Aim: The present study aims to evaluate the occurrence and characterize E. coli in meat and meat products marketed in Egypt based on their antimicrobial-resistance pattern and production of enterotoxins. Methods: A total of 250 meat samples, categorized as 80 fresh beef, 85 ground beef and 85 beef burger purchased from supermarkets and butchers’ shops were used for isolation of E. coli . All isolates were screened for antimicrobial susceptibility. Plasmid profile analyses were done. Polymerase chain reactions were performed for detection of enterotoxin-encoding genes ( astA, eaeA, stx 1 and stx 2). Results: Twenty-five samples were isolated and identified as E. coli. 14 isolates were multidrug resistant. Plasmids isolation from all isolates revealed that 76% of these isolates harbored plasmids. astA gene was amplified in 7 isolates (28%). Eight (32%) isolates harbored eaeA gene. However, none of the isolates harbored stx 1 or stx 2 genes. Analysis of multiple drug resistant isolates revealed a significant relation between multiple drug resistance and both astA and eaeA. Conclusion: The study confirmed the prevalence of enterotoxin genes ( astA and eaeA ) in E. coli isolated from meat product and the association between the presence of these genes and multiple drug resistant phenomena.
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