This study presents an optimization of the Folin-Ciocalteu spectrophotometric method for the determination of total phenol content. Multivariate optimization using factorial planning 2 2 with a central point and central composite planning was constructed to evaluate the influence of variables in the process and maximize radiation absorption with minimal radiation scattering caused by solid formation. X-ray fluorescence and X-ray diffraction spectrometry were used to evaluate the chemical composition of solids formed and nephelometric and spectrophotometric studies were also used to evaluate whether the type, origin, dilution and dry extract contents of commercial propolis extracts would significantly influence the increase in radiation scattering and absorption. The optimized methodology added several advantages, such as reduction of reagents, time analysis, and higher accuracy.
Ignat, I., Volf, I. and Popa, V.I. (2011) A Critical Review of Methods for Characterisation of Polyphenolic Compounds in Fruits and Vegetables. Food Chemistry , 126, 1821-1835. https://doi.org/10.1016/j.foodchem.2010.12.026
Robards, K. and Antolovich, M. (1997) Analytical Chemistry of Fruit Bioflavonoidsa Review. The Analyst , 122, 11R-34R. https://doi.org/10.1039/a606499j
Singleton, V.L. and Rossi, J.A. (1965) Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture , 16, 144-158. https://doi.org/10.5344/ajev.1965.16.3.144
Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M. (1999) Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology , Elsevier, 299, 152-178. https://doi.org/10.1016/s0076-6879(99)99017-1
Roginsky, V. and Lissi, E. (2005) Review of Methods to Determine Chain-Breaking Antioxidant Activity in Food. Food Chemistry , 92, 235-254. https://doi.org/10.1016/j.foodchem.2004.08.004
Folin, O. and Ciocalteu, V. (1927) On Tyrosine and Tryptophane Determinations in Proteins. Journal of Biological Chemistry , 73, 627-650. https://doi.org/10.1016/s0021-9258(18)84277-6
Everette, J.D., Bryant, Q.M., Green, A.M., Abbey, Y.A., Wangila, G.W. and Walker, R.B. (2010) Thorough Study of Reactivity of Various Compound Classes toward the Folin-Ciocalteu Reagent. Journal of Agricultural and Food Chemistry , 58, 8139-8144. https://doi.org/10.1021/jf1005935
Cunha, I.B.S., Sawaya, A.C.H.F., Caetano, F.M., Shimizu, M.T., Marcucci, M.C., Drezza, F.T., et al . (2004) Factors That Influence the Yield and Composition of Brazilian Propolis Extracts. Journal of the Brazilian Chemical Society , 15, 964-970. https://doi.org/10.1590/s0103-50532004000600026
Box, J.D. (1983) Investigation of the Folin-Ciocalteau Phenol Reagent for the Determination of Polyphenolic Substances in Natural Waters. Water Research , 17, 511-525. https://doi.org/10.1016/0043-1354(83)90111-2
Lowry, O., Rosebrough, N., Farr, A.L. and Randall, R. (1951) Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry , 193, 265-275. https://doi.org/10.1016/s0021-9258(19)52451-6
Folin, O. and Denis, W. (1912) On Phosphotungstic-Phosphomolybdic Compounds as Color Reagents. Journal of Biological Chemistry , 12, 239-243. https://doi.org/10.1016/s0021-9258(18)88697-5
Swain, T. and Hillis, W.E. (1959) The Phenolic Constituents of Prunus domestica . I.—The Quantitative Analysis of Phenolic Constituents. Journal of the Science of Food and Agriculture , 10, 63-68. https://doi.org/10.1002/jsfa.2740100110
Peterson, G.L. (1979) Review of the Folin Phenol Protein Quantitation Method of Lowry, Rosebrough, Farr and Randall. Analytical Biochemistry , 100, 201-220. https://doi.org/10.1016/0003-2697(79)90222-7
