Age Does Not Affect the Induction of Mortality by the Foodborne Pathogen <i>Salmonella enterica</i> in <i>Caenorhabditis elegans</i> — Oak Academic Publishing
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Age Does Not Affect the Induction of Mortality by the Foodborne Pathogen <i>Salmonella enterica</i> in <i>Caenorhabditis elegans</i>
Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
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Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
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Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
1 Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
2 Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
3 Department of Biological Sciences, Sam Houston State University, Huntsville, TX, USA
Salmonella is a common cause of foodborne illness within the United States with the severity of the infection being a factor of both the age and overall health of the infected individual. The nematode worm Caenorhabditis elegans has proven to be a useful model to study infection dynamics of pathogenic bacteria, including Salmonella enterica , and its short lifespan makes it a powerful model system to assess the effect of organismal age on infection severity. In this study, we infected C. elegans with each of 6 serovars of S. enterica at 1, 3 or 5 days of worm age and monitored their survival. Worms infected with E. coli OP50 were used as a control. Infection with S. enterica resulted in a significant reduction in mean longevity relative to OP50 (p < 0.05); however, there was no significant effect of age on mean survival time regardless of the strain of bacteria used.
Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A., Roy, S.L., Jones, J.L. and Griffin, P.M. (2011) Foodborne Illness Acquired in the United States-Major Pathogens. Emerging Infectious Diseases, 17, 7-15. https://doi.org/10.3201/eid1701.P11101
Ren, Z., Gay, R., Thomas, A., Pae, M., Wu, D., Logsdon, L., Mecsas, J. and Meydani, S.N. (2009) Effect of Age on Susceptibility to Salmonella Typhimurium Infection in C57BL/6 Mice. Journal of Medical Microbiology, 58, 1559-1567. https://doi.org/10.1099/jmm.0.013250-0
Riley, L.W., Cohen, M.L., Seals, J.E., Blaser, M.J., Birkness, K.A., Hargett, N.T., Martin, S.M. and Feldman, R.A. (1984) Importance of Host Factors in Human Salmonellosis Caused by Multiresistant Strains of Salmonella. Journal of Infectious Diseases, 149, 878-883. https://doi.org/10.1093/infdis/149.6.878
Grimont, A. and Weill, F. (2007) Antigenic Formulae of the Salmonella Serovars. 9th ed., Institute Pasteur: World Health Organization Collaborating Center for Reference and Research on Salmonella.
Guibourdenche, M., Roggentin, P., Mikoleit, M., Fields, P.I., Bockemuhl, J., Grimont, P.A. and Weill, F.X. (2010) Supplement 2003-2007 (No. 47) to the White-Kauffmann-Le Minor Scheme. Research in Microbiology, 161, 26-29. https://doi.org/10.1016/j.resmic.2009.10.002
Foley, S.L. and Lynne, A.M. (2008) Food Animal-Associated Salmonella Challenges: Pathogenicity and Antimicrobial Resistance. Journal of Animal Science, 86, E173-E187. https://doi.org/10.2527/jas.2007-0447
Baumler, A.J., Tsolis, R.M., Ficht, T.A. and Adams, L.G. (1998) Evolution of Host Adaptation in Salmonella enterica. Infection and Immunity, 66, 4579-4587.
Brenner, S. (1974) The Genetics of Caenorhabditis elegans. Genetics, 77, 71-94.
