Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK — Oak Academic Publishing
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Anti-Inflammatory Effect of 3-Methylcarbazoles on RAW 264.7 Cells Stimulated with LPS, Polyinosinic-Polycytidylic Acid and Pam3CSK
Department of Microbiology, Faculty of Science, Silpakorn University, Bangkok, Thailand
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Department of Microbiology, Faculty of Science, Silpakorn University, Bangkok, Thailand
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Department of Chemistry, Faculty of Science, Silpakorn University, Bangkok, Thailand
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Department of Chemistry, Faculty of Science, Silpakorn University, Bangkok, Thailand
1 Department of Microbiology, Faculty of Science, Silpakorn University, Bangkok, Thailand
2 Department of Microbiology, Faculty of Science, Silpakorn University, Bangkok, Thailand
3 Department of Chemistry, Faculty of Science, Silpakorn University, Bangkok, Thailand
4 Department of Chemistry, Faculty of Science, Silpakorn University, Bangkok, Thailand
In the present study, 3-methylcarbazole and 1-methoxy-3-methylcarbazole were isolated from the culture of Streptomyces sp. LJK109, endophyte of Alpinia galanga Swartz. 3-methylcarbazole, a carbazole derivative, has been found to be highly potent as anti-inflammatory agent. The immunomodulatory activity of these agents in toll like receptor (TLR)-activated RAW 264.7 macrophages induced by lipopolysaccharide (LPS), Poly(I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and pro-inflammatory cytokines. The 3-methylcarbazoles dose-dependently suppressed the release of NO, PGE2, TNF- α , IL-1 β , IL-6 and IL-10 in LPS- and pam3CSK-activated macrophages but not in Poly(I:C)-activated macrophages. Our results suggest that 3-methylcarbazoles can be further developed as a promising anti-inflammatory remedy.
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