Popova, M., Bankova, V., Butovska, D., Petkov, V., Nikolova‐Damyanova, B., Sabatini, A.G., et al . (2004) Validated Methods for the Quantification of Biologically Active Constituents of Poplar‐Type Propolis. Phytochemical Analysis , 15, 235-240. https://doi.org/10.1002/pca.777
Teixeira, É.W., Message, D., Negri, G., Salatino, A. and Stringheta, P.C. (2007) Seasonal Variation, Chemical Composition and Antioxidant Activity of Brazilian Propolis Samples. Evidence - Based Complementary and Alternative Medicine , 7, 307-315. https://doi.org/10.1093/ecam/nem177
Sulaiman, G.M., Sammarrae, K.W.A., Ad’hiah, A.H., Zucchetti, M., Frapolli, R., Bello, E., et al . (2011) Chemical Characterization of Iraqi Propolis Samples and Assessing Their Antioxidant Potentials. Food and Chemical Toxicology , 49, 2415-2421. https://doi.org/10.1016/j.fct.2011.06.060
Rebiai, A., Lanez, T. and Belfar, M.L. (2010) In vitro Evaluation of Antioxidant Capacity of Algerian Propolis by Spectrophotometrical and Electrochemical Assays. International Journal of Pharmacology , 7, 113-118. https://doi.org/10.3923/ijp.2011.113.118
Al Naggar, Y., Sun, J., Robertson, A., Giesy, J.P. and Wiseman, S. (2016) Chemical Characterization and Antioxidant Properties of Canadian Propolis. Journal of Apicultural Research , 55, 305-314. https://doi.org/10.1080/00218839.2016.1233700
Salgueiro, F.B. and Castro, R.N. (2016) Comparação entre a composição química e capacidade antioxidante de diferentes extratos de própolis verde. Quimica Nova , 39, 1192-1199.
Pazin, W.M., da Mata Mônaco, L., Egea Soares, A.E., Miguel, F.G., Berretta, A.A. and Ito, A.S. (2017) Antioxidant Activities of Three Stingless Bee Propolis and Green Propolis Types. Journal of Apicultural Research , 56, 40-49. https://doi.org/10.1080/00218839.2016.1263496
Ebadi, P. and Fazeli, M. (2017) Anti-Photoaging Potential of Propolis Extract in UVB-Irradiated Human Dermal Fibroblasts through Increasing the Expression of FOXO3A and NGF Genes. Biomedicine & Pharmacotherapy , 95, 47-54. https://doi.org/10.1016/j.biopha.2017.08.019
Molnár, S., Mikuska, K., Patonay, K., Sisa, K., Daood, H.G., Némedi, E., et al . (2017) Comparative Studies on Polyphenolic Profile and Antimicrobial Activity of Propolis Samples Selected from Distinctive Geographical Areas of Hungary. Food Science and Technology International , 23, 349-357. https://doi.org/10.1177/1082013217697469
Hernández Zarate, M.S., Abraham Juárez, M.R., Cerón García, A., Ozuna López, C., Gutiérrez Chávez, A.J., Segoviano Garfias, J.J.N., et al . (2018) Flavonoids, Phenolic Content, and Antioxidant Activity of Propolis from Various Areas of Guanajuato, Mexico. Food Science and Technology , 38, 210-215. https://doi.org/10.1590/fst.29916
Christina, D., Hermansyah, H., Wijanarko, A., Rohmatin, E., Sahlan, M., Pratami, D.K., et al . (2018). Selection of Propolis Tetragonula s p . Extract Solvent with Flavonoids and Polyphenols Concentration and Antioxidant Activity Parameters. AIP Conference Proceedings , 1933, Article 030020. https://doi.org/10.1063/1.5023967
Escriche, I. and Juan-Borrás, M. (2018) Standardizing the Analysis of Phenolic Profile in Propolis. Food Research International , 106, 834-841. https://doi.org/10.1016/j.foodres.2018.01.055
Sowmya, S., Gujjari, A.K., Mruthunjaya, K., et al . (2019) Antioxidant and Antimicrobial Activity of Propolis. Journal of Evolution of Medical and Dental Sciences , 8, 152-154. https://doi.org/10.14260/jemds/2019/33
