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Chiral Method Development Using Polysaccharide-Based Chiral Stationary Phase (CSP) and Investigation of Chiral Column Degradation and Regeneration
Neurocrine Biosciences INC., San Diego, CA, USA
Neurocrine Biosciences INC., San Diego, CA, USA
Mirati Therapeutics, San Diego, CA, USA
Neurocrine Biosciences INC., San Diego, CA, USA
- 1 Neurocrine Biosciences INC., San Diego, CA, USA
- 2 Neurocrine Biosciences INC., San Diego, CA, USA
- 3 Mirati Therapeutics, San Diego, CA, USA
- 4 Neurocrine Biosciences INC., San Diego, CA, USA
American Journal of Analytical Chemistry·Volume 15 (2024)·Pages 395–406·Published 5 December 2024·DOI10.4236/ajac.2024.1512026
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Abstract
Two polysaccharide-based chiral stationary phase columns were evaluated to improve the previous partial chiral peak separation to a baseline-resolved separation of the INGREZZA® drug substance and its diastereomers. Moreover, the tailing factor (Tf) variation was studied to investigate chiral column degradation and regeneration and to optimize chiral column performance and efficiency.
KeywordsChiral Reversed-Phase HPLC SeparationBackflushChiral Column RegenerationINGREZZA®(Valbenazine)
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