Vegetative Development of <i>Sparassis crispa</i> in Various Growth Conditions and Effect of Electric Pulse Simulation on Its Fruit Body Production

Abstract

This study was carried out to detect the favorable mycelial growth conditions and effect of electric pulse stimulation on fruit body production of SC-1 strain of Sparassis crispa . The optimum growth in PDA was found at 25°C (67 mm colony diameter in 28 days) followed by 20°C (63 mm colony diameter in 28 days). There was no mycelial growth at 35°C. The most favourable initial media pH range was found to be 5 - 7 and colony diameter measurements were not statistically different among these pH values (p > 0.05). However, the highest growth was obtained at pH 6 (57 mm colony diameter in 28 days). A basal medium composed of 0.05 g MgSO 4 , 0.46 g KH 2 PO 4 , 1.0 g K 2 HPO 4 , 120 μg thiamine-HCl, 20 g agar and 1000 ml of distilled water was used to investigate growth response of S. crispato different carbon and nitrogen sources. In 28 days, fructose and glucose exhibited best growth (49.4 mm and 31.6 mm colony diameters respectively) and there was no growth on the basal medium supplemented with galactose. Basal medium supplemented with glycine and alanine as nitrogen sources resulted in best growth, 54.4 mm and 50.5 mm colony diameters respectively. There was no mycelial growth in culture medium supplemented with ammonium acetate, ammonium phosphate, arginine and histidine. Electric pulse stimulation improved the fruiting body production. The yields obtained from all bottles in which electric pulse was applied were significantly higher than the yields from the bottles in which electric pulse was not applied. The percent increases of fresh weight yield from control on 100, 120, 130, and 170 kilovolts were 36%, 44%, 75% and 81% respectively. As regard to dry weight yield, the percent increases from control on 100, 120, 130, and 170 kilovolts were 27%, 54%, 63% and 67% respectively.