Hygiene and Toxi-Infectious Risks Associated with Tchakpalo , a Traditional Beninese Beer — Oak Academic Publishing
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Hygiene and Toxi-Infectious Risks Associated with Tchakpalo , a Traditional Beninese Beer
Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
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Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
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Laboratory of Applied Genetics, Biotechnology and Botany (GEBBA), National Higher School of Biosciences and Applied Biotechnologies, National University of Sciences, Technologies, Engineering and Mathematics, Benin
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Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
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Laboratory of Applied Genetics, Biotechnology and Botany (GEBBA), National Higher School of Biosciences and Applied Biotechnologies, National University of Sciences, Technologies, Engineering and Mathematics, Benin
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Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
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National Center for Agronomic Research, Research Station of Ferkessédougou, Ferkessédougou, Ivory Coast
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Communicable Disease Research Unit, Applied Research Laboratory, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin
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Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
1 Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
2 Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
3 Laboratory of Applied Genetics, Biotechnology and Botany (GEBBA), National Higher School of Biosciences and Applied Biotechnologies, National University of Sciences, Technologies, Engineering and Mathematics, Benin
4 Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
5 Laboratory of Applied Genetics, Biotechnology and Botany (GEBBA), National Higher School of Biosciences and Applied Biotechnologies, National University of Sciences, Technologies, Engineering and Mathematics, Benin
6 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
7 National Center for Agronomic Research, Research Station of Ferkessédougou, Ferkessédougou, Ivory Coast
8 Communicable Disease Research Unit, Applied Research Laboratory, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin
9 Laboratory of Microbiology, Food Technology and Phytopathology, Faculty of Science and Technology, University of Abomey-Calavi, Abomey-Calavi, Benin
Tchakpalo is a traditional Beninese beer produced through the spontaneous fermentation of cereals, primarily maize and sorghum. Despite its socio-economic, cultural, and nutritional importance, the conditions under which it is produced and marketed raise concerns regarding its food safety. The present study aimed to assess the microbiological quality of Tchakpalo sold in Grand Nokoué (Benin) and to evaluate the level of Good Hygiene Practices (GHP) adoption by producers. Fifty samples of Tchakpalo were therefore collected in five municipalities of Grand Nokoué (Cotonou, Porto-Novo, Abomey-Calavi, Sèmè-Podji, and Ouidah). Microbiological analyses focused on Total Mesophilic Aerobic Flora (TMAF), Enterobacteria, E. coli , S. aureus , and molds. Additionally, the adoption level of GHP was assessed through direct observation of sixty-one Tchakpalo producers. The results revealed widespread microbiological contamination of Tchakpalo . The prevalence of TMAF and Enterobacteria was 100%, while those of molds, E. coli , and S. aureus were 80%, 62%, and 52%, respectively. The average microbial loads were 6.57 × 10 6 CFU/mL for TMAF, 5.21 × 10 3 CFU/mL for Enterobacteria, 3.44 × 10 3 CFU/mL for E. coli , 0.75 × 10 3 CFU/mL for S. aureus , and 2.51 × 10 2 CFU/mL for molds. Interpretation of these results considering regulatory guidelines showed that a significant proportion of the samples exhibited poor to unsatisfactory microbiological quality. The assessment of GHPs revealed serious deficiencies at all levels of the production and marketing chain, notably the lack of adequate handwashing, failure to wear protective equipment, the use of untreated water, improper storage of raw materials and the finished product, as well as the lack of training and health monitoring for producers. These results demonstrate that the Tchakpalo sold in the Grand Nokoué area presents a concerning level of sanitary quality, strongly associated with the low level of adoption of GHP. Training initiatives, health supervision, and process standardization appear essential to reduce the risks of foodborne illnesses linked to the consumption of this emblematic traditional Beninese beverage.
