Isolation and Identification of Two Antibacterial Agents from <i>Chromolaena odorata</i> L. Active against Four Diarrheal Strains — Oak Academic Publishing
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Isolation and Identification of Two Antibacterial Agents from <i>Chromolaena odorata</i> L. Active against Four Diarrheal Strains
Inserm UMR 1121, University of Strasbourg, Strasbourg, France
,
Université d’Abomey-Calavi, Laboratoire de Biochimie et Biologie Moléculaire (LBBM), Cotonou, Bénin
,
Inserm UMR 1121, University of Strasbourg, Strasbourg, France
,
CNRS UMR7178, University of Strasbourg, Strasbourg, France
,
IPHC-DSA, CNRS, University of Strasbourg, Strasbourg, France
,
CNRS UMR7178, University of Strasbourg, Strasbourg, France
,
IPHC-DSA, CNRS, University of Strasbourg, Strasbourg, France
,
EA 7290, University of Strasbourg, Strasbourg, France
,
Inserm UMR 1121, University of Strasbourg, Strasbourg, France
,
Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France
,
Université d’Abomey-Calavi, Laboratoire de Biochimie et Biologie Moléculaire (LBBM), Cotonou, Bénin
,
Inserm UMR 1121, University of Strasbourg, Strasbourg, France
1 Inserm UMR 1121, University of Strasbourg, Strasbourg, France
2 Université d’Abomey-Calavi, Laboratoire de Biochimie et Biologie Moléculaire (LBBM), Cotonou, Bénin
3 Inserm UMR 1121, University of Strasbourg, Strasbourg, France
4 CNRS UMR7178, University of Strasbourg, Strasbourg, France
5 IPHC-DSA, CNRS, University of Strasbourg, Strasbourg, France
6 CNRS UMR7178, University of Strasbourg, Strasbourg, France
7 IPHC-DSA, CNRS, University of Strasbourg, Strasbourg, France
8 EA 7290, University of Strasbourg, Strasbourg, France
9 Inserm UMR 1121, University of Strasbourg, Strasbourg, France
10 Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg, France
11 Université d’Abomey-Calavi, Laboratoire de Biochimie et Biologie Moléculaire (LBBM), Cotonou, Bénin
12 Inserm UMR 1121, University of Strasbourg, Strasbourg, France
Chromolaena odorata L (Asteraceae) is a bad invasive plant, found in the humid tropics and sub-tropics worldwide. It is used against dysentery, diarrhea, malaria, wound healing, headache and toothache in traditional medicine. In the pre sent study, we investigated the antibacterial activities of different leaves extracts of Chromolaena odorata L. (cyclo hexane, dichloromethane, ethyl acetate and butanol) against four clinical diarrheal strains ( Klebsiella oxytoca , Salmo nella enterica , Shigella sonnei and Vibrio cholera ). We demonstrated that C. odorata leaves extracts show an antibacte rial activity between 0.156 and 1.25 mg/mL. Bioassay-guided chromatography by bioautography with iodonitrotetra zolium-based colorimetric assay allowed the isolation and identification of two active compounds. After the combina tion of RP-HPLC, mass spectrometry analysis , 1D and 2D-NMR spectroscopy, we isolated and characterized two active molecules corresponding to 3’,4’,5,6,7-Pentamethoxyflavone (Sinensetin) and4 ’ ,5,6,7-Tetramethoxyflavone ( Scutel lareintetramethyl ether) .
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Antibacterial Activity
Diarrheal Strains
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