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Hyphal Morphology and Elongation Alterations in <i>Aspergillus nidulans</i> Provoked by the Diterpene Kaurenoic Acid
Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto, Brazil
- 1 Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto, Brazil
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Advances in Microbiology·Volume 03 (2013)·Pages 438–441·Published 16 August 2013·DOI10.4236/aim.2013.35059
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Abstract
Kaurenoic acid (KA) , a kaurane-type diterpene extracted from leaves of Mikania hirsutissima , was previously reported as an inhibitor of vascular contractility mainly by blocking extracellular Ca 2+ influx. The compound is known for several other biological activities such as antiparasitic, antispasmodic and antibacterial activity. The aim of the present study was to investigate the effect of KA on Aspergillus nidulans . KA ( 0.3 mM ) showed fungistatic activity against A. nidulans with visible hyphal elongation and morphology damage. These effects were reverted by CaCl 2 addition showing that KA interferes with intracellular Ca 2+ gradient in A. nidulans. This is the first report on the mechanism of action of KA involving calcium levels by altering the elongation of fungi hyphae.
Keywords<i>Aspergillus nidulans</i>Calcium GradientFungistatic ActivityKaurenoic Acid
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