Motlhanka, K., Lebani, K., Boekhout, T. and Zhou, N. (2020) Fermentative Microbes of Khadi, a Traditional Alcoholic Beverage of Botswana. Fermentation , 6, Article 51. https://doi.org/10.3390/fermentation6020051
Bayoï, J.R. and Etoa, F. (2021) Assessment of Microbiological Quality and Safety during the Processing of Traditional Beers Made from Sorghum in the “Mandara” Mountains of the Far-North Region of Cameroon. European Journal of Biology and Biotechnology , 2, 74-84. https://doi.org/10.24018/ejbio.2021.2.2.156
Christelle, K.A.B.M., Clément, K.K., Athanase, K.K., Ibrahim, K. and Zinzendorf, N.Y. (2026) Study of the Microbiological Quality of Artisanal Soft Drinks (Bissap, Gnamankoudji) Produced in the Haut Sassandra Region, Daloa Department, Ivory Coast. Journal of Advances in Microbiology , 26, 130-143. https://doi.org/10.9734/jamb/2026/v26i21079
Aboagye, G., Gbolonyo-Cass, S., Kortei, N.K. and Annan, T. (2020) Microbial Evaluation and Some Proposed Good Manufacturing Practices of Locally Prepared Malted Corn Drink (“Asaana”) and Hibiscus Sabdarifa Calyxes Extract (“Sobolo”) Beverages Sold at a University Cafeteria in Ghana. Scientific African , 8, e00330. https://doi.org/10.1016/j.sciaf.2020.e00330
Dognon, D., Damien Pacôme Noumavo, A., Vodouhe, S., Adjovi, L., Bravo, J., Yaï Akin, Y., et al . (2026) Diversity of Traditional Processing of Tchakpalo , an Indigenous Beer from Benin. American Journal of Food Science and Technology , 14, 63-73. https://doi.org/10.12691/ajfst-14-3-1
SIRAT (2025) Procédures de gestion de la main-d’œuvre (PGMO): Projet de Mo-bilité Urbaine Durable du Grand Nokoué (PMUD-GN). Report, Société des Infra-structures Routières et de l’Aménagement du Territoire (SIRAT), Cotonou, Bénin. https://www.aiib.org/en/projects/details/2025/_download/Benin/Labor-Management-Procedures-LMP-French.pdf
Tétédé Rodrigue Konfo, C., Worou Chabi, N., Dahouenon-Ahoussi, E., Cakpo-Chichi, M., Mansourou Soumanou, M. and Coco Kodjo Sohounhloue, D. (2015) Improvement of African Traditional Sorghum Beers Quality and Potential Applications of Plants Extracts for Their Stabilization: A Review. Journal of Microbiology , Biotechnology and Food Sciences , 5, 190-196. https://doi.org/10.15414/jmbfs.2015.5.2.190-196
Speck, M.L. (1976) Compendium of Methods for the Microbiological Examination of Foods. American Public Health Association.
ISO4833-2 (2013) ISO 4833-2: Microbiology of the Food Chain—Horizontal Method for the Enumeration of Microorganisms—Part 2: Colony Count at 30˚C by the Surface Plating Technique.
ISO21528-1 (2017) ISO 21528-1: Microbiology of the Food Chain—Horizontal Method for the Detection and Enumeration of Enterobacteriaceae—Part 1: Detection of Enterobacteriaceae.
ISO16649-2 (2001) ISO 16649-2: Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Enumeration of β -Glucuronidase-Positive Escherichia coli —Part 2: Colony-Count Technique at 44˚C Using 5-Bromo-4-Chloro-3-Indolyl β -D-Glucuronide.
ISO6888-1 (2021) ISO 6888-1: Microbiology of the Food Chain—Horizontal Method for the Enumeration of Coagulase-Positive Staphylococci ( Staphylococcus aureus and Other Species)—Part 1: Method Using Baird-Parker Agar Medium.
ISO21527-2 (2008) ISO 21527-2: Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Enumeration of Yeasts and Moulds—Part 2: Colony Count Technique in Products with Water Activity Less than or Equal to 0.95.
