Gastric cancer is one of the most common types of cancer worldwide. Helicobacter pylori is considered one of the most important causes of this condition specially because of its virulence markers as sab A and cag A. Therefore, we aim to investigate the relation between these markers and the gastric diseases in 400 patients who underwent upper digestive endoscopy. To detect the bacteria and its genes by Polymerase Chain Reaction (PCR), the presence of H. pylori was significant when comparing the groups control vs . cancer ( p value vs . chronic gastritis ( p value cag A was statistically significant considering its presence when comparing the chronic gastritis vs . cancer groups ( p value = 0.0434) OR [95% CI] 2.44 (1.021 - 5.845). Associating both sab A and cag A, we found a statistically significant result ( p value vs . cancer groups. Helicobacter pylori is directly associated to gastric diseases such as gastritis and cancer and its virulence markers: sab A and cag A increase the injury process to the gastric epithelium making the host more susceptible to cancer.
Cheung, K.-S. and Leung, W.K. (2018) Risk of Gastric Cancer Development after Eradication of Helicobacter pylori. World Journal of Gastrointestinal Oncology, 10, 115-123. https://doi.org/10.4251/wjgo.v10.i5.115
Zheng, W., Zhang, S., Zhang, S., Min, L., Wang, Y., Xie, J., et al. (2017) The Relationship between Tumor Necrosis Factor-α Polymorphisms and Gastric Cancer Risk: An Updated Meta-Analysis. Biomedical Reports, 7, 133-142. https://doi.org/10.3892/br.2017.934
Xu, Y., Cao, X., Jiang, J., Chen, Y. and Wang, K. (2016) TNF-308/-238 Polymorphisms Areassociated with Gastric Cancer: A Case-Control Family Study in China. Clinics and Research in Hepatology and Gastroenterology, 41, 103-109. https://doi.org/10.1016/j.clinre.2016.05.014
Backert, S. and Tegtmeyer, N. (2017) Type IV Secretion and Signal Transduction of Helicobacter pylori cagA through Interactions with Host Cell Receptors. Toxins, 9, 115. https://doi.org/10.3390/toxins9040115
Gall, A., Gaudet, R.G., Gray-Owen, S.D. and Salama, N.R. (2017) TIFA Signaling in Gastric Epithelial Cells Initiates the Cag Type 4 Secretion System-Dependent Innate Immune Response to Helicobacter pylori Infection. mBio, 8, e01168-17. https://doi.org/10.1128/mBio.01168-17
álvarez-Arellano, L. and Maldonado-Bernal, C. (2014) Helicobacter pylori and Neurological Diseases: Married by the Laws of Inflammation. World Journal of Gastrointestinal Pathophysiology, 5, 400-404. https://doi.org/10.4291/wjgp.v5.i4.400
Klerk, N., Maudsdotter, L., Gebreegziabher, H., Saroj, S.D., Eriksson, B., Eriksson, O.S., et al. (2016) Lactobacilli Reduce Helicobacter pylori Attachment to Host Gastric Epithelial Cells by Inhibiting Adhesion Gene Expression. Infection and Immunity, 84, 1526-1535. https://doi.org/10.1128/IAI.00163-16
Li, M., Zhou, Q., Yang, K., Brigstock, D.R., Zhang, L., Xiu, M., et al. (2015) Rare Case of Helicobacter pylori-Positive Multiorgan IgG4-Related Disease and Gastric Cancer. World Journal of Gastroenterology, 21, 3429-3434. https://doi.org/10.3748/wjg.v21.i11.3429
Magalhães, A., Pinto, R.M., Nairn, A.V., Rosa, M.D., Ferreira, R.M., Neto, S.J., et al. (2015) Helicobacter pylori Chronic Infection and Mucosal Inflammation Switches the Human Gastric Glycosylation Pathways. Biochim Biophys Acta, 1852, 1928-1939. https://doi.org/10.1016/j.bbadis.2015.07.001
Alzahrani, S., Lina, T.T., Gonzalez, J., Pinchuk, I.V., Beswick, E.J. and Reyes, V.E. (2014) Effect of Helicobacter pylori on Gastric Epithelial Cells. World Journal of Gastroenterology, 20, 12767-12780. https://doi.org/10.3748/wjg.v20.i36.12767
Kalali, B., Mejías-Luque, R., Javaheri, A. and Gerhard, M. (2014) H. pylori Virulence Factors: Influence on Immune System and Pathology. Mediators of Inflammation, 2014, Article ID: 426309. https://doi.org/10.1155/2014/426309
Posselt, G., Backert, S. and Wessler, S. (2013) The Functional Interplay of Helicobacter pylori Factors with Gastric Epithelial Cells Induces a Multi-Step Process in Pathogenesis. Cell Communication and Signaling, 11, 77. https://doi.org/10.1186/1478-811X-11-77
Pakbaz, Z., Shirazi, M.H., Ranjbar, R., Reza Pourmand, M., Gholi, M.K., Aliramezani, A., et al. (2013) Frequency of sabA Gene in Helicobacter pylori Strains Isolated From Patients in Tehran, Iran. Iranian Red Crescent Medical Journal, 15, 767-770. https://doi.org/10.5812/ircmj.5044
Santibáñez, M., Aguirre, E., Belda, S., Aragones, N., Saez, J., Rodríguez, J.C., et al. (2015) Relationship between Tobacco, cagA and vacA i1 Virulence Factors and Bacterial Load in Patients Infected by Helicobacter pylori. PLoS ONE, 10, e0126540. https://doi.org/10.1371/journal.pone.0126540
Stolte, M. and Meining, A. (2001) The Updated Sydney System: Classification and Grading of Gastritis as the Basis of Diagnosis and Treatment. Canadian Journal of Gastroenterology, 15, 591-598. https://doi.org/10.1155/2001/367832
Hu, B., El Hajj, N., Sittler, S., Lammert, N., Barnes, R. and Meloni-Ehrig, A. (2012) Gastric Cancer: Classification, Histology and Application of Molecular Pathology. Journal of Gastrointestinal Oncology, 3, 251-261.
