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Activation of Ethanol Production by Combination of Recombinant <i>Ralstonia eutropha</i> and Electrochemical Reducing Power
Department of Biological Engineering, Seokyeong University, Seoul, South Korea
Department of Biological Engineering, Seokyeong University, Seoul, South Korea
Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon, South Korea
Department of Biological Engineering, Seokyeong University, Seoul, South Korea
- 1 Department of Biological Engineering, Seokyeong University, Seoul, South Korea
- 2 Department of Biological Engineering, Seokyeong University, Seoul, South Korea
- 3 Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon, South Korea
- 4 Department of Biological Engineering, Seokyeong University, Seoul, South Korea
Advances in Microbiology·Volume 03 (2013)·Pages 42–45·Published 27 March 2013·DOI10.4236/aim.2013.31006
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Abstract
Ralstonia eutropha was genetically modified to induce ethanol production from glucose. An electrochemical bioreactor was prepared to generate electrochemical reducing power coupled to regeneration of NADH. Growing cells of recom binant R. eutropha produced about 29 mM of ethanol in conventional conditions and 56 mM of ethanol in electro chemically reduced conditions from 100 mM glucose. Grown cells of the recombinant produced about 52 mM of etha nol in conventional conditions and 142 mM of ethanol in electrochemically reduced condition from 100 mM glucose. These results are a clue that electrochemical reducing power can induce the recombinant R. eutropha to produce more ethanol coupled to increase of NADH/NAD + ratio.
Keywords<i>Ralstonia eutropha</i>Electrochemical Reducing PowerGenetic RecombinationPyruvate DecarboxylaseAlcohol Dehydrogenase
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