Treatment with <i>Enterococcus faecalis</i> CECT7121 Is Not Effective as Therapy in Mice with an Established Allergy Status — Oak Academic Publishing
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Treatment with <i>Enterococcus faecalis</i> CECT7121 Is Not Effective as Therapy in Mice with an Established Allergy Status
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
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Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
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Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
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Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
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Cátedra Microbiología y Parasitología (CUDEMyP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
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Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
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Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
1 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
2 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
3 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
4 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
5 Cátedra Microbiología y Parasitología (CUDEMyP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
6 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
7 Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Instituto de Estudios de la Inmunidad Humoral “Prof. Dr. R.A. Margni” (IDEHU, CONICET-UBA), Buenos Aires, Argentina
In allergies, an unbalanced immune response towards a T helper (Th) 2 profile with high levels of Immunoglobulin (Ig) E is produced. We have demonstrated that the pre-administration of Enterococcus faecalis CECT7121 prevents the development of allergy in ovalbumin-immunized mice. In this work, we evaluated whether this bacterium can also revert an established allergic status. Mice were immunized with ovalbumin (OVA) and after that, were inoculated with an E. faecalis CECT7121 suspension. In immunized animals, serum specific immune response, proliferative activity of memory splenocytes, and levels of Th2 cytokines were assessed. The in vivo active cutaneous anaphylaxis test was also performed. The treatment with E. faecalis CECT7121 only increased anti-OVA IgG2a levels. No differences were observed in other specific immunological parameters. Probiotic-treatment did not prove to have any desensitizing effect on mice. These results, together with those recently published, can be concluded that this bacterium would not be appropriate for the treatment of allergic symptoms.
Wang, C.C. and Rook, G.A.W. (1998) Inhibition of an Established Response to Ovalbumin in BALB/c Mice by Killed Mycobacterium vaccae. Journal of Immunology, 93, 307-313. https://doi.org/10.1046/j.1365-2567.1998.00432.x
Crestani, E., Lohman, C.I., Guerra, S., Wright, A.L. and Halonen, M. (2007) Association of IL-5 Cytokine Production and in vivo IgE Levels in Infants and Parents. Journal of Allergy and Clinical Immunology, 120, 820-826. https://doi.org/10.1016/j.jaci.2007.05.033
Peng, S., Lin, J.Y. and Lin, M.Y. (2007) Antiallergic Effect of Milk Fermented with Lactic Acid Bacteria in a Murine Animal Model. Journal of Agricultural and Food Chemistry, 55, 5092-5096. https://doi.org/10.1021/jf062869s
Nonaka, Y., Izumo, T., Izumi, F., Maekawa, T., Shibata, H., Nakano, A., Kishi, A., Akatani, K. and Kiso, Y. (2008) Antiallergic Effects of Lactobacillus pentosus Strain S-PT84 Mediated by Modulations of Th1/Th2 Immunobalance and Inductions of IL-10 Production. International Archives of Allergy and Immunology, 145, 249-257. https://doi.org/10.1159/000109294
Michail, S. (2009) The Role of Probiotics in Allergic Diseases. Allergy, Asthma & Clinical Immunology, 5, 5-11. https://doi.org/10.1186/1710-1492-5-5
Imada, M., Estelle, F., Simons, R., Jay, F.T. and Hayglass, K.T. (1995) Allergen-Stimulated Interleukin-4 and Interferon-y Production in Primary Culture: Responses of Subjects with Allergic Rhinitis and Normal Controls. Journal of Immunology, 85, 373-380.
Kim, H., Kwack, K., Kim, D.Y. and Ji, G.E. (2005) Oral Probiotic Bacterial Administration Suppressed Allergic Responses in an Ovalbumin-Induced Allergy Mouse Model. FEMS Immunology & Medical Microbiology, 45, 259-267. https://doi.org/10.1016/j.femsim.2005.05.005
Guarner, F., Bourdet-Sicard, R., Brandtzaeg, P., Gill, H.S., McGuirk, P., van Eden, W., Versalovic, J., Weinstock, J.V. and Rook, G.A.W. (2006) Mechanisms of Disease: The Hygiene Hypothesis Revisited. Nature Clinical Practice Gastroenterology & Hepatology, 3, 275-284. https://doi.org/10.1038/ncpgasthep0471
Yeun, K.J., Choi, Y.O. and Ji, G.E. (2008) Effect of Oral Probiotics (Bifidobacterium lactis AD011 and Lactobacillus acidophilus AD031) Administration on Ovalbumin-Induced Food Allergy Mouse Model. Journal of Microbiology and Biotechnology, 18, 1393-1400.
