi.org/10.1039/C8MD00342D
Arakawa, Y., Shibata, N., Shibayama, K., et al. (2000) Convenient Test for Screening Metallo-β-Lactamase-Producing Gram-Negative Bacteria by Using Thiol Compounds. Journal of Clinical Microbiology, 38, 40-43. https://doi.org/10.1128/JCM.38.1.40-43.2000
Gong, M.L., Li, X.X., Zhou, Y., Xu, Y.P. and Cing, Y.L. (2016) Drug Chemosensitivity Test of Etimicin Combined with Cefoperazone/Sulbactam or Piperacillin/Tazobactam against Carbapenem-Resistant Acinetobacter baumannii. Academic Journal of Chinese Pla Medical School, 37, 876-878. (In Chinese)
Li, S.h., Li, D., Yu, F.M., Yang, N. and Liu, Y.F. (2017) In Vitro Antibacterial Activity of Cefoperazone/Sulbactam against Acinetobacter baumannii and Klebsiella pneumonia. Chinese Journal of Nosocomiology, 27, 1452-1455. (In Chinese)
Huang, R. (2018) Evaluation of the Effect of Cefoperazone Sodium and Sulbactam Sodium on Bronchitis. Contemporary Medical Essays, 16, 114-115. (In Chinese)
Xu, A.X., Zeng, X., Hu, Y. and Yu, X. (2019) Antibacterial Effect of Cefoperazone-Sulbactam Sodium Combined with Plantago Extract on Extended Spectrum β-Lactamases Producing Escherichia Coli. Jiangxi Medical Journal, 9, 1034-1036. (In Chinese)
Wang, Q.M., Lu, D.Q., Peng, M., et al. (2019) Clinical Distribution and Drug Resistance Analysis of 2279 Strains of Pseudomonas aeruginosa for 5 Consecutive Years. Journal of Laboratory Medicine and Clinical Medicine, 16, 3437-3440. (In Chinese)
Wang, C.X. (2019) Distribution Characteristics of Pathogenic Bacteria in Children with Acute Upper Respiratory Tract Infection and the Treatment Suggestions. Clinical Medical Research and Practice, 4, 73-74. (In Chinese)
Xu, S.Y. (2019) Comparative Analysis of the Economic Cost-Effectiveness of Different Antibiotics in the Treatment of Acute Upper Respiratory Tract Infections. Journal of Clinical Rational Drug Use, 12, 1-2, 4. (In Chinese)
Li, S.D., Guo, Z.K., Wang, K.Z., Lei, Z. and Zhang, Y. (2021) Comparative Analysis of Two Kinds of Compound Chinese Medicine of Clearing Away Heat and Detoxification in Pharmacological Action, Clinical Application and Adverse Reactions. Journal of Liaoning University of Traditional Chinese Medicine, 23, 126-130. (In Chinese)
Wang, M., Zhang, X.Q., Yang, J., Lin, W.J. and Lin, W.J. (2018) Effect of Sequential Therapy with Azithromycin Combined Shuanghuanglian Oral Liquid on the T Lymphocyte Subsets, Serum Inflammatory Cytokines Levels of Children with Mycoplasma Pneumonia. Hebei Medicine, 24, 1013-1017. (In Chinese)
Wang, B., Wang, R., Guo, L.N., et al. (2021) Anti-Inflammatory Mechanism Analysis of Shuanghuanglian Based on Network Pharmacology. Anhui Medical and Pharmaceutical Journal, 25, 435-440. (In Chinese)
Li, X.F. (2019) Prevention and Treatment of Acute Upper Respiratory Tract Infections in Children. Electronic Journal of Clinical Medical Literature, 6, 44. (In Chinese)
Sun, Y.T. and Wang, N. (2019) Clinical Observation on the Combined Treatment of Traditional Chinese and Western Medicine for Acute Upper Respiratory Tract Infection. Chinese Community Physician, 35, 114, 116. (In Chinese)
Li, N., Liu, Z.Y. and Chen, C.L. (2017) Research Progress on Antibacterial Functions and Mechanisms of Shuanghuanglian. Journal of Yichun University, 39, 16-19. (In Chinese)
