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Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization
Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
Faculdade de Americana, Americana, Brazil
Programa de Pós-gradua??o em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
- 1 Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
- 2 Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
- 3 Faculdade de Americana, Americana, Brazil
- 4 Programa de Pós-gradua??o em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil
American Journal of Analytical Chemistry·Volume 03 (2012)·Pages 113–117·Published 22 February 2012·DOI10.4236/ajac.2012.32016
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Abstract
An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.
KeywordsGlutamineFood Additive ProductLiquid Chromatography
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