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An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment
Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia.
Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia
School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia.
Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
- 1 Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
- 2 Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
- 3 School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia.
- 4 Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia
- 5 School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia.
- 6 Industrial Biotechnology Research Centre, SIRIM Berhad, Shah Alam, Malaysia.
American Journal of Analytical Chemistry·Volume 03 (2012)·Pages 364–370·Published 29 May 2012·DOI10.4236/ajac.2012.35048
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Abstract
A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of gluta-mate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 – 70 μM (R2 = 0.9955) with detection limit of 11 μM. A good agreement (R2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.
KeywordsOptical BiosensorGlutamate DehydrogenaseDiaphoraseAmmonium
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