Detection of 232bp Virulent Gene of Pathogenic <i>Aeromonas hydrophila</i> through PCR Based Technique: (<i>A Rapid Molecular Diagnostic Approach</i>) — Oak Academic Publishing
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Detection of 232bp Virulent Gene of Pathogenic <i>Aeromonas hydrophila</i> through PCR Based Technique: (<i>A Rapid Molecular Diagnostic Approach</i>)
Department of Biotechnology, The University of Burdwan, Burdwan, India
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Department of Biotechnology, Oriental Institute of Science and Technology, Burdwan, India
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Department of Biotechnology, The University of Burdwan, Burdwan, India
1 Department of Biotechnology, The University of Burdwan, Burdwan, India
2 Department of Biotechnology, Oriental Institute of Science and Technology, Burdwan, India
3 Department of Biotechnology, The University of Burdwan, Burdwan, India
The pathogenicity of aeromonads produces due to exotoxins such as cytolytic enterotoxin, hemolysin or aerolysin, li pases and proteases. Rapid detection of A. hydrophila cytolytic enterotoxin (AHCYTONE) gene and their characteriza tion has proven importance so that proper and rapid preventive and control measures could be taken up to reduce mor tality and loss in fish culture. The main objective of the present study is to genetic identification of AHCYTONE posi tive Aeromonas hydrophila. Strains were isolated from fishes from different fish market in West Bengal and water sam ples from different river and ponds. Initially strains were identified by their phenotypic and biochemical characteriza tion. Due to contradiction of those results, molecular characterization was done by polymerase chain reaction, which is proved a suitable and rapid diagnostic tool for identification and characterization of A. hydrophila . We have also evalu ated the potential risk to human health that this finding can represent by determining the presence of the cytolytic en terotoxin gene in such isolates.
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