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Virulence Factors in Methicillin-Resistant <i>Staphylococcus aureus</i> Isolated from ICU Units in Brazil

¹ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
² Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de S?o Paulo, S?o Paulo, Brazil
³ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
⁴ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
⁵ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
⁶ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
⁷ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
⁸ Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de S?o Paulo, S?o Paulo, Brazil
⁹ Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de S?o Paulo, S?o Paulo, Brazil
¹⁰ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
¹¹ Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil
¹² Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil;Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de S?o Paulo, S?o Paulo, Brazil

Advances in Microbiology·Volume 04 (2014)·Pages 207–215·Published 12 March 2014·DOI10.4236/aim.2014.44027

Abstract

Species of Staphylococcus are common in hospital infection (HI). Methicillin resistant S. aureus (MRSA) has also become a serious problem in Brazilian HI. The aim of this study was to characterize the pathogenicity of methicillin-resistant S. aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) isolated in public hospitals. The clinical isolates were obtained from intensive care unit. The MRSA and MSSA strains were genotyped by PCR for detection genes related to virulence factors. Moreover, the strains were tested for biofilm formation and cytokine induction in macrophages. Three strains of MRSA (9.68%) expressed the Sea gene, one (3.23%) Seb, 17 (54.84%) Spa and seven (22.58%) had PVL. Two MSSA strains (2.98%) expressed the Sea gene, three (4.48%) Seb, 18 (26.87%) Spa and 11 (16.42%) showed positive results for the PVL gene. There was no expression of Sec and CflA between MRSA and MSSA strains. Among MRSA and MSSA isolates, none statistical differences were observed in biofilm production. The analysis of cytokine induction in the inflammatory response of J774 macrophages by MRSA and MSSA isolates did not show statistical difference. Understanding the mechanisms of pathogenesis of S. aureus could provide important clues for both preventing and treating infection caused by these organisms.

KeywordsMRSAMSSAVirulence FactorHospitalar Infection

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Virulence Factors in Methicillin-Resistant <i>Staphylococcus aureus</i> Isolated from ICU Units in Brazil

Abstract

References (34)