Effects of Different Broth Enrichment upon Phage Magnetoelastic Biosensor for Fast Detecting Low <i>Salmonella</i> Counts on Problematic Produce — Oak Academic Publishing
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Effects of Different Broth Enrichment upon Phage Magnetoelastic Biosensor for Fast Detecting Low <i>Salmonella</i> Counts on Problematic Produce
Material Research and Education Center, Auburn University, Auburn, AL, USA
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Material Research and Education Center, Auburn University, Auburn, AL, USA
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Material Research and Education Center, Auburn University, Auburn, AL, USA
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Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, China
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Jiangsu Key Laboratory of Materials Surface Technology, School of Materials Science and Engineering, Changzhou University, Changzhou, China
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Material Research and Education Center, Auburn University, Auburn, AL, USA
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Department of Poultry Science, Auburn University, Auburn, AL, USA
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Material Research and Education Center, Auburn University, Auburn, AL, USA
1 Material Research and Education Center, Auburn University, Auburn, AL, USA
2 Material Research and Education Center, Auburn University, Auburn, AL, USA
3 Material Research and Education Center, Auburn University, Auburn, AL, USA
4 Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, China
5 Jiangsu Key Laboratory of Materials Surface Technology, School of Materials Science and Engineering, Changzhou University, Changzhou, China
6 Material Research and Education Center, Auburn University, Auburn, AL, USA
7 Department of Poultry Science, Auburn University, Auburn, AL, USA
8 Material Research and Education Center, Auburn University, Auburn, AL, USA
According to the FDA Bacteriological Analytical Manual (BAM) for Salmonella identification in produce, two pre-enrichment steps with 48 hours of incubation are the golden procedures. Lactose broth is recommended for the first pre-enrichment step medium for leafy greens, and the universal pre-enrichment (UP) broth is for tomatoes. However, the suggested broths were evaluated to have the maximum performance using the culture-dependent methods, and may not be applied to other methods, such as biosensor detection platform. A wireless bacteriophage magnetoelastic (ME) biosensor has been recently developed for real-time or rapid detection of food-borne pathogens in various foods. This affinity-based biosensor utilizes a phage oligonucleotide as the probe to capture target bacteria. In this study, the efficiencies of different pre-enrichment media for early detection of low Salmonella on spinach leaves and tomatoes use ME biosensors to shorten detection time. Four broths of modified peptone water, Lennox broth (LB), lactose broth, and UP broth were selected in this study. Various pre-enrichment times for ME biosensor detection were investigated. After spiking 4 cfu/g Salmonella on the tomatoes surfaces, the phage biosensor was able to detect Salmonella within 5 hours of pre-enrichment comparing to 24 hours in the FDA procedures. For Salmonella spiked spinach leaves, the same medium showed Salmonella positive within 7 hours. This study demonstrated that LB broth is the best medium to shorten pre-enrichment time to pass Salmonella number detection thresholds for ME biosensor detection in spinach and tomatoes when comparing to FDA procedures.
