Forty seven clinical samples of Fowl adenovirus (FAdV) associated with Inclusion Body Hepatitis (IBH) from Peruvian broilers received between July 2006 and April 2013 were genotyped using sequencing of L1 Loop of Hexon gene. All 47 clinical samples presented macroscopic and histopathology lesions consistent with IBH, and amplified a specific fragment of Hexon gene by Polymerase Chain Reaction (PCR). A unique nucleotide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was obtained in all 47 clinical samples analyzed. This sequence showed a high level of conservation in amino acid and nucleotide sequence (>99%) with a Fowl Adenovirus C serotype 4 previously identified. Sequence and phylogenetic analysis indicate no genotypic variation in Peruvian isolates. The presence of a unique genotype very closely related with genotype C1 previously reported in Peru and Ecuador (>99%), suggests the presence of FAdV C serotype 4 genotype C1 in clinical cases of IBH from Peruvian broilers.
KeywordsFowl Adenovirus CMolecular CharacterizationInclusion Body HepatitisPeru
McFerran, J.B. and Smyth, J.A. (2000) Avian Adenoviruses. Revue Scientifique et Technique, 19, 589-601.
Hess, M. (2000) Detection and Differentiation of Avian Adenoviruses: A Review. Avian Pathology, 29, 195-206. http://dx.doi.org/10.1080/03079450050045440
Nakamura, K., Mase, M., Yamaguchi, S., Shibahara, T. and Yuasa, N. (1999) Pathologic Study of Specific-Pathogen-Free Chicks and Hens Inoculated with Adenovirus Isolated from Hydropericardium Syndrome. Avian Diseases, 43, 414-423. http://dx.doi.org/10.2307/1592638
Toro, H., Prusas, C., Raue, R., Cerda, L., Geisse, C., Gonzalez, C. and Hess, M. (1999) Characterization of Fowl Adenoviruses from Outbreaks of Inclusion Body Hepatitis/Hydropericardium Syndrome in Chile. Avian Diseases, 43, 262-270. http://dx.doi.org/10.2307/1592616
Chandra, R., Shukla, S.K. and Kumar, M. (2000) Thehydropericardium Syndrome and Inclusion Body Hepatitis in Domestic Fowl. Tropical Animal Health and Production, 32, 99-111. http://dx.doi.org/10.1023/A:1005230703093
Ojkic, D., Martin, E., Swinton, J., Vaillancourt, J.P., Boulianne, M. and Gomis, S. (2008) Genotyping of Canadian Isolates of Fowl Adenoviruses. Avian Pathology, 37, 95-100. http://dx.doi.org/10.1080/03079450701805324
Alvarado, I.R., Villegas, P., El-Attrache, J., Jensen, E., Rosales, G., Perozo, F. and Purvis, L.B. (2007) Genetic Characterization, Pathogenicity, and Protection Studies with an Avian Adenovirus Isolate Associated with Inclusion Body Hepatitis. Avian Diseases, 51, 27-32. http://dx.doi.org/10.1637/0005-2086(2007)051[0027:GCPAPS]2.0.CO;2
Raue, R. and Hess, M. (1998) Hexon Based PCRs Combined with Restriction Enzyme Analysis for Rapid Detection and Differentiation of Fowl Adenoviruses and Egg Drop Syndrome Virus. Journal of Virological Methods, 73, 211-217. http://dx.doi.org/10.1016/S0166-0934(98)00065-2
Meulemans, G., Boschmans, M., van den Berg, T.P. and Decaesstecker, M. (2001) Polymerase Chain Reaction Combined with Restriction Enzyme Analysis for Detection and Differentiation of Fowl Adenoviruses. Avian Pathology, 30, 655-660. http://dx.doi.org/10.1080/03079450120092143
Meulemans, G., Couvreur, B., Decaesstecker, M., Boschmans, M. and van den Berg, T.P. (2004) Phylogenetic Analysis of Fowl Adenoviruses. Avian Pathology, 33, 164-170. ttp://dx.doi.org/10.1080/03079450310001652086
Thompson, J.D., Givson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The Clustal_X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 24, 4876-4882. http://dx.doi.org/10.1093/nar/25.24.4876
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739. tp://dx.doi.org/10.1093/molbev/msr121
Kimura, M. (1980) A Simple Method for Estimating Evolutionary Rate of Base Substitutions through Comparative Studies of Nucleotide Sequences. Journal of Molecular Evolution, 16, 111-120. http://dx.doi.org/10.1007/BF01731581
Schwarz, R. and Dayhoff, M. (1979) Matrices for Detecting Distant Relationships. In: Dayhoff, M., Ed., Atlas of Protein Sequences, National Biomedical Research Foundation, 353-358.
Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.
Felsenstein, J. (1985) Confidence Limits on Phylogenies: An Approach Using the Bootstrap. Evolution, 39, 783-791. http://dx.doi.org/10.2307/2408678
Tamura, K., Nei, M. and Kumar, S. (2004) Prospects for Inferring Very Large Phylogenies by Using the Neighbor-Joining Method. Proceedings of the National Academy of Sciences of the United States of America, 101, 11030-11035. http://dx.doi.org/10.1073/pnas.0404206101
Nei, M. and Kumar, S. (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York, 18.
Marek, A., Günes, A., Schulz, E. and Hess, M. (2010) Classification of Fowl Adenoviruses by Use of Phylogenetic Analysis and High-Resolution Melting-Curve Analysis of the Hexon L1 Gene Region. Journal of Virological Methods, 170, 147-154. http://dx.doi.org/10.1016/j.jviromet.2010.09.019
Crawford-Miksza, L. and Shnurr, D.P. (1996) Analysis of 15 Adenovirus Hexon Proteins Reveals the Location and Structure of Seven Hypervariable Regions Containing Serotype-Specific Residues. Journal of Virology, 70, 1836-1844.
Ojkic, D. and Nagy, E. (2000) The Complete Nucleotide Sequence of Fowl Adenovirus Type 8. Journal of General Virology, 81, 1833-1837.
Gaba, A., Parmar, H., Pal, J.K. and Prajapati, K.S. (2010) Isolation, Identification and Molecular Characterization of Inclusion Body Hepatitis Virus. Veterinary World, 3, 415-417.
Parthiban, S., Manoharan, S., Roy, P., Chandranm, N., Aruni, A.W. and Koteeswaran, A. (2005) Nucleotide Sequence Analysis of the L1 Loop Variable Region of Hexon Gene of Fowl Adenovirus 4 Isolates from India. Acta Virologica, 49, 65-68.
Lim, T.H., Lee, H.J., Lee, D.H., Lee, Y.N., Park, J.K., Youn, H.N., Kim, M.S., Youn, H.S., Lee, J.B., Park, S.Y., Choi, I.S. and Song, C.S. (2011) Identification and Virulence Characterization of Fowl Adenoviruses in Korea. Avian Disease, 55, 554-560. http://dx.doi.org/10.1637/9730-032011-Reg.1
Kim, J.N., Byun, S.H., Kim, M.J., Kim, J., Sung, H.W. and Mo, L.P. (2008) Outbreaks of Hydropericardium Syndrome and Molecular Characterization of Korean Fowl Adenoviral Isolates. Avian Disease, 52, 526-530. http://dx.doi.org/10.1637/8178-112207-Case
Hess, M., Raue, R. and Prusas, C. (1999) Epidemiological Studies on Fowl Adenoviruses Isolated from Cases of Infectious Hydropericardium. Avian Pathology, 28, 433-439. http://dx.doi.org/10.1080/03079459994443
Choi, K.S., Kye, S.J., Kim, J.Y., Jeon, W.J., Lee, E.K., Park, K.Y. and Sung, H.W. (2012) Epidemiological Investigation of Outbreaks of Fowl Adenovirus Infection in Commercial Chickens in Korea. Poultry Science, 91, 2502-2506. http://dx.doi.org/10.3382/ps.2012-02296