Scholte, G.H., van Doorn, L.J., Quint, W.G. and Lindeman, J. (1997) Polymerase Chain Reaction for the Detection of Helicobacter pylori in Formaldehyde-Sublimate Fixed, Paraffin-Embedded Gastric Biopsies. Diagnostic Molecular Pathology, 6, 238-243. https://doi.org/10.1097/00019606-199708000-00008
Pereira, W.N., Ferraz, M.A., Zabaglia, L.M., Labio, R.W., Orcini, W-A., Ximenez, J-P.B., et al. (2014) Association among H. pylori Virulence Markers dupA, cagA and vacA in Brazilian Patients. Journal of Venomous Animals and Toxins Including Tropical Diseases, 20, 1. https://doi.org/10.1186/1678-9199-20-1
Shao, L., Takeda, H., Fukui, T., Mabe, K., Han, J., Kawata, S., et al. (2010) Genetic Diversity of the Helicobacter pylori Sialic Acid-Binding Adhesin (sabA) Gene. BioScience Trends, 4, 249-253.
Rasmussen, L.T., de Labio, R.W., Neto, A.C., Silva, L.C., Queiroz, V.F., Smith, M.A.C., et al. (2012) Detection of Helicobacter pylori in Gastric Biopsies, Saliva and Dental Plaques of Dyspeptic Patients from Marília, São Paulo, Brazil: Presence of vacA and cagA Genes. Journal of Venomous Animals and Toxins Including Tropical Diseases, 18, 180-187. https://doi.org/10.1590/S1678-91992012000200008
Van Doorn, L.J., Figueiredo, C., Rossau, R., Jannes, G., van Asbroek, M., Sousa, J.C., et al. (1998) Typing of Helicobacter pylori vacA Gene and Detection of cagA Gene by PCR and Reverse Hybridization. Journal of Clinical Microbiology, 36, 1271-1276.
Warren, J.R. and Marshall, B. (1983) Unidentified Curved Bacilli on Gastric Epithelium in Active Chronic Gastritis. The Lancet, 321, 1273-1275. https://doi.org/10.1016/S0140-6736(83)92719-8
Suerbaum, S. and Michetti, P. (2002) Helicobacter Pylori Infection. New England Journal of Medicine, 347, 1597-1604. https://doi.org/10.1056/NEJMra020542
Salim, D.K., Sahin, M., Köksoy, S., Adanir, H. and Süleymanlar, I. (2016) Local Immune Response in Helicobacter pylori Infection. Medicine, 95, e3713.
Venerito, M., Vasapolli, R., Rokkas, T., Delchier, J.-C. and Malfertheiner, P. (2017) Helicobacter pylori, Gastric Cancer and Other Gastrointestinal Malignancies. Helicobacter, 22, e12413. https://doi.org/10.1111/hel.12413
Pang, S.S., Nguyen, S.T., Perry, A.J., Day, C.J., Panjikar, S., Tiralongo, J., et al. (2014) The Three-Dimensional Structure of the Extracelular Adhesion Domain of the Sialic Acid-Binding Adhesin SabA from Helicobacter pylori. Journal of Biological Chemistry, 289, 6332-6340. https://doi.org/10.1074/jbc.M113.513135
Oleastro, M. and Ménard, A. (2013) The Role of Helicobacter pylori Outer Membrane Proteins in Adherence and Pathogenesis. Biology, 2, 1110-1134. https://doi.org/10.3390/biology2031110
Nishioka, M., Takeuchi, H., Com, A.S., Uehara, Y., Nishimori, I., Okumiya, T., et al. (2010) The Mechanical Binding Strengths of Helicobacter pylori BabA and SabA Adhesins Using an Adhesion Binding Assay-ELISA, and Its Clinical Relevance in Japan. Microbiology and Immunology, 54, 442-451. https://doi.org/10.1111/j.1348-0421.2010.00237.x
Kato, S., Osaki, T., Kamiya, S., Zhang, X.-S. and Blaser, M.J. (2017) Helicobacter pylori sabA Gene Is Associated with Iron Deficiency Anemia in Childhood and Adolescence. PLoS ONE, 12, e0184046. https://doi.org/10.1371/journal.pone.0184046
Wang, F., Meng, W., Wang, B. and Qiao, L. (2014) Helicobacter pylori-Induced Gastric Inflammation and Gastric Cancer. Cancer Letters, 345, 196-202. https://doi.org/10.1016/j.canlet.2013.08.016
Oldani, A., Cormont, M., Hofman, V., Chiozzi, V., Oregioni, O., Canonici, A., et al. (2009) Helicobacter pylori Counteracts the Apoptotic Action of Its VacA Toxin by Injecting the CagA Protein into Gastric Epithelial Cells. PLoS Pathogens, 5, e1000603. https://doi.org/10.1371/journal.ppat.1000603
Yamaoka, Y., Ojo, O., Fujimoto, S., Odenbreit, S., Haas, R., Gutierrez, O., et al. (2006) Helicobacter pylori Outer Membrane Proteins and Gastroduodenal Disease. Gut, 55, 775-781. https://doi.org/10.1136/gut.2005.083014
Backert, S., Clyne, M. and Tegtmeyer, N. (2011) Molecular Mechanisms of Gastric Epithelial Cell Adhesion and Injection of CagA by Helicobacter pylori. Cell Communication and Signaling, 9, 28. https://doi.org/10.1186/1478-811X-9-28