MAPAQ (2019) Lignes directrices et normes pour l’interprétation des résultats an-alytiques en microbiologie alimentaire. Report, Ministère de l’Agriculture, des Pêcheries et de l’Alimentation du Québec (MAPAQ), Québec. http://www.mapaq.gouv.qc.ca/fr/Publications/recueil.pdf
FSANZ (2025) Compendium of Microbiological Criteria for Food (July 2025 Edition). Report, Food Standards Australia New, Zealand (FSANZ), Canberra; Wellington. https://www.foodstandards.gov.au
Chaves-López, C. and Cordero-Bueso, G. (2022) Microbial Diversity and Safety in Fermented Beverages. Beverages , 8, Article 14. https://doi.org/10.3390/beverages8010014
Adekoya, I., Obadina, A., Olorunfemi, M., Akande, O., Landschoot, S., De Saeger, S., et al . (2019) Occurrence of Bacteria and Endotoxins in Fermented Foods and Beverages from Nigeria and South Africa. International Journal of Food Microbiology , 305, Article ID: 108251. https://doi.org/10.1016/j.ijfoodmicro.2019.108251
Azanaw, J., Engdaw, G.T., Dejene, H., Bogale, S. and Degu, S. (2022) Food Hygiene Knowledge, and Practices and Their Associated Factors of Street Food Vendors in Gondar City, Northwest Ethiopia, 2021: A Cross-Sectional Study. Heliyon , 8, e11707. https://doi.org/10.1016/j.heliyon.2022.e11707
Kadyan, S., Kumar, N., Lawaniya, R., Sharma, P.K., Arora, B. and Tehri, N. (2020) Rapid and Miniaturized Method for Detection of Hygiene Indicators, Escherichia coli and Coliforms, in Dairy Products. Journal of Food Safety , 40, e12839. https://doi.org/10.1111/jfs.12839
Wang, Y., Zhang, J., Peng, Z., Wang, X. and Plummer, P. (2023) Editorial: Food Pathogens and Antimicrobial Resistance. Frontiers in Microbiology , 14, Article 1243769. https://doi.org/10.3389/fmicb.2023.1243769
Baidoo, M.F., Oduro, I., Ellis, W.O. and Saalia, F.K. (2023) Microbiological Quality and Safety Assessment of Emuduro, a Traditional Fermented Cereal-Based Beverage Consumed in Ghana. Food Research , 7, 120-128.
Lynch, K.M., Wilkinson, S., Daenen, L. and Arendt, E.K. (2021) An Update on Water Kefir: Microbiology, Composition and Production. International Journal of Food Microbiology , 345, Article ID: 109128. https://doi.org/10.1016/j.ijfoodmicro.2021.109128
Noumavo, A.D.P., Sina, H., Tovide, N., Dognon, D.A.Y., Houessou, S.K., Konfo, C.T.R., Hossou, E., Toukourou, F. and Baba-Moussa, F. (2022) Manufacturing Process and Hygienic and Technical Shortcomings of Fruit Juices Production in Southern Benin. American Journal of Food Science and Technology , 10, 200-206.
Fernandes, A., Ramos, C., Monteiro, V., Santos, J. and Fernandes, P. (2022) Virulence Potential and Antibiotic Susceptibility of S. Aureus Strains Isolated from Food Handlers. Microorganisms , 10, Article 2155. https://doi.org/10.3390/microorganisms10112155
Páez-Lerma, J.B., Gschaedler-Mathis, A.C. and Lopes, C.A. (2024) Editorial: Microbial Communities of Traditional Fermented Beverages: Physiology, Metabolism and Interactions in Fermentative Processes. Frontiers in Microbiology , 15, Article 1480982. https://doi.org/10.3389/fmicb.2024.1480982
Peterson, E. and Kaur, P. (2018) Antibiotic Resistance Mechanisms in Bacteria: Relationships between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens. Frontiers in Microbiology , 9, Article 2928. https://doi.org/10.3389/fmicb.2018.02928
Petro, C.D., Duncan, J.K., Seldina, Y.I., Allué-Guardia, A., Eppinger, M., Riddle, M.S., et al . (2020) Genetic and Virulence Profiles of Enteroaggregative Escherichia coli (EAEC) Isolated from Deployed Military Personnel (DMP) with Travelers’ Diarrhea. Frontiers in Cellular and Infection Microbiology , 10, Article 200. https://doi.org/10.3389/fcimb.2020.00200
Abu-Sini, M.K., Maharmah, R.A., Abulebdah, D.H. and Al-Sabi, M.N.S. (2023) Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan. Healthcare , 11, Article 299. https://doi.org/10.3390/healthcare11030299
Baraily, P., Zende, R.J., Kshirsagar, D.P., Vaidya, V.M., Waghamare, R.N., Paturkar, A.M., et al . (2018) Rapid Detection of Shiga Toxin-Producing E . coli in Animal Origin Foods Using Loop-Mediated Isothermal Amplification (LAMP) Assay. Agricultural Research , 8, 490-496. https://doi.org/10.1007/s40003-018-0391-x
Zhou, Y., Zhu, X., Hou, H., Lu, Y., Yu, J., Mao, L., et al . (2018) Characteristics of Diarrheagenic Escherichia coli among Children under 5 Years of Age with Acute Diarrhea: A Hospital Based Study. BMC Infectious Diseases , 18, Article No. 63. https://doi.org/10.1186/s12879-017-2936-1
Ananya, P., Sayan, B. and Shibani, L. (2025) Staphylococcus aureus Food Poisoning: An Overview. RFP Indian Journal of Hospital Infection , 7, 43-47. https://rfppl.co.in/public/uploads/article_issue/1772445011_69a55d53c75e0.pdf?srsltid=AfmBOoqt82A05zc9DFmx2uZCAnMkJQak6tMM5Uij6GSOCGgDJf8l67B0
Erkihun, Y., Molla, G., Getachew, B., Eshetu, Y., Gedefie, A., Achame, M.S., et al . (2025) Prevalence of Staphylococcus aureus among Food Handlers in Ethiopia, 2000-2024: A Systematic Review and Meta-Analysis. BMC Infectious Diseases , 26, Article No. 29. https://doi.org/10.1186/s12879-025-12260-z
Attien, P.Y., Koua, A., Chabi-Sika, K., Sina, H., Konate, I. and Baba-Moussa, L. (2021) Prevalence of Staphylococcus aureus Isolated from Some Street Hot Beverages in Abidjan, Côte d’Ivoire. Asian Food Science Journal , 20, 48-55. https://doi.org/10.9734/afsj/2021/v20i1130375
Chebana, K.E.B. and Talbi, C. (2021) Staphylococcus aureus dans les viandes rouges et résistance aux antibiotiques. Master’s Thesis, Université Frères Mentouri Constantine 1.
