Survival, Proliferation and Cell Cycle of Swine Fibroblast after Infection with <i>Salmonella enterica</i> — Oak Academic Publishing
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Survival, Proliferation and Cell Cycle of Swine Fibroblast after Infection with <i>Salmonella enterica</i>
Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
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Department of Immunobiology and Pathogenic Biology, Medical School of Xi’an Jiaotong University, Xi’an, China
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Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
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Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
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Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
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Laboratory of Cell Engineering and Molecular Genetics, Faculty of Science and Engineering, Iwate University, Morioka, Japan
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Department of Clinical Microbiology, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia
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Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
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Laboratory of Nutrition and Life Sciences, Department of Biotechnology and Environmental Engineering, Faculty of Engineering, Hachinohe Institute of Technology, Hachinohe, Japan
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Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
1 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
2 Department of Immunobiology and Pathogenic Biology, Medical School of Xi’an Jiaotong University, Xi’an, China
3 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
4 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
5 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
6 Laboratory of Cell Engineering and Molecular Genetics, Faculty of Science and Engineering, Iwate University, Morioka, Japan
7 Department of Clinical Microbiology, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia
8 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
9 Laboratory of Nutrition and Life Sciences, Department of Biotechnology and Environmental Engineering, Faculty of Engineering, Hachinohe Institute of Technology, Hachinohe, Japan
10 Laboratory of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan
Food-borne salmonellosis continues to be a major health concern worldwide. Carry-contamination of Salmonella frequently occurs in meat production. We focused on cell dynamics of swine fibroblasts after infection with Salmonella enterica serovar Enteritidis and Typhimurium, because fibroblast can be a target cell for Salmonella latent infection. It was found that both S. Enteritidis and S. Typhimurium were able to adhere and invade to swine fibroblasts. The proliferations in fibroblasts were different between each serovar. S. Enteritidis reached to the maximum at 24 hr after infection while S. Typhimurium did not. In addition, the decrease in the G 0 /G 1 phase cells and increase in G 2 /M phase cells on the fibroblast were observed by both Salmonella infection. Cell death including apoptosis in the cells was inhibited by the infection of Salmonella. These results suggest that nontyphoidal Salmonella can survive for the long term by modifying bacterial cell proliferation and preventing cell death of host cells.
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