Background: “Kossam”, a traditionally processed milk in Cameroon and sub-Saharan Africa, has been described as potential source of probiotic lactic acid bacteria (LAB). However, to our knowledge, no research work has been done on their immunomodulatory properties. Aim: This study was aimed at isolating and characterising lactic acid bacteria from “Kossam”, a traditionally fermented cow milk and investigating their immunomodulatory properties in rat model. Materials and Methods: Lactic acid bacteria were isolated from “Kossam” using pour plating technique. The strains exhibiting probiotic potential were selected and their identities were confirmed using the sequencing of 16S ribosomal RNA gene. Probiotic isolates were administered to rats for 2 consecutive days and a post ingestion period of 10 days was observed. Full blood count, differential blood count, total spleen protein and pro-inflammatory cytokines (TNF- α , IFN-g, IL-1- β and IL-6) concentrations were detected in the sera after 10 days. Results: A significant increase (p < 0.05) in the level of RBCs, lymphocytes, monocytes and total spleen proteins was observed in rats treated with the different isolates. However, a significant decrease (p < 0.05) in circulating WBCs and neutrophils count was observed in animals treated with the isolates A20, A27, A46, and A56 that had the highest effect (p < 0.05) on pro-inflammatory cytokines level in serum. These strains were identified using 16S rRNA gene sequence as strains of Lacticaseibacillus paracasei (A20), Limosilactobacillus fermentum (A27), Enterococcus faecium (A46), Lactiplantibacillus plantarum (A56). Conclusion: These isolates showed immunomodulatory properties in rats and were able to prevent the incidence of diarrhoea associated with purgative components.
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