Extraction of Fat and Fatty Acid Composition from Slaughterhouse Waste by Evaluating Conventional Analytical Methods — Oak Academic Publishing
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Extraction of Fat and Fatty Acid Composition from Slaughterhouse Waste by Evaluating Conventional Analytical Methods
National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
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National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
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Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
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Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
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National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
1 National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
2 National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
3 Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
4 Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
5 National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan
To attain maximum recovery of useful compounds from slaughterhouse waste (Suet, Tongue, Pancreas) of selected ruminant (cow, goat, lamb, and bull), the fat extraction efficiency of popular methods was compared along with fatty acid (FAs) composition. Four selected methods including Soxhlet (SOX), acid hydrolysis, Bligh & dyer (B&D), and Folch (FOL) were assessed. After methylation, extracted lipids were analyzed by Gas chromatography for FA composition. Data indicated that all selected methods were significantly (p < 0.05) different from each other, particularly higher differences were noticed for low lipid-containing products (Tongue, Pancreas) as well as their respective FA Composition. Based on Analysis of Variance and Principal component analysis, the effective method for lipid and FA Composition analysis was the FOL method. The Soxhlet method was only effective for samples with high-fat content i.e., suet, while the B&D method gave comparatively low lipid content in analyzed samples. Hence based on the results, excellent fat and fatty acid extraction was achieved with the FOL method.
Heinz, G. and Hautzinger, P. (2007) Meat Processing Technology for Small-To Medium-Scale Producers (RAP Publication 2007). Food and Agriculture Organization of the United Nations (FAO), Bangkok.
Bah, C.S.F., Bekhit, A.E.-D.A., Carne, A. and McConnell, M.A. (2013) Slaughterhouse Blood: An Emerging Source of Bioactive Compounds. Comprehensive Reviews in Food Science and Food Safety, 12, 314-331. https://doi.org/10.1111/1541-4337.12013
FAO, F.O. (2017) Biannual Report on Global Food Markets. Food and Agriculture Organization of the United Nations, Rome, 1-142.
Walsh, C. (2014) The Use of Animal By-Products—AHDB Beef & Lamb. EBLEX, 2014, Article ID: 130514.
Alfaia, C.M., Alves, S.P., Pestana, J.M., Madeira, M.S., Moreira, O., Santos-Silva, J., Bessa, R.J., Toldrá, F. and Prates, J.A. (2017) Distinct Fatty Acid Composition of Some Edible By-Products from Bovines Fed High or Low Silage Diets. Food Science and Technology International, 23, 209-221. https://doi.org/10.1177/1082013216674137
Hejnfelt, A. and Angelidaki, I. (2009) Anaerobic Digestion of Slaughterhouse By-Products. Biomass and Bioenergy, 33, 1046-1054. https://doi.org/10.1016/j.biombioe.2009.03.004
Palatsi, J., Viñas, M., Guivernau, M., Fernandez, B. and Flotats, X. (2011) Anaerobic Digestion of Slaughterhouse Waste: Main Process Limitations and Microbial Community Interactions. Bioresource Technology, 102, 2219-2227. https://doi.org/10.1016/j.biortech.2010.09.121
Rajagopal, R., Massé, D.I. and Singh, G. (2013) A Critical Review on Inhibition of Anaerobic Digestion Process by Excess Ammonia. Bioresource Technology, 143, 632-641. https://doi.org/10.1016/j.biortech.2013.06.030
Salminen, E. and Rintala, J. (2002) Anaerobic Digestion of Organic Solid Poultry Slaughterhouse Waste—A Review. Bioresource Technology, 83, 13-26. https://doi.org/10.1016/S0960-8524(01)00199-7
Lynch, S.A., Mullen, A.M., O’Neill, E.E. and García, C.á. (2017) Harnessing the Potential of Blood Proteins as Functional Ingredients: A Review of the State of the Art in Blood Processing. Comprehensive Reviews in Food Science and Food Safety, 16, 330-344. https://doi.org/10.1111/1541-4337.12254
Lasekan, A., Abu Bakar, F. and Hashim, D. (2013) Potential of Chicken By-Products as Sources of Useful Biological Resources. Waste Management, 33, 552-565. https://doi.org/10.1016/j.wasman.2012.08.001
Markit, I. (2018) Fats and Oils Industry Overview.
