Analysis of Gut Fungal Community of Cows with Clinical Mastitis

Abstract

Clinical Mastitis (CM) was one of the most common causes leading health disease in cows. In this article, we gave a new insight to gut fungal community of cows with CM. We chose two cows suffering from CM and four healthy cows from a local cow farm. We classified four healthy cows (H1, H2, H3, H4) into the control group and two cows (CM1, CM2) with CM into the case group. High-throughput sequencing was used to detect the difference of fungal community between the case group and the control group. The difference of gut fungi community was detected both at phylum and genus level. 4 phyla and 98 genera have been detected in the control group and the case group. At the phylum level, we found that the relative abundance of Basidiomycota in the case group was lower than that in the control group. At the genus level, the relative abundance of Saccharomycetales -unclassified and Fungi -unclassified were both higher whereas the relative abundance of Pseudallescheria , Trichosporon , Microascaeae -unclassified, Candida and Scedosprium in the case group was lower compared with the healthy group. Totally, the diversity and abundance of gut fungal community in the case group were lower than the control group. In conclusion, there are some differences of gut fungal community between the control group and the case group and the insights from this study could be used to develop a microbiota-based diagnosis for CM.