Painter, J.A., Hoekstra, R.M., Ayers, T., Tauxe, R.V., Braden, C.R., Angulo, F.J. and Griffin, P.M. (2013) Attribution of Foodborne Illnesses, Hospitalizations, and Deaths to Food Commodities by Using Outbreak Data, United States, 1998-2008. Emerging Infectious Diseases, 19, 407-415. https://doi.org/10.3201/eid1903.111866
Jacobsen, C.S. and Bech, T.B. (2012) Soil Survival of Salmonella and Transfer to Freshwater and to Fresh Produce. Food Research International, 45, 557-566. https://doi.org/10.1016/j.foodres.2011.07.026
Sivapalasingam, S., Friedman, C.R., Cohen, L. and Tauxe, R.V. (2004) Fresh Produce: A Growing Cause of Outbreaks of Foodborne Illness in the United States, 1973 through 1997. Journal of Food Protection, 67, 2342-2352. https://doi.org/10.4315/0362-028X-67.10.2342
Center for Disease Control and Prevention (CDC). https://www.cdc.gov/salmonella/adelaide-06-18/index.html
FDA Bacteriological Analytical Manual, Chapter 5 for Salmonella, Updated 08/2016. http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149.htm
Bell, R.L., Jarvis, K.G., Ottesen, A.R. and McFarland, M.A. (2016) Brown E.W. Recent and Emerging Innovations in Salmonella Detection: A Food and Environmental Perspective. Microbial Biotech, 9, 279-292. https://doi.org/10.1111/1751-7915.12359
FDA Bacteriological Analytical Manual, Appendix 1. BAM: Rapid Methods for Detecting Foodborne Pathogens. http://www.fda.gov/downloads/Food/FoodScienceResearch/UCM244777.pdf
Guo, X., Chen, J., Beuchat, L.R. and Brackett, R.E. (2000) PCR Detection of Salmonella enterica Serotype Montevideo in and on Raw Tomatoes Using Primers Derived from hilA. Applied and Environmental Microbiology, 66, 5248-5252. https://doi.org/10.1128/AEM.66.12.5248-5252.2000
Ogunjimi, A.A. and Choudary, P.V. (1999) Adsorption of Endogenous Polyphenols Relieves the Inhibition by Fruit Juices and Fresh Produce of Immuno-PCR Detection of Escherichia coli O157:H7. FEMS Immunology and Medical Microbiology, 23, 213-220. https://doi.org/10.1016/S0928-8244(98)00138-2
Guntupalli, R., Hu, J., Lakshmanan, R.S., Huang, T.S., Barbaree, J.M. and Chin, B.A. (2007) A Magnetoelastic Resonance Biosensor Immobilized with Polyclonal Antibody for the Detection of Salmonella typhimurium. Biosensors and Bioelectronics, 22, 1474-1479. https://doi.org/10.1016/j.bios.2006.06.037
Nanduri, V., Sorokulova, I.B., Samoylov, A.M., Simonian, A.L., Petrenko, V.A. and Vodyanoy, V. (2007) Phage as a Molecular Recognition Element in Biosensors Immobilized by Physical Adsorption. Biosensors and Bioelectronics, 22, 986-992. https://doi.org/10.1016/j.bios.2006.03.025
Zourob, M. and Ripp, S. (2007) Bacteriophage-Based Biosensors. In: Zourob, M., Ed., Recognition Receptors in Biosensors, Springer, New York, 415-448. https://doi.org/10.1007/978-1-4419-0919-0_11
Cinti, S., Volpe, G., Piermarini, S., Delibato, E. and Palleschi, G. (2017) Electrochemical Biosensors for Rapid Detection of Foodborne Salmonella: A Critical Overview. Sensors, 17, 1910. https://doi.org/10.3390/s17081910
Li, S., Li, Y., Chen, I.H., Horikawa, S., Shen, W., Simonian, A. and Chin, B.A. (2010) Direct Detection of Salmonella typhimurium on Fresh Produce Using Phage-Based Magnetoelastic Biosensors. Biosensors and Bioelectronics, 26, 1313-1319. https://doi.org/10.1016/j.bios.2010.07.029