Fetsch, A. and Johler, S. (2014) Staphylococcus aureus as a Foodborne Pathogen. Toxins , 6, 2714-2735.
Murray, P.R., Rosenthal, K.S. and Pfaller, M.A. (2005) Medical Microbiology. 5th Edition, Elsevier.
Konan, K.H., Bouatenin, K.M.J.P., Kouamé, K.A. and Djè, K.M. (2016) Évaluation de la qualité microbiologique et physico-chimique de boissons fermentées tradition-nelles locales. Journal of Applied Biosciences , 102, 9720-9731.
Pitt, J.I. and Hocking, A.D. (2009) Fungi and Food Spoilage. Springer.
Pandey, A.K., Samota, M.K., Kumar, A., Silva, A.S. and Dubey, N.K. (2023) Fungal Mycotoxins in Food Commodities: Present Status and Future Concerns. Frontiers in Sustainable Food Systems , 7, Article 1162595. https://doi.org/10.3389/fsufs.2023.1162595
Haque, M.A., Wang, Y., Shen, Z., Li, X., Saleemi, M.K. and He, C. (2020) Mycotoxins in Food and Feed: Toxicity, Preventive Challenges, and Control Strategy. Toxins , 12. Article 698.
Chelule, P.K., Mokoena, M.P. and Gqaleni, N. (2010) Advantages of Traditional Lactic Acid Bacteria Fermentation of Food in Africa. African Journal of Biotechnology , 9, 249-258.
Holzapfel, W.H. (2002) Appropriate Starter Culture Technologies for Small-Scale Fermentation in Developing Countries. International Journal of Food Microbiology , 75, 197-212. https://doi.org/10.1016/s0168-1605(01)00707-3
Tamang, J.P., Watanabe, K. and Holzapfel, W.H. (2016) Review: Diversity of Microorganisms in Global Fermented Foods and Beverages. Frontiers in Microbiology , 7, Article 377. https://doi.org/10.3389/fmicb.2016.00377
Tankoano, A., Diop, M., Lingani, H., Kaoré, D. and Savadogo, A. (2017) Les aspects technologiques, microbiologiques et nutritionnels des aliments fermentes a base de lait et de mil en afrique de l’ouest. International Journal of Advanced Research , 5, 1509-1526. https://doi.org/10.21474/ijar01/5211
Byakika, S., Mukisa, I.M., Byaruhanga, Y.B., Male, D. and Muyanja, C. (2019) Influence of Food Safety Knowledge, Attitudes and Practices of Processors on Microbiological Quality of Commercially Produced Traditional Fermented Cereal Beverages, a Case of Obushera in Kampala. Food Control , 100, 212-219. https://doi.org/10.1016/j.foodcont.2019.01.024
Olsen, M., Campos, M., Lohning, A., Jones, P., Legget, J., Bannach-Brown, A., et al . (2020) Mobile Phones Represent a Pathway for Microbial Transmission: A Scoping Review. Travel Medicine and Infectious Disease , 35, Article ID: 101704. https://doi.org/10.1016/j.tmaid.2020.101704
Islam, M.S., Ahmed, F., Rahman, M.M., Hasan, M.K. and Khan, M.A. (2025) Bacterial Contamination and Multidrug-Resistant Profiles of Circulating Coins in Bangladesh. Journal of Preventive Medicine and Hygiene , 66, E12-E20.