Maldonado-Galdeano, C.M., de Moreno de Le Blanc, A., Vinderola, G., Bonet, M.E. and Perdigón, G. (2007) Proposed Model: Mechanisms of Immunomodulation Induced by Probiotic Bacteria. Clinical and Vaccine Immunology, 14, 485-492. https://doi.org/10.1128/CVI.00406-06
Velez, E.M., Maldonado Galdeano, C., Carmuega, E., Weill, R., Bibas Bonet, M.E. and Perdigón, G. (2011) Probiotic Fermented Milk Consumption Modulates the Allergic Process Induced by Ovoalbumin in Mice. British Journal of Nutrition, 110, 500-508.
Schiavi, E., Barletta, B., Butteroni, C., Corinti, S., Boirivant, M. and Di Felice, G. (2011) Oral Therapeutic Administration of a Probiotic Mixture Suppresses Established Th2 Responses and Systemic Anaphylaxis in a Murine Model of Food Allergy. Allergy, 66, 499-508. https://doi.org/10.1111/j.1398-9995.2010.02501.x
Maldonado-Galdeano, C., Novotny Núnez, I., de Moreno de LeBlanc, A., Carmuega, E., Weill, R. and Perdigón, G. (2011) Impact of a Probiotic Fermented Milk in the Gut Ecosystem and in the Systemic Immunity Using a Non-Severe Protein-Energy-Malnutrition Model in Mice. BMC Gastroenterology, 11, 64-78. https://doi.org/10.1186/1471-230X-11-64
Zhang, J., Su, H., Li, Q., Wu, H., Liu, M., Huang, J., Zeng, M., Zheng, Y. and Sun, X. (2017) Oral Administration of Clostridium butyricum CGMCC0313-1 Inhibits β-Lactoglobulin-Induced Intestinal Anaphylaxis in a Mouse Model of Food Allergy. Gut Pathology, 9, 11-21. https://doi.org/10.1186/s13099-017-0160-6
Yang, B., Xiao, L., Liu, S., Liu, Y., Luo, Y., Ji, Q., Yang, P. and Liu, Z. (2017) Exploration of the Effect of Probiotics Supplementation on Intestinal Microbiota of Food Allergic Mice. American Journal of Translational Research, 9, 376-385.
Moreno, M.F., Sarantinopoulos, P., Tsakalidou, E. and De Vuyst, L. (2006) The Role and Application of Enterococci in Food and Health. International Journal of Food Microbiology, 106, 1-24. https://doi.org/10.1016/j.ijfoodmicro.2005.06.026
Franz, C.M., Huch, M., Abriouel, H., Holzapfel, W. and Gálvez, A. (2011) Enterococci as Probiotics and Their Implications in Food Safety. International Journal of Food Microbiology, 151, 125-140. https://doi.org/10.1016/j.ijfoodmicro.2011.08.014
Sparo, M., Nunez, G.G., Castro, M., Calcagno, M.L., García Allende, M.A., Ceci, M., Najle, R. and Manghi, M. (2008) Characteristics of an Environmental Strain, Enterococcus faecalis CECT7121, and Its Effects as Additive on Craft Dry-Fermented Sausages. Food Microbiology, 25, 607-615. https://doi.org/10.1016/j.fm.2008.01.008
Castro, M., Sparo, M., Molina, M., Andino, J. and Manghi, M. (2007) Enterococcus faecalis CECT7121 Induces Systemic Immunomodulatry Effects and Protects from Salmonella Infection. International Journal of Probiotics and Prebiotics, 2, 215-224.
