Gut microbiota plays a major role in regulating the host metabolism and immune system. However, the structure of microbiome population is altered constantly by diverse factors including diet and environment. In particular, the gut microbiome dynamics is influenced by diet composition and their associated metabolites. Many studies in the recent past reported on diet induced dysbiosis in the gut microbiome, the modulating ratio between Firmicutes and Bacteroidetes plays a central role in maintaining the microbiome diversity in the gut and their abundance regulates obese conditions. Although there are several reports on gut microbial dysbiosis ( Firmicutes / Bacteroidetes ) at phylum level but only few of them highlight at the genera level. In the present study, we focused more on the impact of cafeteria diet (CAF) with respect to the gut microbiome richness at the genera level in SD rats. Three weeks old Sprague Dawley (SD) rats were fed with normal chow diet and cafeteria diet (CAF). After 10 weeks, serum, tissue samples (small intestine and cecum), cecum fecal and fecal pellet were collected. Biochemical analysis from serum, Gene expression analysis of pro-inflammatory markers from tissues and microbiome analysis from fecal samples were analysed. CAF diet fed rat in the present study developed obesity with increased body weight, few of them developed the resistance to weight gain (WGR) and these animal shows significantly increased abundance of Bacteriodetes-Prevotella compared to obese animals. This study suggests that detailed research needed to address the contribution of microbiota abundance at the genera level. We further explored the influence of diet induced microbiota changes on immune response; increase in Bacteroidetes leads to increased LPS and this in turn elicit immune reaction in the gut. Moreover, variation in gut microbiota also affects the gut barrier function (Tight junction proteins) and increase metabolic endotoxemia that leads to activation of innate immune system and low-grade inflammation. Further studies on these lines targeting the microbiota changes in the gut at the genera level is warranted to gain more knowledge.
Rowland, I., Gibson, G., Heinken, A., Scott, K., Swann, J., Thiele, I. and Tuohy, K. (2018) Gut Microbiota Functions: Metabolism of Nutrients and Other Food Components. European Journal of Nutrition, 57, 1-24. https://doi.org/10.1007/s00394-017-1445-8
Do, M., Lee, E., Oh, M.J., Kim, Y. and Park, H.Y. (2018) High-Glucose or -Fructose Diet Cause Changes of the Gut Microbiota and Metabolic Disorders in Mice without Body Weight Change. Nutrients, 10, 761. https://doi.org/10.3390/nu10060761
Milagro, F.I., Campión, J. and Martínez, J.A. (2006) Weight Gain Induced by High- Fat Feeding Involves Increased Liver Oxidative Stress. Obesity, 14, 1118-1123. https://doi.org/10.1038/oby.2006.128
Rainone, V., Schneider, L., Saulle, I., Ricci, C., Biasin, M., Al-Daghri, N.M., Giani, E., Zuccotti, G.V., Clerici, M. and Trabattoni, D. (2016) Upregulation of Inflammasome Activity and Increased Gut Permeability Are Associated with Obesity in Children and Adolescents. International Journal of Obesity, 40, 1026. https://doi.org/10.1038/ijo.2016.26
Gregor, M.F. and Hotamisligil, G.S. (2011) Inflammatory Mechanisms in Obesity. Annual Review of Immunology, 29, 415-445. https://doi.org/10.1146/annurev-immunol-031210-101322
Varadharajan, K., Shanmugakonar, M., Das, S.C. and Al-Naemi, H.A. (2018) Gut Microbiota Dysbiosis in Cafeteria Diet Fed Sprague Dawley Rats. Advances in Microbiology, 8, 975-993. https://doi.org/10.4236/aim.2018.812066
Marques, C., Meireles, M., Norberto, S., Leite, J., Freitas, J., Pestana, D., Faria, A. and Calhau, C. (2016) High-Fat Diet-Induced Obesity Rat Model: A Comparison between Wistar and Sprague-Dawley Rat. Adipocyte, 5, 11-21. https://doi.org/10.1080/21623945.2015.1061723
Buyukdere, Y., Gulec, A. and Akyol, A. (2019) Cafeteria Diet Increased Adiposity in Comparison to High Fat Diet in Young Male Rats. PeerJ, 7, e6656. https://doi.org/10.7717/peerj.6656
Scoaris, C.R., Rizo, G.V., Roldi, L.P., de Moraes, S.M.F., de Proença, A.R.G., Peralta, R.M. and Natali, M.R.M. (2010) Effects of Cafeteria Diet on the Jejunum in Sedentary and Physically Trained Rats. Nutrition, 26, 312-320. https://doi.org/10.1016/j.nut.2009.04.012
Sampey, B.P., Vanhoose, A.M., Winfield, H.M., Freemerman, A.J., Muehlbauer, M.J., Fueger, P.T., Newgard, C.B. and Makowski, L. (2011) Cafeteria Diet Is a Robust Model of Human Metabolic Syndrome with Liver and Adipose Inflammation: Comparison to High-Fat Diet. Obesity, 19, 1109-1117. https://doi.org/10.1038/oby.2011.18