Appiah, P.O., Odoom, A., Tetteh-Quarcoo, P.B. and Donkor, E.S. (2026) Money and Microbes: A Global Systematic Review and Meta-Analysis of Currency Contamination. Environmental Health Insights , 20, 1-15. https://pmc.ncbi.nlm.nih.gov/articles/PMC12813264/
Tang, J., Peng, L., Ali, A., Zhao, S., Zeng, Z., Yuan, K., et al . (2024) Electrochemical Detection of Rutin in Black Tartary Buckwheat Tea and Related Health-Care Pills with New Ionic Liquid-Based Supramolecular Hydrogels. Food Control , 155, Article ID: 110045. https://doi.org/10.1016/j.foodcont.2023.110045
Khoothiam, K., Prapasawat, W., Yosboonruang, A., Rawangkan, A., Phuangsri, C., Rupprom, K., et al . (2023) Prevalence, Antimicrobial Resistance, and Enterotoxin Gene Profiles of Staphylococcus aureus Isolated from Mobile Phones of the Food Vendors in Phayao Province, Thailand. Annals of Clinical Microbiology and Antimicrobials , 22, Article No. 68. https://doi.org/10.1186/s12941-023-00621-y
Seki, V., Kouamé, K.A., Koffi, E.K. and Djè, K.M. (2024) Evaluation of the Microbiological Quality of Drinking Water from Source to Household Storage in Côte d’Ivoire. Environmental Health Insights , vol. 18.
Lapworth, D.J., Nkhuwa, D.C.W., Okotto-Okotto, J., Pedley, S., Stuart, M.E., Tijani, M.N., et al . (2017) Urban Groundwater Quality in Sub-Saharan Africa: Current Status and Implications for Water Security and Public Health. Hydrogeology Journal , 25, 1093-1116. https://doi.org/10.1007/s10040-016-1516-6
Olalemi, A.S., Adeyemo, O.K. and Bakare, A.A. (2023) Microbiological Assessment of Groundwater Quality and Prevalence of Fecal Indicators in Nigeria. Water Science and Technology , 88, 1021-1035.
Justin Behanzin, G., Sako Avocefohoun, A., Theotime Francis Hounsou, M., Vodouhè, S., Sourou Montcho, P., Damien Zannou, S., et al . (2024) Physico-Chemical Characterization of Well and Borehole Water in the Municipality of Seme-Podji. American Journal of Water Resources , 12, 98-105. https://doi.org/10.12691/ajwr-12-3-5
Adanlokonon, E.S., Dougnon, V.T., Deguenon, E. and Baba-Moussa, F. (2025) Évaluation de la pollution fécale des eaux de puits de la commune d’Agbangnizoun, Bénin. Journal de la Société Ouest-Africaine de Chimie , 57, 45-53.
Mishra, R., Kumar, A. and Gupta, S. (2024) Principles of Food Safety and Storage Management in Small-Scale Food Enterprises. Food Quality and Safety , 8, fqae012.
Sikombe, T.W., Moonga, H.B., Linnemann, A.R., Smid, E.J. and Schoustra, S.E. (2025) The Effect of Storage Temperature on the Physicochemical Characteristics, Volatile Compounds and Microbial Community of a Traditionally Fermented Dairy Product. LWT , 227, Article ID: 118035. https://doi.org/10.1016/j.lwt.2025.118035
Hilarion Ahehehinnou, U., Christie Sissinto Adjovi, Y. and Prince Mintognisse Fossou, J. (2024) Microbiological Quality of Ready-to-Eat Foodsin Benin: Biological Contaminantand Health Risks. International Journal of Advanced Research , 12, 518-533. https://doi.org/10.21474/ijar01/19862
Fekadu, G., Tazebew, A. and Alemayehu, M. (2024) Compliance with Food Safety Requirements among Food Establishments: The Role of Regular Sanitary Inspections. BMC Public Health , 24, Article No. 842.
Oliveira, I., Almeida, M., Gomes, J.J.F. and Henriques, A.R. (2024) Specific Personal Hygiene Procedures and Practices in Food Handlers—A Cross-Sectional Study in Butcher and Fishmonger Shops in Almada. Hygiene , 4, 207-220. https://doi.org/10.3390/hygiene4020017