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Determination of Voriconazole in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry: Application in Therapeutic Drug Monitoring

¹ Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
² Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
³ Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
⁴ Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
⁵ Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
⁶ Department of Pharmaceutical Analysis, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia
⁷ Research Office, King Abdullah International Medical Research Center, King Saud bin Abdulaziz University for Health Sciences, Ministry of the National Guard—Health Affairs, Riyadh, Saudi Arabia

American Journal of Analytical Chemistry·Volume 14 (2023)·Pages 378–389·Published 21 September 2023·DOI10.4236/ajac.2023.149021

Abstract

A sensitive, accurate and robust Liquid Chromatography Tandem Mass Spectrometry method has been developed and validated to measure voriconazole trough levels in human plasma. The plasma samples were mixed with fluconazole as an Internal Standard and directed to protein precipitation and drug extraction. An aliquot of 1 μl was injected into the chromatographic system and separated by the Acquity BEH C18 column at a flow rate of 0.30 ml/min in a gradient mobile phase consisting of acetonitrile, Ultrapure water (UPW), methanol and formic acid. Voriconazole was detected by a Triple Quadrupole Detector (TQD) operating on Multiple Reaction Monitoring (MRM) and a positive ion mode Electrospray ionization (ESI) Q1 mass: 350.1 m/z, Q3 mass: 281.1 m/z. Method linearity of the calibration curve (0.10 - 8.00 μg/ml) indicated a correlation coefficient r ≥ 0.99. The intra and inter-assay accuracy was within 85% - 115% and the intra and inter-assay precision was ≤5.76%. Voriconazole recovery percentage was between 97.69 - 119.62%. The method was successively applied in routine voriconazole TDM.

KeywordsVoriconazoleHuman PlasmaLiquid ChromatographyTandem Mass SpectrometryTherapeutic Drug Monitoring

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Determination of Voriconazole in Human Plasma by Liquid Chromatography Tandem Mass Spectrometry: Application in Therapeutic Drug Monitoring

Abstract

References (28)