Castro, M.S., Molina, M.A., Azpiroz, M.B., Díaz, A.M., Ponzio, R., Sparo, M.D., Manghi, M.A. and Canellada, A.M. (2016) Probiotic Activity of Enterococcus faecalis CECT7121: Effects on Mucosal Immunity and Intestinal Epithelial Cells. Journal of Applied Microbiology, 121, 1117-1129. https://doi.org/10.1111/jam.13226
Castro, M., Molina, M., Sparo, M. and Manghi, M. (2008) Effects of Enterococcus faecalis CECT7121 on the Specific Immune Response after DTPw Vaccination. International Journal of Probiotics and Prebiotics, 3, 25-30.
Castro, M.S., Molina, M.A., Di Sciullo, P., Azpiroz, M.B., Leocata Nieto, F., Sterín-Speziale, N.B., Mongini, C. and Manghi, M.A. (2010) Beneficial Activity of Enterococcus faecalis CECT7121 in the Anti-Lymphoma Protective Response. Journal of Applied Microbiology, 109, 1234-1243. https://doi.org/10.1111/j.1365-2672.2010.04747.x
Castro, M.S., Azpiroz, M.B., Molina, M.A., Mourelle, A.C., Alaniz, F.S., Maldonado, A.M. and Manghi, M.A. (2012) Preliminary Studies on the Prevention of the Ovalbumin-Induced Allergic Response by Enterococcus faecalis CECT7121 in Mice. International Archives of Allergy and Immunology, 157, 11-20. https://doi.org/10.1159/000324673
Matsuzaki, T., Yamazaki, R., Hashimoto, S. and Yokokura, T. (1997) Antidiabetic Effect of an Oral Administration of Lactobacillus casei in a Non-Insulin Dependent Diabetes Mellitus (NIDDM) Model Using KK-Ay Mice. Endocrine Journal, 44, 357-365. https://doi.org/10.1507/endocrj.44.357
Kalliomäki, M., Salminen, S., Arvilommi, H., Kero, P., Koskinen, P. and Isolauri, E. (2001) Probiotics in Primary Prevention of Atopic Disease: A Randomized Placebo-Controlled Trial. Lancet, 357, 1076-1079. https://doi.org/10.1016/S0140-6736(00)04259-8
Ishida, Y., Bandou, I., Kanzato, H. and Yamamoto, N. (2003) Decrease in Ovalbumin Specific IgE of Mice Serum after Oral Uptake of Lactic Acid Bacteria. Bioscience, Biotechnology, and Biochemistry, 67, 951-957. https://doi.org/10.1271/bbb.67.951
Hougee, S., Vriesema, A.J.M., Wijering, S.C., Knippels, L.M.J., Folkerts, G., Nijkamp, F.P., Knol, J. and Garssen, J. (2010) Oral Treatment with Probiotics Reduces Allergic Symptoms in Ovalbumin Sensitized Mice: A Bacterial Strain Comparative Study. International Archives of Allergy and Immunology, 151, 107-117. https://doi.org/10.1159/000236000
Walker, W.A. (2014) Intestinal Colonization and Programming of the Intestinal Immune Response. Journal of Clinical Gastroenterology, 4813, S8-S11. https://doi.org/10.1097/MCG.0000000000000230
Gould, H.J., Sutton, B.J., Beavil, A.J., Beavil, R.L., McCloskey, N., Coker, H.A., Fear, D. and Smurthwaite, L. (2003) The Biology of IgE and the Basis of Allergic Disease. Annual Review of Immunology, 21, 579-628. https://doi.org/10.1146/annurev.immunol.21.120601.141103
Levine, S.J., and Wenzel, S.E. (2010) Narrative Review: The Role of Th2 Immune Pathway Modulation in the Treatment of Severe Asthma and Its Phenotypes. Annals of Internal Medicine, 152, 232-237. https://doi.org/10.7326/0003-4819-152-4-201002160-00008
Zuercher, A.W., Weiss, M., Holvoet, S., Moser, M., Moussu, H., van Overtvelt, L., Horiot, S., Moingeon, P., Nutten, S., Prioult, G., Singh A. and Mercenier, A. (2012) Lactococcus lactis NCC 2287 Alleviates Food Allergic Manifestations in Sensitized Mice by Reducing IL-13 Expression Specifically in the Ileum. Clinical & Developmental Immunology, 2012, Article ID: 485750. https://doi.org/10.1155/2012/485750