Aballay, A. and Ausubel, F.M. (2002) Caenorhabditis elegans as a Host for the Study of Host-Pathogen Interactions. Current Opinion in Microbiology, 5, 97-101. https://doi.org/10.1016/S1369-5274(02)00293-X
Alegado, R.A., Campbell, M.C., Chen, W.C., Slutz, S.S. and Tan, M.W. (2003) Characterization of Mediators of Microbial Virulence and Innate Immunity Using the Caenorhabditis elegans Host-Pathogen Model. Cellular Microbiology, 5, 435-444. https://doi.org/10.1046/j.1462-5822.2003.00287.x
Silverman, G.A., Luke, C.J., Bhatia, S.R., Long, O.S., Vetica, A.C., Perlmutter, D.H. and Pak, S.C. (2009) Modeling Molecular and Cellular Aspects of Human Disease using the Nematode Caenorhabditis elegans. Pediatric Research, 65, 10-18. https://doi.org/10.1203/PDR.0b013e31819009b0
Sifri, C.D., Begun, J. and Ausubel, F.M. (2005) The Worm Has Turned-Microbial Virulence Modeled in Caenorhabditis elegans. Trends in Microbiology, 13, 119-127. https://doi.org/10.1016/j.tim.2005.01.003
Alegado, R.A. and Tan, M.W. (2008) Resistance to Antimicrobial Peptides Contributes to Persistence of Salmonella typhimurium in the C. elegans Intestine. Cellular Microbiology, 10, 1259-1273. https://doi.org/10.1111/j.1462-5822.2008.01124.x
Kim, D.H. and Ausubel, F.M. (2005) Evolutionary Perspectives on Innate Immunity from the Study of Caenorhabditis elegans. Current Opinion in Immunology, 17, 4-10. https://doi.org/10.1016/j.coi.2004.11.007
Aballay, A., Yorgey, P. and Ausubel, F.M. (2000) Salmonella typhimurium Proliferates and Establishes a Persistent Infection in the Intestine of Caenorhabditis elegans. Current Biology, 10, 1539-1542. https://doi.org/10.1016/S0960-9822(00)00830-7
Labrousse, A., Chauvet, S., Couillault, C., Kurz, C.L. and Ewbank, J.J. (2000) Caenorhabditis elegans Is a Model Host for Salmonella typhimurium. Current Biology, 10, 1543-1545. https://doi.org/10.1016/S0960-9822(00)00833-2
Li, Y., Xiang, Q., Zhang, Q., Huang, Y. and Su, Z. (2012) Overview on the Recent Study of Antimicrobial Peptides: Origins, Functions, Relative Mechanisms and Application. Peptides, 37, 207-215. https://doi.org/10.1016/j.peptides.2012.07.001
Portal-Celhay, C., Bradley, E.R. and Blaser, M.J. (2012) Control of Intestinal Bacterial Proliferation in Regulation of Lifespan in Caenorhabditis elegans. BMC Microbiology, 12, 49. https://doi.org/10.1186/1471-2180-12-49
Stiernagle, T. (2006) Maintenance of C. elegans. WormBook, Ed., The C. elegans Research Community, WormBook. http://www.wormbook.org
Hope, I. (1999) C. elegans: A Practical Approach. http://www.wormbook.org/wli/wbg16.2p8/
Cox, D.R. and Oakes, D. (1984) Analysis of Survival Data. Chapman and Hall, London.
Marsh, E.K. and May, R.C. (2012) Caenorhabditis elegans, a Model Organism for Investigating Immunity. Applied and Environmental Microbiology, 78, 2075-2081. https://doi.org/10.1128/AEM.07486-11
Sifri, C.D., Begun, J., Ausubel, F.M. and Calderwood, S.B. (2003) Caenorhabditis elegans as a Model Host for Staphyloccoccus aureus Pathogenesis. Infection and Immunity, 74, 2208-2217. https://doi.org/10.1128/IAI.71.4.2208-2217.2003
Laws, T.R., Harding, S.V., Smith, M.P., Atkins, T.P. and Titball, R.W. (2004) Age Influences Resistance of Caenorhabditis elegans to Killing by Pathogenic Bacteria. FEMS Microbiology Letters, 234, 281-287. https://doi.org/10.1111/j.1574-6968.2004.tb09545.x
Kurz, C.L., Chauvet, S., Andres, E., Aurouze, M., Vallet, I., Michel, G.P., Uh, M., Celli, J., Filloux, A., De Bentzman, S., Steinmentz, I., Hoffman, J.A., Finlay, B.B., Gorvel, J.P., Ferrandon, D. and Ewbank, J.J. (2003) Virulence Factors of the Human Opportunistic Pathogen Serratia marcescens Identified by In Vivo Screening. The EMBO Journal, 22, 1451-1460. https://doi.org/10.1093/emboj/cdg159
Youngman, M.J., Rogers, Z.N. and Kim, D.H. (2011) A Decline in p38 MAPK Signaling Underlies Immunosenescence in Caenorhabditis elegans. PLOS Genetics, 7, e1002082. https://doi.org/10.1371/journal.pgen.1002082
Komura, T., Ikeda, T., Hoshino, K., Shibamura, A. and Nishikawa, Y. (2012) Caenorhabditis elegans as an Alternative Model to Study Senescence of Host Defense and the Prevention by Immunonutrition. Advances in Experimental Medicine and Biology, 710, 19-27. https://doi.org/10.1007/978-1-4419-5638-5_3