Cen, Y.L., Li, Y.N., Kong, J.L., et al. (2017) In Vivo Effect of Baicalin Combined with Meropenem on Early Biofilm of Pseudomonas aeruginosa in Abdominal Cavities of Mice. Chinese Journal of Nosocomiology, 27, 39-42. (In Chinese)
Lu, D.-Y. and Lu, T.-R. (2020) Drug Sensitivity Testing, a Unique Drug Selection Strategy. Advances in Biomarker Sciences and Technology, 2, 59-66. https://doi.org/10.1016/j.abst.2020.11.001
Yin, D., Wu, S., Yang, Y., Shi, Q., Dong, D., Zhu, D. and Hu, F. (2019) Results from the China Antimicrobial Surveillance Network (CHINET) in 2017 of the in Vitro Activities of Ceftazidime-Avibactam and Ceftolozane-Tazobactam against Clinical Isolates of Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother, 63, e02431-18. https://doi.org/10.1128/AAC.02431-18
Qiu, Y.B., Li, L.S., Zhou, F.X., et al. (2020) Detection and Drug Resistance Analysis of 130 Strains of Pseudomonas aeruginosa for Continuous Production of Metallic β-Lactamase, Ultra-Broad Spectrum β-Lactamase and Amp C Enzyme. Journal of Antiinfective Pharmacy, 17, 1464-1466. (In Chinese)
Lu, C.X. (2020) Detection and Drug Resistance Analysis of Ultra Broad Spectrum β-Lactamase Produced by 98 Pseudomonas aeruginosa Isolates from a Hospital. Anti-Infection Pharmacy, 17, 1450-1453. (In Chinese)
Topka-Bielecka, G., Dydecka, A., Necel, A., et al. (2021) Bacteriophage-Derived Depolymerases against Bacterial Biofilm. Antibiotics, 10, Article No. 175. https://doi.org/10.3390/jcm9061832
Zeng, T., Zeng, L., Cao, X., Deng, Q. and Zhang, J. (2019) Antimicrobial Susceptibility and Resistance Genes of Carbapenem-Resistant Pseudomonas aeruginosa. Chinese Journal of Nosocomiology, 29, 3063-3067. (In Chinese)
Zhao, L.J. (2020) Clinical Efficacy and Pharmaceutical Research of Penicillin Combined with Shuanghuanglian in the Treatment of Acute Respiratory Tract Infection. Chinese Journal of Modern Drug Application, 14, 138-139. (In Chinese)
Wang, L.J., Zeng, J.M., Lu, Y., Lan, K., Luo, Q., Lin, D.L., Zhang, W.Z. and Chen, C. (2017) Study on the Effect of Traditional Chinese Medicine on the Efflux Pump of Multidrug-Resistant Pseudomonas aeruginosa. Journal of Molecular Diagnosis and Treatment, 9, 401-407. (In Chinese)
Wang, Z.Z., Liu, S., Li, J.L., et al. (2021) Exploration on Rationality Evaluation Approach of Drug Combination Medication Based on Sequential Analysis and Machine Learning. China Journal of Chinese Materia Medica, 46, 2356-2362. (In Chinese)
Heng, X. (2020) Study on the Efficacy and Changes in Serum Indicators of Shuanghuanglian Oral Liquid Combined with Yanhuning Injection in the Treatment of Viral Pneumonia Patients. Chinese Journal of Modern Drug Application, 14, 180-182. (In Chinese)
Wang, Y., Zhang, D., Wang, H.M., et al. (2016) The Effect of Tanreqing Injection on the Biofilm Formation of Pseudomonas aeruginosa. Chinese Journal of Nosocomiology, 26, 4841-4843. (In Chinese)
Ionescu, C.M., Ghita, M., Copot, D., Derom, E. and Verellen, D. (2020) A Minimal PKPD Interaction Model for Evaluating Synergy Effects of Combined NSCLC Therapies. Journal of Clinical Medicine, 9, Article No. 1832. https://doi.org/10.3390/jcm9061832