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Sweet Potato Starch as a Carbon Source for Growth and Glucoamylase Production from <i>Aspergillus niger</i>
Tissue Culture Unit, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI), Umuahia, Nigeria
- 1 Tissue Culture Unit, Biotechnology Research and Development Center, National Root Crops Research Institute (NRCRI), Umuahia, Nigeria
Advances in Microbiology·Volume 04 (2014)·Pages 788–795·Published 15 September 2014·DOI10.4236/aim.2014.412087
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Abstract
A mesophilic strain of Aspergillus niger isolated from cassava effluent samples produced extracellular glucoamylase in submerged culture containing 2% (w/v) soluble or sweet potato starch. On soluble starch medium the maximum glucoamylase activity in the culture filtrate was 9.40 U / mg compared to 8.24 U / mg on sweet potato starch culture filtrate. The mycelial dry weight for both media was 494 and 418 mg respectively. The maximum glucoamylase activity was obtained at a growth temperature of 40 ° C and pH 4.5. The implication is that the bioprocess of utilizing sweet potato starch in the culture is attractive due to its relatively cheaper availability in Nigeria, making it even more favorable when economics is considered.
Keywords<i>Aspergillus niger</i>Sweet Potato StarchSubmerged FermentationAmylolytic ActivityGlucoamylase Prodcution
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