Chai, Y., Wikle, H.C., Wang, Z., Horikawa, S., Best, S., Cheng, Z., Dyer, D.F. and Chin, B.A. (2013) Design of a Surface-Scanning Coil Detector for Direct Bacteria Detection on Food Surfaces Using a Magnetoelastic Biosensor. Journal of Applied Physics, 114, Article ID: 104504. https://doi.org/10.1063/1.4821025
Horikawa, S., Chai, Y., Wikle, H.C., Suh, S., Barbaree, J.M. and Chin, B.A. (2015) Direct Detection of Salmonella on Fresh Produce. ECS Transactions, 69, 25-31. https://doi.org/10.1149/06938.0025ecst
Lakshmanan, R.S., Guntupalli, R., Hu, J., Kim, D.J., Petrenko, V.A., Barbaree, J.M. and Chin, B.A. (2007) Phage Immobilized Magnetoelastic Sensor for the Detection of Salmonella typhimurium. Journal of Microbiological Methods, 71, 55-60. https://doi.org/10.1016/j.mimet.2007.07.012
Huang, S., Yang, H., Lakshmanan, R.S., Johnson, M.L., Wan, J., Chen, I.-H., Wikle, H.C., Petrenko, V.A., Barbaree, J.M. and Chin, B.A. (2009) Sequential Detection of Salmonella typhimurium and Bacillus anthracis Spore Using Magnetoelastic Biosensors. Biosensors and Bioelectronics, 24, 1730-1736. https://doi.org/10.1016/j.bios.2008.09.006
Du, S., Horikawa, S., Liu, Y., Chai, Y., Hu, J., Wang, F. and Chin, B.A. (2015) Rapid Detection of Pathogens by a 3D Biomolecular Filter and Automated Biosensor Measurement System. ECS Transactions, 69, 17-24. https://doi.org/10.1149/06938.0017ecst
Chen, I.-H., Horikawa, S., Bryant, K., Riggs, R., Chin, B.A. and Barbaree, J.M. (2017) Bacterial Assessment of Phage Magnetoelastic Biosensor for Salmonella enterica Typhimurium Detection in Chicken Meat. Food Control, 71, 273-278. https://doi.org/10.1016/j.foodcont.2016.07.003
Rose, B.E. (1998) Isolation and Identification of Salmonella from Meat, Poultry and Egg Products. In: Dey, B.P. and Lattuada, C.P., Eds., USDA/FSIS Microbiology Laboratory Guidebook, 3rd Edition, U.S. Department of Agriculture, Food Safety and Inspection Services, Washington DC, 4.1-4.14.
Wang, F., Horikawa, S., Hu, J., Wikle, H.C., Chen, I.-H., Du, S., Liu, Y. and Chin, A.B. (2017) Detection of Salmonella typhimurium on Spinach Using Phage-Based Magnetoelastic Biosensors. Sensors, 17, pii: E386. https://doi.org/10.3390/s17020386
Wang, H., Gill, V.S., Cheng, C.M., Gonzalez-Escalona, N., Irvin, K.A., Zheng, J., Bell, R.L., Jacobson, A.P. and Hammack, T.S. (2015) Evaluation and Comparison of Rapid Methods for the Detection of Salmonella in Naturally Contaminated Pine Nuts Using Different Pre-Enrichment Media. Food Microbiology, 46, 58-65. https://doi.org/10.1016/j.fm.2014.06.028
Qiang, X., Sun, K., Xing, L., Xu, Y., Wang, H., Zhou, Z.P., Zhang, J., Zhang, F., Caliskan, B., Wang, M. and Qiu, Z. (2017) Discovery of a Polystyrene Binding Peptide Isolated from Phage Display Library and Its Application in Peptide Immobilization. Scientific Reports, 7, Article No. 2673. https://doi.org/10.1038/s41598-017-02891-x
Jacobson, A.P., Wang, H., Gill, V.S., Duvall, R., Arce, G., Chirtel, S. and Hammock, T.S. (2017) Relative Effectiveness of Selected Preenrichment Media for the Detection of Salmonella from Leafy Green Produce and Herbs. Food Microbiology, 63, 123-128. https://doi.org/10.1016/j.fm.2016.11.006
González-Escalona, N., Hammack, T.S., Russell, M., Jacobson, A.P., De Jesús, A.J., Brown, E.W. and Lampel, K.A. (2009) Detection of Live Salmonella sp. Cells in Produce by a TaqMan-Based Quantitative Reverse Transcriptase Real-Time PCR Targeting invA mRNA. Applied and Environmental Microbiology, 75, 3714-3720. https://doi.org/10.1128/AEM.02686-08