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<i>E coli</i> Accumulation behind an Obstacle
Lab. de Microscopía Aplicada a Estudios Moleculares y Celulares (LAMAE), Fac. de Ingeniería, Univ. Nac. de Entre Ríos (FI-UNER) and Inst. de Investigación y Desarrollo en Bioingeniería y Bioinformática IBB-(UNER-CONICET), Oro Verde, Argentina
Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Microbiología, Buenos Aires, Argentina
Physique et Mécanique des Milieux Hétérogénes (UMR 7636 ESPCI/CNRS/Univ. P.M. Curie/Univ. Paris-Diderot), Paris, France
Laboratoire FAST, Univ. Paris Sud, CNRS, Université Paris-Saclay, F-91405 Orsay, France
Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
- 1 Lab. de Microscopía Aplicada a Estudios Moleculares y Celulares (LAMAE), Fac. de Ingeniería, Univ. Nac. de Entre Ríos (FI-UNER) and Inst. de Investigación y Desarrollo en Bioingeniería y Bioinformática IBB-(UNER-CONICET), Oro Verde, Argentina
- 2 Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
- 3 Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
- 4 Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Microbiología, Buenos Aires, Argentina
- 5 Physique et Mécanique des Milieux Hétérogénes (UMR 7636 ESPCI/CNRS/Univ. P.M. Curie/Univ. Paris-Diderot), Paris, France
- 6 Laboratoire FAST, Univ. Paris Sud, CNRS, Université Paris-Saclay, F-91405 Orsay, France
- 7 Universidad de Buenos Aires, Facultad de Ingeniería, Grupo de Medios Porosos, Buenos Aires, Argentina
Advances in Microbiology·Volume 08 (2018)·Pages 451–464·Published 12 June 2018·DOI10.4236/aim.2018.86030
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Abstract
This paper describes our findings regarding the accumulation of motile bacteria at the rear of a confined obstacle and the physical description of the mechanisms at play. We found that the modification of flow due to the presence of the obstacle produces vorticity that favor the diffusion of bacteria towards the downstream stagnation point. By testing different flow rates, we determined the range in which bacteria accumulate. More interestingly, we observe that hydrodynamic interaction between the bacteria and the top and bottom surface of the microfluidic chip maintain the bacteria in the region where the flow velocity is lower than their own velocity. In the case of non-motile bacteria, this effect is not observed because bacteria follow the streamlines as passive tracers do.
Keywords<i>E coli</i>MotilityFiltrationMicrofluidicBacterial Accumulation
- Haig, S.J., Collins, G., Davies, R.L., Dorea, C.C. and Quince, C. (2011) Biological Aspects of Slow sand Filtration: Past, Present and Future. Water Science & Technology Water Supply, 11, 468-472. https://doi.org/10.2166/ws.2011.076
- Rippy, M.A. (2015) Meeting the Criteria: Linking Biofilter Design to Fecal Indicator Bacteria Removal. WIREs Water, 2, 577-592. https://doi.org/10.1002/wat2.1096
- Verma, S., Daverey, A. and Sharma, A. (2017) Slow Sand Filtration for Water and Wastewater Treatment—A Review. Environmental Technology Review, 6, 47-58. https://doi.org/10.1080/21622515.2016.1278278
- Ginn, T.R., Wood, B.D., Nelson, K.E., Scheibe, T.D., Murphy, E.M. and Clement, T.P. (2002) Processes in Microbial Transport in the Natural Subsurface. Advances in Water Resources, 25, 1017-1042. https://doi.org/10.1016/S0309-1708(02)00046-5
- Stevik, T.K., Aa, K., Ausland, G. and Hanssen, J.F. (2004) Retention and Removal of Pathogenic Bacteria in Wastewater Percolating through Porous Media: A Review. Water Research, 38, 1355-1367. https://doi.org/10.1016/j.watres.2003.12.024
- Nelson, K.E., Massoudieh, A. and Ginn, T.R. (2007) E. coli Fate and Transport in the Happel Sphere-in-Cell Model. Advances in Water Resources, 30, 1492-1504. https://doi.org/10.1016/j.advwatres.2006.05.027
- Hill, J., Kalkanci, O., McMurry, J.L. and Koser, H. (2007) Hydrodynamic Surface Interactions Enable Escherichia coli to Seek Efficient Routes to Swim Upstream. Physical Review Letters, 98, Article ID: 068101. https://doi.org/10.1103/PhysRevLett.98.068101
- Marcos, Fu, H.C., Powers, T.R. and Stocker, R. (2012) Bacterial Rheotaxis. Proceedings of the National Academy of Sciences of the United States of America, 109, 4780-4785. https://doi.org/10.1073/pnas.1120955109
- Altshuler, E., Miño, G., Pérez-Penichet, C., del Río, L., Lindner, A., Rousselet, A. and Clément, E. (2013) Flow-Controlled Densification and Anomalous Dispersion of E. coli through a Constriction. Soft Matter, 9, 1864-1870. https://doi.org/10.1039/C2SM26460A
- Rusconi, R., Guasto, J.S. and Stocker, R. (2014) Bacterial Transport Suppressed by Fluid Shear. Nature Physics, 10, 212-217. https://doi.org/10.1038/nphys2883
- Figueroa-Morales, N., Miño, G.L., Rivera, A., Caballero, R., Clement, E., Altshuler, E. and Lindner, A. (2015) Living on the Edge: Transfer and Traffic of E. coli in a Confined Flow. Soft Matter, 11, 6284-6293. https://doi.org/10.1039/C5SM00939A