Molecular Profiles and Antimicrobial Susceptibility of First Isolates of <i>Streptococcus agalactiae</i> Serotype IX in Argentina — Oak Academic Publishing
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Molecular Profiles and Antimicrobial Susceptibility of First Isolates of <i>Streptococcus agalactiae</i> Serotype IX in Argentina
Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
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Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
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Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
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Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
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Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
1 Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
2 Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
3 Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
4 Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
5 Department of Microbiology, Bacteriology, Faculty of Exact, Chemical and Natural Sciences, National University of Misiones, Posadas, Argentina
Streptococcus agalactiae (GBS) is considered as the most important cause of invasive bacterial disease in infants . There are few current data from serotype IX of GBS worldwide. The present work has been done in order to make a contribution to the knowledge of serotype IX through phenotypic and genotypic study of virulence and resistance. Of 200 strains tested, 5.5% were serotype IX and all were colonizing. In 63.6% of the strains the presence of the bac , bca and hylB genes was determined, and in 54.5% of lmb and rib . All strains were susceptible to Clindamycin and Erythromycin however five isolates showed the resistance genes: ermB , ermTR and/or mefA . The presence of serotype IX in Misiones, a province situated in the northeaster of Argentina, which limits with Paraguay and Brazil in South America, gives the region a particular situation. Currently, public health efforts are aimed at prevention and treatment, study of the virulence mechanisms, surveillance of resistance to antibiotics and the development of effective vaccines to prevent GBS infection .
Kilian, M. (2007) Streptococcus and Enterococcus. In: Greenwood, D., Slack, R., Peutherer, J. and Barer, M., Eds., Medical Microbiology, 17th Edition, Elsevier, Amsterdam, 178-193.
Nobbs, A.H., Lamont, R.J. and Jenkinson, H.F. (2009) Streptococcus Adherence and Colonization. Microbiology and Molecular Biology Reviews, 73, 407-450. http://dx.doi.org/10.1128/MMBR.00014-09
Fry, R.M. (1938) Fatal Infections by Haemolytic Streptococcus Group B. The Lancet, 1, 199-201. http://dx.doi.org/10.1016/S0140-6736(00)93202-1
Larsen, J.W. and Sever, J.L. (2008) Group B Streptococcus and Pregnancy: A Review. American Journal of Obstetrics & Gynecology, 198, 448-450. http://dx.doi.org/10.1016/j.ajog.2007.11.030
Lindahl, G., Stalhammar-Carlemalm, M. and Areschoug, T. (2005) Surface Proteins of Streptococcus agalactiae and Related Proteins in Other Bacterial Pathogens. Clinical Microbiology Reviews, 18, 102-127. http://dx.doi.org/10.1128/CMR.18.1.102-127.2005
Oviedo, P., Pegels, E., Laczeski, M., Quiroga, M. and Vergara, M. (2013) Phenotypic and Genotypic Characterization of Streptococcus agalactiae in Pregnant Women. First Study in a Province of Argentina. Brazilian Journal of Microbiology, 44, 253-258. http://dx.doi.org/10.1590/S1517-83822013005000030
Schuchat, A. (1998) Epidemiology of Group B Streptococcal Disease in the United States: Shifting Paradigms. Clinical Microbiology Reviews, 11, 497-413.
