A 100% Water Mobile Phase HPLC-PDA Analysis of Meamine and Related Analogues
- 1 Graduate School of Human Life Science, Osaka City University, Osaka, Japan
Abstract
This paper describes a reserved-phase high performance liquid chromatographic method for detecting melamine (MEL) and related analogues, cyanuric acid (CYA), ammeline (AML), and ammelide (AMD), using a 100% water mobile phase. Chromatographic separation was performed an Inertsil? ODS-4 (250 × 4.6 mm, 5 μm) with a water mobile phase and a photodiode-array detector. The monitoring wavelength was adjusted to 210 nm which represents an average maximum for all the analytes. The total run time was < 8 min. The method shows high stability, significant linearity and satisfactory sensitivity. The detection limits were established in the range 23 - 46 ng.mL–1. An inexpensive and harmless method for the simultaneous detection of MEL, CYA, AML, and AMD was developed and may be further applied to the quantification in foods.
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