Determination of <i>α</i>-1,3-Linked Mannose Residue in the Cell Wall Mannan of <i>Candida tropicalis</i> NBRC 1400 Strain — Oak Academic Publishing
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Determination of <i>α</i>-1,3-Linked Mannose Residue in the Cell Wall Mannan of <i>Candida tropicalis</i> NBRC 1400 Strain
Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
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Sanko Medical Tomato Pharmacy Co. Ltd., Shimogumigo, Kawatanacho, Nagasaki, Japan
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Sasebo City General Hospital, Hirasecho, Sasebo, Nagasaki, Japan
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Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
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Divisions of Infection Control and Prevention, Department of Pharmaceutical Science, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
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Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
1 Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
2 Sanko Medical Tomato Pharmacy Co. Ltd., Shimogumigo, Kawatanacho, Nagasaki, Japan
3 Sasebo City General Hospital, Hirasecho, Sasebo, Nagasaki, Japan
4 Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
5 Divisions of Infection Control and Prevention, Department of Pharmaceutical Science, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
6 Divisions of Microbiology, Nagasaki International University, Huis Ten Bosch, Sasebo, Nagasaki, Japan
To investigate the chemical structure of cell wall mannan obtained from pathogenic yeast, Candida tropicalis NBRC 1400 (former antigenic standard strain, IFO 1400). As a result of two-dimensional NMR analysis, it was shown that the mannan of this strain is composed of α -1,6-, α -1,3-, α -1,2- and β -1,2-linked mannose residues. In this research, the mannan was subjected to three degradation procedures, acid-treatment, α -mannosidase, and acetolysis under two conditions in order to determine the chemical structure of the antigenic oligomannosyl side chains in this molecule. The 1 H-nuclear magnetic resonance spectra of resultant oligosaccharides, pentaose and hexaose, demonstrated the existence of the oligomannosyl side chains corresponding to Man α 1-3Man α 1-2Man α 1-2Man α 1-2Man and Man α 1-3Man α 1-2Man α 1-2Man α 1-2Man α 1-2Man, respectively, which have previously also been found in Candida albicans serotype A strain mannans. These findings indicate that C. tropicalis and C. albicans serotype A have no significant difference in the chemical structure of these cell wall mannans. Therefore, it can be interpreted that it is extremely difficult to distinguish both species by targeting the antigenic group in these mannans.
Keywords<i>Candida tropicalis</i>Pathogenic YeastCell Wall MannanAntigenic Oligomannosyl Side ChainAcetolysis<i>α</i>-13-Linked Mannose Residue
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