Hannoun, A., Shehab, M., Khairallah, M.T., Sabra, A., Abi-Rached, R., Bazi, T., et al. (2009) Correlation between Group B Streptococcal Genotypes, Their Antimicrobial Resistance Profiles, and Virulence Genes among Pregnant Women in Lebanon. International Journal of Microbiology, 2009, 1-6. http://dx.doi.org/10.1155/2009/796512
Zhao, Z., Kong, F., Zeng, X., Gidding, H.F., Morgan, J. and Gilbert, G.L. (2008) Distribution of Genotypes and Antibiotic Resistance Genes among Invasive Streptococcus agalactiae (Group B Streptococcus) Isolates from Australasian Patients Belonging to Different Age Groups. Clinical Microbiology and Infection, 14, 260-267. http://dx.doi.org/10.1111/j.1469-0691.2007.01914.x
Sadowy, E., Matynia, B. and Hryniewicz, W. (2010) Population Structure, Virulence Factors and Resistance Determinants of Invasive, Non-Invasive and Colonizing Streptococcus agalactiae in Poland. Journal of Antimicrobial Chemotherapy, 65, 1907-1914. http://dx.doi.org/10.1093/jac/dkq230
Spellerberg, B. (2000) Pathogenesis of Neonatal Streptococcus agalactiae Infections. Microbes and Infection, 2, 1733-1742. http://dx.doi.org/10.1016/S1286-4579(00)01328-9
Burns, G. and Plumb, J. (2013) GBS Public Awareness, Advocacy, and Prevention—What’s Working, What’s Not and Why We Need a Maternal GBS Vaccine. Vaccine, 31, D58-D65. http://dx.doi.org/10.1016/j.vaccine.2013.02.039
Chen, V.L., Avci, F.Y. and Kasper, D.L. (2013) A Maternal Vaccine against Group B Streptococcus: Past, Present and Future. Vaccine, 31, D13-D19. http://dx.doi.org/10.1016/j.vaccine.2012.12.080
Palmeiro, J.K., Dalla-Costa, L.M., Fracalanzza, S.E., Botelho, A.C., da Silva Nogueira, K., Scheffer, M.C., et al. (2010) Phenotypic and Genotypic Characterization of Group B Streptococcal Isolates in Southern Brazil. Journal of Clinical Microbiology, 48, 4397-4403. http://dx.doi.org/10.1128/JCM.00419-10
Gherardi, G., Imperi, M., Baldassarri, L., Pataracchia, M., Alfarone, G., Recchia, S., et al. (2007) Molecular Epidemiology and Distribution of Serotypes, Surface Proteins, and Antibiotic Resistance among Group B Streptococci in Italy. Journal of Clinical Microbiology, 45, 2909-2916. http://dx.doi.org/10.1128/JCM.00999-07
Laczeski, M., Pegels, E., Oviedo, P., Quiroga, M. and Vergara, M. (2013) Streptococcus agalactiae, First Study in Misiones of Resistance Genes Associated with Capsular Serotypes. Revista de Ciencia y Tecnologia, 19, 66-72.
Clinical and Laboratory Standards Institute (2013) Performance Standards for Antimicrobial Susceptibility Testing. 16th Informational Supplement, 33 (1), M100S23.
Poyart, C., Réglier-Poupet, H., Tazi, A., Billoet, A., Dmytruk, N., Bidet, P., et al. (2008) Invasive Group B Streptococcal Infections in Infants, France. Emerging Infectious Disease, 14, 1647-1649. http://dx.doi.org/10.3201/eid1410.080185
Patten, S., Vollman, A.R., Manning, S.D., Mucenski, M., Vidakovich, J. and Davies, H.D. (2006) Vaccination for Group B Streptococcus during Pregnancy: Attitudes and Concerns of Women and Health Care Providers. Social Science & Medicine, 63, 347-358.
Imperi, M., Pataracchia, M., Alfarone, G., Baldassarri, L., Orefici, G. and Creti, R. (2010) A Multiplex PCR Assay for the Direct Identification of the Capsular Type (Ia to IX) of Streptococcus agalactiae. Journal of Microbiological Methods, 80, 212-214. http://dx.doi.org/10.1016/j.mimet.2009.11.010
Savoia, D., Gottimer, C., Crocilla, C. and Zucca, M. (2008) Streptococcus agalactiae in Pregnant Women: Phenotypic and Genotypic Characters. Journal of Infection, 56, 120-125. http://dx.doi.org/10.1016/j.jinf.2007.11.007
Slotved, H.C., Kong, F., Lambertsen, L., Sauer, S. and Gilbert, G.L. (2007) Serotype IX, a Proposed New Streptococcus agalactiae Serotype. Journal of Clinical Microbiology, 45, 2929-2936. http://dx.doi.org/10.1128/JCM.00117-07
Ueno, H., Yamamoto, Y., Yamamichi, A., Kikuchi, K., Kobori, S. and Miyazaki, M. (2012) Characterization of Group B Streptococcus Isolated from Women in Saitama City, Japan. Japanese Journal of Infectious Diseases, 65, 516-521. http://dx.doi.org/10.7883/yoken.65.516
Ferrieri, P., Lynfield, R., Creti, R. and Flores, A. (2013) Serotype IV and Invasive Group B Streptococcus Disease in Neonates, Minnesota, USA, 2000-2010. Emerging Infectious Diseases, 19, 551-558.