Iverson, S.J., Lang, S.L.C. and Cooper, M.H. (2001) Comparison of the Bligh and Dyer and Folch Methods for Total Lipid Determination in a Broad Range of Marine Tissue. Lipids, 36, 1283-1287. https://doi.org/10.1007/s11745-001-0843-0
Silva, P.T., Detmann, E., Filho, S.C.V., Detmann, K.S.C., Barros, L.V., Martins, S.C.V., Morais, L.E. and Costa, V.A.C. (2011) Evaluation of Total and Non-Fatty Ether Extract in Feeds and Cattle Feces Using Two Analytical Methods. Animal Feed Science and Technology, 163, 111-117. https://doi.org/10.1016/j.anifeedsci.2010.10.012
Adhikari, B.B., Chae, M. and Bressler, D.C. (2018) Utilization of Slaughterhouse Waste in Value-Added Applications: Recent Advances in the Development of Wood Adhesives. Polymers, 10, 176. https://doi.org/10.3390/polym10020176
Talpur, F.N., Bhanger, M.I. and Khuhawar, M.Y. (2007) Intramuscular Fatty Acid Profile of Longissimus Dorsi and Semitendinosus Muscle from Kundi Steers Fed on Pasture with Cottonseed Cake Supplement. International Journal of Food Science & Technology, 42, 1007-1011. https://doi.org/10.1111/j.1365-2621.2006.01451.x
AOAC (2002) Fat Content. In: Official Methods of Analysis, Method 960.39, 17th Edition, Association Official Analytical Chemists, Gaithersburg.
AOAC (2006) Official Methods of Analysis Lipids, Fats and Oils Analysis Total Fat by Acid Hydrolysis Pet Food—Item 24. 17th Edition, Association of Analytical Communities, Gaithersburg, Reference Data: Method 954.02 (4.5.02 or 7.063); NFNAP; LIPD; FA.
Habeck, S., Mitchell, B. and Sullivan, D. (2013) Comparison of Fat Extraction Methods for Analysis of Meat. Proceedings AOAC 127th Annual Meeting & Exposition, Chicago, 25-28 August 2013.
Bligh, E.G. and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification. Canadian Journal of Biochemistry and Physiology, 37, 911-917. https://doi.org/10.1139/o59-099
Folch, J., Lees, M. and Sloane Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues. Journal of Biological Chemistry, 226, 497-509. https://doi.org/10.1016/S0021-9258(18)64849-5
David, F., Sandra, P. and Wylie, P.L. (2002) Improving the Analysis of Fatty Acid Methyl Esters Using Retention Time Locked Methods and Retention Time Databases. Agilent Technologies—Application.