Pobiega, K., Kraśniewska, K., Derewiaka, D. and Gniewosz, M. (2019) Comparison of the Antimicrobial Activity of Propolis Extracts Obtained by Means of Various Extraction Methods. Journal of Food Science and Technology , 56, 5386-5395. https://doi.org/10.1007/s13197-019-04009-9
Aghaabbasi, K., Askari, N., Hassani Kumleh, H., Torkzadeh-Mahani, M. and Ramzani-Ghara, A. (2019) The Blepharis Persica Seed Hydroalcoholic Extract Synergistically Enhances the Apoptotic Effect of Doxorubicin in Human Colon Cancer and Gastric Cancer Cells. Molecular Biology Reports , 47, 843-853. https://doi.org/10.1007/s11033-019-04711-z
M. Afonso, A., Gonçalves, J., Luís, Â., Gallardo, E. and Duarte, A.P. (2020) Evaluation of the in vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey. Applied Sciences , 10, Article 1845. https://doi.org/10.3390/app10051845
Asem, N., Abdul Gapar, N.A., Abd Hapit, N.H. and Omar, E.A. (2019) Correlation between Total Phenolic and Flavonoid Contents with Antioxidant Activity of Malaysian Stingless Bee Propolis Extract. Journal of Apicultural Research , 59, 437-442. https://doi.org/10.1080/00218839.2019.1684050
Mahamat, A.A., Nyemb, J.N., Gade, I.S., Ngenge, A.T., Talla, E., Céline, H., et al . (2020) A New Fatty Acid and Some Triterpenoids from Propolis of Nkambe (North-West Region, Cameroon) and Evaluation of the Antiradical Scavenging Activity of Their Extracts. Open Chemistry , 18, 239-243. https://doi.org/10.1515/chem-2020-0016
Dalponte Dallabona, I., de Lima, G.G., Cestaro, B.I., Tasso, I.S., Paiva, T.S., Laureanti, E.J.G., et al . (2020) Development of Alginate Beads with Encapsulated Jabuticaba Peel and Propolis Extracts to Achieve a New Natural Colorant Antioxidant Additive. International Journal of Biological Macromolecules , 163, 1421-1432. https://doi.org/10.1016/j.ijbiomac.2020.07.256
Silici, S. and Baysa, M. (2020) Antioxidant, Antiradical and Antipyretic Effects of Olive Oil Extract of Propolis. Journal of Apicultural Research , 59, 883-889. https://doi.org/10.1080/00218839.2020.1753916
Hamilton, W.C. (1964) Statistics in Physical Science: Estimation, Hypothesis Testing, and Least Squares. Ronald Press.
Guide, EURACHEM-CITAC (2000) Quantifying Uncertainty in Analytical Measurement. Laboratory of the Government Chemist, London.
DQO-CGRE-008 (2020) Orientação sobre validação de métodos analíticos. Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (INMETRO).
Joint Committee on Powder Diffraction Standards (1972) Index to the (Inorganic) Powder Diffraction File.
Barbeira, P.J.S., Paganotti, R.S.N. and Ássimos, A.A. (2013) Development of a Multivariate Calibration Model for the Determination of Dry Extract Content in Brazilian Commercial Bee Propolis Extracts through UV-Vis Spectroscopy. Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy , 114, 441-448. https://doi.org/10.1016/j.saa.2013.05.009
Ministério da Agricultura, Pecuária e do Abastecimento (2001) Regulamento da Identidade e Qualidade de Extrato de Própolis, BRASIL, Instrução normativa No. 3.
Luo, C., Zou, X., Li, Y., Sun, C., Jiang, Y. and Wu, Z. (2011) Determination of Flavonoids in Propolis-Rich Functional Foods by Reversed Phase High Performance Liquid Chromatography with Diode Array Detection. Food Chemistry , 127, 314-320. https://doi.org/10.1016/j.foodchem.2011.01.006
Pellati, F., Orlandini, G., Pinetti, D. and Benvenuti, S. (2011) HPLC-DAD and HPLC-ESI-MS/MS Methods for Metabolite Profiling of Propolis Extracts. Journal of Pharmaceutical and Biomedical Analysis , 55, 934-948. https://doi.org/10.1016/j.jpba.2011.03.024