de Castro Ghizoni, C.V., Gasparin, F.R.S., Júnior, A.S.M., Carreno, F.O., Constantin, R.P., Bracht, A., Iwamoto, E.L.I. and Constantin, J. (2013) Catabolism of Amino Acids in Livers from Cafeteria-Fed Rats. Molecular and Cellular Biochemistry, 373, 265-277. https://doi.org/10.1007/s11010-012-1499-0
Gasparin, F.R.S., Carreño, F.O., Mewes, J.M., Gilglioni, E.H., Pagadigorria, C.L.S., Natali, M.R.M., Utsunomiya, K.S., Constantin, R.P., Ouchida, A.T., Curti, C. and Gaemers, I.C. (2018) Sex Differences in the Development of Hepatic Steatosis in Cafeteria Diet-Induced Obesity in Young Mice. Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease, 1864, 2495-2509. https://doi.org/10.1016/j.bbadis.2018.04.004
Heitmann, B.L. and Frederiksen, P. (2009) Thigh Circumference and Risk of Heart Disease and Premature Death: Prospective Cohort Study. British Medical Journal, 339, 3292. https://doi.org/10.1136/bmj.b3292
Buyukdere, Y., Gulec, A. and Mutlu, A.A. (2018) Effect of Cafeteria Diet and High Fat Diet on Body Composition and Biochemical Parameters in Rats. Clinical Nutrition, 37, S269-S270. https://doi.org/10.1016/j.clnu.2018.06.1947
MacQueen, H.A., Sadler, D.A., Moore, S.A., Daya, S., Brown, J.Y., Shuker, D.E., Seaman, M. and Wassif, W.S. (2007) Deleterious Effects of a Cafeteria Diet on the Livers of Nonobese Rats. Nutrition Research, 27, 38-47. https://doi.org/10.1016/j.nutres.2006.10.003
Mariné-Casadó, R., Domenech-Coca, C., Del Bas, J.M., Bladé, C., Arola, L. and Caimari, A. (2018) Intake of an Obesogenic Cafeteria Diet Affects Body Weight, Feeding Behavior and Glucose and Lipid Metabolism in a Photoperiod-Dependent Manner in F344 Rats. Frontiers in Physiology, 9, 639. https://doi.org/10.3389/fphys.2018.01639
Brooks, S.P., McAllister, M., Sandoz, M. and Kalmokoff, M.L. (2003) Culture-In- dependent Phylogenetic Analysis of the Faecal Flora of the Rat. Canadian Journal of Microbiology, 49, 589-601. https://doi.org/10.1139/w03-075
Turnbaugh, P.J., Ley, R.E., Mahowald, M.A., Magrini, V., Mardis, E.R. and Gordon, J.I. (2006) An Obesity-Associated Gut Microbiome with Increased Capacity for Energy Harvest. Nature, 444, 1027. https://doi.org/10.1038/nature05414
Krajmalnik-Brown, R., Ilhan, Z.E., Kang, D.W. and DiBaise, J.K. (2012) Effects of Gut Microbes on Nutrient Absorption and Energy Regulation. Nutrition in Clinical Practice, 27, 201-214. https://doi.org/10.1177/0884533611436116
Murphy, E.A., Velazquez, K.T. and Herbert, K.M. (2015) Influence of High-Fat-Diet on Gut Microbiota: A Driving Force for Chronic Disease Risk. Current Opinion in Clinical Nutrition and Metabolic Care, 18, 515. https://doi.org/10.1097/MCO.0000000000000209
Magnusson, K.R., Hauck, L., Jeffrey, B.M., Elias, V., Humphrey, A., Nath, R., Perrone, A. and Bermudez, L.E. (2015) Relationships between Diet-Related Changes in the Gut Microbiome and Cognitive Flexibility. Neuroscience, 300, 128-140. https://doi.org/10.1016/j.neuroscience.2015.05.016
Collado, M.C., Isolauri, E., Laitinen, K. and Salminen, S. (2008) Distinct Composition of Gut Microbiota during Pregnancy in Overweight and Normal-Weight Women. The American Journal of Clinical Nutrition, 88, 894-899. https://doi.org/10.1093/ajcn/88.4.894
Schwiertz, A., Taras, D., Schäfer, K., Beijer, S., Bos, N.A., Donus, C. and Hardt, P.D. (2010) Microbiota and SCFA in Lean and Overweight Healthy Subjects. Obesity, 18, 190-195. https://doi.org/10.1038/oby.2009.167
Zuo, H.J., Xie, Z.M., Zhang, W.W., Li, Y.R., Wang, W., Ding, X.B. and Pei, X.F. (2011) Gut Bacteria Alteration in Obese People and Its Relationship with Gene Polymorphism. World Journal of Gastroenterology: WJG, 17, 1076-1081. https://doi.org/10.3748/wjg.v17.i8.1076
Zhang, H., DiBaise, J.K., Zuccolo, A., Kudrna, D., Braidotti, M., Yu, Y., Parameswaran, P., Crowell, M.D., Wing, R., Rittmann, B.E. and Krajmalnik-Brown, R. (2009) Human Gut Microbiota in Obesity and after Gastric Bypass. Proceedings of the National Academy of Sciences, 106, 2365-2370. https://doi.org/10.1073/pnas.0812600106
Koliada, A., Syzenko, G., Moseiko, V., Budovska, L., Puchkov, K., Perederiy, V., Gavalko, Y., Dorofeyev, A., Romanenko, M., Tkach, S. and Sineok, L. (2017) Association between Body Mass Index and Firmicutes/Bacteroidetes Ratio in an Adult Ukrainian Population. BMC Microbiology, 17, 120. https://doi.org/10.1186/s12866-017-1027-1
Duncan, S.H., Lobley, G.E., Holtrop, G., Ince, J., Johnstone, A.M., Louis, P. and Flint, H.J. (2008) Human Colonic Microbiota Associated with Diet, Obesity and Weight Loss. International Journal of Obesity, 32, 1720-1724. https://doi.org/10.1038/ijo.2008.155
Huttenhower, C., Gevers, D., Knight, R., Abubucker, S., Badger, J.H., Chinwalla, A.T., Creasy, H.H., Earl, A.M., FitzGerald, M.G., Fulton, R.S. and Giglio, M.G. (2012) Structure, Function and Diversity of the Healthy Human Microbiome. Nature, 486, 207-214. https://doi.org/10.1038/nature11234