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Cytotoxic and Cell Proliferation Activities of Isocoumarin Derivatives Isolated from Phialocephala scopiformis FC-1873
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
Department of Botany, National Museum of Nature and Science, Ibaraki, Japan
Department of Botany, National Museum of Nature and Science, Ibaraki, Japan
School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
Faculty of Pharmacy and Pharmaceutical Sciences, School of Pharmacy, Josai University, Saitama, Japan
Faculty of Pharmacy, Juntendo University, Chiba, Japan
River Museum of Saitama, Saitama, Japan
- 1 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 2 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 3 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 4 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 5 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 6 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 7 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 8 Department of Botany, National Museum of Nature and Science, Ibaraki, Japan
- 9 Department of Botany, National Museum of Nature and Science, Ibaraki, Japan
- 10 School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Tokyo, Japan
- 11 Faculty of Pharmacy and Pharmaceutical Sciences, School of Pharmacy, Josai University, Saitama, Japan
- 12 Faculty of Pharmacy, Juntendo University, Chiba, Japan
- 13 River Museum of Saitama, Saitama, Japan
Advances in Microbiology·Volume 15 (2025)·Pages 284–295·Published 14 May 2025·DOI10.4236/aim.2025.155020
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Abstract
Phialocephala scopiformis (Ascomycota, Helotiales, Mollisiaceae) are fungi known as both saprophytes and endophytes, forming small apothecia (1 - 3 mm in diameter) on decaying wood. In this study, three novel isocoumarin derivatives (+)-phaeosphaerin A ( 1 ), (+)-phaeosphaerin B ( 2 ), and ( S )-6-demethylkigelin ( 3 ) were isolated from Phialocephala scopiformis FC-1873 collected in Japan, along with two known isocoumarins, lignicol ( 4 ) and 6,8-dihydroxy-3,5-dimethyl-1 H -2-benzopyran-1-one ( 5 ). The cytotoxicity of 1 and 4 were evaluated against HL-60, PANK-1, HepG2, HT-29, and T98G cell lines, as well as their effects on cell proliferation in the skin keratinocyte cell line HaCaT. These findings highlight the potential of mollisioid fungi as a source of novel bioactive compounds.
Keywords(+)-Phaeosphaerin A( S )-6-DemethylkigelinCytotoxicityCell Proliferation EffectPhialocephala scopiformis
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