Bioremediation of Textile Azo Dyes Amido Black 10B, Reactive Black 5, Reactive Blue 160 by <i>Lentinus squarrosulus</i> AF5 and Assessment of Toxicity of the Degraded Metabolites — Oak Academic Publishing
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Bioremediation of Textile Azo Dyes Amido Black 10B, Reactive Black 5, Reactive Blue 160 by <i>Lentinus squarrosulus</i> AF5 and Assessment of Toxicity of the Degraded Metabolites
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
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Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
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Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
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Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
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Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
1 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
2 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
3 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
4 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
5 Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, India
Bioremediation is an eco-compatible and economical approach to counter textile dye menace. The isolated Lentinus squarrosulus AF5 was assessed for decolourization of textile azo dyes, and had shown ~93%, 88% and 70% decolorization of Reactive blue 160 (RB160), Reactive black 5 (RB5) and Amido black 10B (AB10B) respectively. Further analysis using UV-vis, HPLC, and FTIR, 1 H NMR had shown the degradation of the dyes. Toxicity analysis of the metabolites was performed using seed germination and plant growth on two agriculturally important plants Guar ( Cyamopsis tetragonoloba ) and wheat ( Triticum aestivum ) as well as cytotoxicity analysis using the human keratinocyte cell line (HaCaT). The dye mix appeared inhibitory for seed germination (20% - 40%), whereas metabolites were non-inhibitory for germination. Treatment of HaCaT cells with of dye mix and metabolites led into 45% and ~100% of cell viability of HaCaT cells respectively. Therefore, metabolites following degradation of the dye mix were observed to be non-toxic.
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