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Simple Spectrophotometric Sequential Injection Analysis System for Determination of Serum Calcium
Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
- 1 Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
- 2 Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
- 3 Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
- 4 Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand
American Journal of Analytical Chemistry·Volume 03 (2012)·Pages 131–137·Published 22 February 2012·DOI10.4236/ajac.2012.32019
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Abstract
A sequential injection analysis (SIA) system for the determination of total calcium in blood has been implemented. The assay principle is based on the arsenazo III method, in which the indicator’s complex with calcium forms a blue-purple colour. The absorbance peak height at 650 nm was proportional to the calcium content in samples. A linear range was obtained from 2.5 - 15 mg?dL–1 (r2 = 0.999), with a detection limit of 0.430 mg?dL–1 (3σ). Magnesium, glucose, hae-moglobin, and bilirubin at 123, 1,000, 50, and 0.5 mg?dL–1, respectively, were not found to interfere with the proposed system significantly (recovery 99.1% - 104.4%). The sampling frequency was 30 h–1, in which the carry-over effect was negligible (0.69%). The proposed SIA system was successfully applied to the determination of calcium from serum samples (r2 = 0.970; n = 30).
KeywordsSequential Injection Analysis SystemCalciumArsenazo IIIGreen ChemistrySpectrophotometryClinical Analysis
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