USDA (2018) National Nutrient Database for Standard Reference Legacy Release. 133339, Beef, Variety Meats and By-Products, Tongue, Raw. https://fdc.nal.usda.gov/fdc-app.html#/food-details/170196/nutrients
USDA (2018) National Nutrient Database for Standard Reference Legacy Release. 17220, Lamb, Variety Meats and By-Products, Tongue, Raw. https://fdc.nal.usda.gov/fdc-app.html#/food-details/174366/nutrients
USDA (2018) National Nutrient Database for Standard Reference Legacy Release. 13331, Beef, Variety Meats and By-Products, Pancreas, Raw. https://fdc.nal.usda.gov/fdc-app.html#/food-details/169452/nutrients
USDA (2018) National Nutrient Database for Standard Reference Legacy Release. 17210, Lamb, Variety Meats and By-Products, Pancreas, Raw. https://fdc.nal.usda.gov/fdc-app.html#/food-details/172538/nutrients
USDA (2018) National Nutrient Database for Standard Reference Legacy Release. 13335, Beef, Variety Meats and By-Products, Suet, Raw. https://fdc.nal.usda.gov/fdc-app.html#/food-details/170193/nutrients
Sündermann, A., Eggers, L.F. and Schwudke, D. (2016) Liquid Extraction: Bligh and Dyer. In: Wenk, M.R., Ed., Encyclopedia of Lipidomics, Springer, Dor-drecht, 1-4. https://doi.org/10.1007/978-94-007-7864-1_88-1
Danlami Jibrin, M., Arsad, A., Ahmad Zaini Muhammad, A. and Sulaiman, H. (2014) A Comparative Study of Various Oil Extraction Techniques from Plants. Reviews in Chemical Engineering, 30, 605-626. https://doi.org/10.1515/revce-2013-0038
Brooks, S.P.J., Ratnayake, W.M.N., Lampi, B.J. and Hollywood, R. (1998) Measuring Total Lipid Content in Rat Carcasses: A Comparison of Commonly Employed Extraction Methods. Journal of Agricultural and Food Chemistry, 46, 4214-4217. https://doi.org/10.1021/jf980052w
Pérez-Palacios, T., Ruiz, J., Martín, D., Muriel, E. and Antequera, T. (2008) Comparison of Different Methods for Total Lipid Quantification in Meat and Meat Products. Food Chemistry, 110, 1025-1029. https://doi.org/10.1016/j.foodchem.2008.03.026
Carta, G., Murru, E., Banni, S. and Manca, C. (2017) Palmitic Acid: Physiological Role, Metabolism and Nutritional Implications. Frontiers in Physiology, 8, 902. https://doi.org/10.3389/fphys.2017.00902
Choulis, N.H. (2011) Chapter 49—Miscellaneous Drugs, Materials, Medical Devices and Techniques. In: Aronson, J.K., Ed., Side Effects of Drugs Annual, Elsevier, Amsterdam, 1009-1029. https://doi.org/10.1016/B978-0-444-53741-6.00049-0
Hiremath, P., Nuguru, K. and Agrahari, V. (2019) Chapter 8—Material Attributes and Their Impact on Wet Granulation Process Performance. In: Narang, A.S. and Badawy, S.I.F., Eds., Handbook of Pharmaceutical Wet Granulation, Academic Press, Cambridge, 263-315. https://doi.org/10.1016/B978-0-12-810460-6.00012-9
Xiao, L., Mjøs, S.A. and Haugsgjerd, B.O. (2012) Efficiencies of Three Common Lipid Extraction Methods Evaluated by Calculating Mass Balances of the Fatty Acids. Journal of Food Composition and Analysis, 25, 198-207. https://doi.org/10.1016/j.jfca.2011.08.003
Rader, J.I., Angyal, G., O’Dell, R.G., Weaver, C.M., Sheppard, A.J. and Bueno, M.P. (1995) Determination of Total Fat and Saturated Fat in Foods by Packed Column Gas-Liquid Chromatography after Acid Hydrolysis. Food Chemistry, 54, 419-427. https://doi.org/10.1016/0308-8146(95)00054-M
Wiseman, J., Powles, J. and Salvador, F. (1998) Comparison between Pigs and Poultry in the Prediction of the Dietary Energy Value of Fats. Animal Feed Science and Technology, 71, 1-9. https://doi.org/10.1016/S0377-8401(97)00142-9
Aksnes, A. and Opstvedt, J. (1998) Content of Digestible Energy in Fish Feed Ingredients Determined by the Ingredient-Substitution Method. Aquaculture, 161, 45-53. https://doi.org/10.1016/S0044-8486(97)00255-X
Smit, L.E., Schönfeldt, H.C. and Beer, W.H.J.D. (2004) Comparison of the Energy Values of Different Dairy Products Obtained by Various Methods. Journal of Food Composition and Analysis, 17, 361-370. https://doi.org/10.1016/j.jfca.2004.02.006