Ippolito, D.L., James, W.A., Tinnemore, D., Huang, R.R., Dehart, M.J., Williams, J., et al. (2010) Group B Streptococcus Serotype Prevalence in Reproductive-Age Women at a Tertiary Care Military Medical Center Relative to Global Serotype Distribution. BMC Infectious Diseases, 10, 336. http://dx.doi.org/10.1186/1471-2334-10-336
Rojo, P., Araya, P., Martinez, T.M.A., Hormazabal, J.C., Maldonado, A. and Fernandez, J. (2008) Molecular Characterization of Chilean Isolates of Streptococcus agalactiae. Revista medica de Chile, 136, 606-612.
Figueroa, J.R., Ibarra, F.J.O., Jaramillo, A.E. and Roman, G.C. (2007) Maternal B Group Streptococcus Colonization in Mexico: Prevalence Based on Literature Review. Ginecología y Obstetricia de Mexico, 75, 399-403.
Paoletti, L.J., Bradford, J. and Paoletti, L.C. (1999) A Serotype VIII Strain among Colonizing Group B Streptococcal Isolates in Boston, Massachusetts. Journal of Clinical Microbiology, 37, 3759-3760.
Quiroga, M., Pegels, E., Oivedo, P., Laczeski, M. and Marta, V. (2011) Antimicrobial Susceptibility of Streptococcus agalactiae Isolated from Pregnant Women in Misiones, Argentina. In: Mendez-Vilas, A., Ed., Science against Microbial Pathogens: Communicating Current Research and Technological Advances, Formatex Research Center, Badajoz, 444-447.
Sambrook, J. and Rusell, D.W. (2001) Molecular Cloning: A Laboratory Manual. Cold Sprong Harbor, New York.
Corso, A., Faccone, D., Gagetti, P., Pace, J., Regueira, M., Pace, J., et al. (2009) Prevalence of mef and ermB Genes in Invasive Pediatric Erythromycin-Resistant Streptococcus pneumoniae Isolates from Argentina. Revista Argentina de Microbiologia, 41, 29-33.
Faccone, D., Lalonardi, F., Abel, S., Machain, M., Errecalde, L., Littvik, A., et al. (2010) Multiple-Clones of Streptococcus agalactiae Harbouring lnuB Gene. Journal of Infection in Developing Countries, 4, 580-582.
Persson, E., Berg, S., Bevanger, L., Bergh, K., Valso-Lyng, R. and Trollfors, B. (2008) Characterisation of Invasive Group B Streptococci Based on Investigation of Surface Proteins and Genes Encoding Surface Proteins. Clinical Microbiology and Infection, 14, 66-73. http://dx.doi.org/10.1111/j.1469-0691.2007.01877.x
Broughton, R.A. and Baker, C.J. (1983) Role of Adherence in the Pathogenesis of Neonatal Group B Streptococcal Infection. Infection and Immunity, 39, 837-843.
Quiroga, M., Pegels, E., Oviedo, P., Pereyra, E. and Vergara, M. (2008) Antibiotic Susceptibility Patterns and Prevalence of Group B Streptococcus Isolated from Pregnant Women in Misiones, Argentina. Brazilian Journal of Microbiology, 39, 245-250. http://dx.doi.org/10.1590/S1517-83822008000200009
Faccone, D., Guerriero, L., Méndez, E., Errecalde, L., Cano, H., Yoyas, N., et al. (2010) Fluoroquinolone-Resistant Streptococcus agalactiae Isolates from Argentina. Revista Argentina de Microbiología, 42, 203-207.
García, S.D., Eliseth, M.C., Lazzo, M.J., Copolillo, E., Barata, A.D., de Torres, R., et al. (2003) Group B Streptococcus Carriers among Pregnant Women. Revista Argentina de Microbiología, 35, 183-187.
Fleming, K.E., Bohnsack, J.F., Palacios, G.C., Takahashi S. and Adderson, E.E. (2004) Equivalence of High-Virulence Clonotypes of Serotype III Group B Streptococcus agalactiae (GBS). Journal of Medical Microbiology, 53, 505-508. http://dx.doi.org/10.1099/jmm.0.05443-0
von Both, U., Ruess, M., Mueller, U., Fluegge, K., Sander, A. and Berner, R. (2003) A Serotype V Clone Is Predominant among Erythromycin-Resistant Streptococcus agalactiae Isolates in a Southwestern Region of Germany. Journal of Clinical Microbiology, 41, 2166-2169. http://dx.doi.org/10.1128/JCM.41.5.2166-2169.2003