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A Simple Colorimetric Method for the Evaluation of Chitosan
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American Journal of Analytical Chemistry·Volume 01 (2010)·Pages 91–94·Published 14 September 2010·DOI10.4236/ajac.2010.12012
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Abstract
A simple sensitive and rapid colorimetric method has been developed, and herein described, for the qualitative and quantitative chemical assessment of the commercially available chitosan products. The described method relies on the reactivity of the basic amino function of chitosan with the acid dye bromocresol purple. The applied technique allows assessment of variability and selectivity changes in the quality of the marketed chitosan products.
KeywordsChitosanDyeColorimetricBromocresol Purple
- M. N. V. R. Kumar, “A Review of Chitin and Chitosan Applications,” Reactive and Functional Polymers, Vol. 46, No. 1, 2000, pp. 1-27.
- G. A. F. Roberts, “Thirty Years of Progress in Chitin and Chitosan,” Progress on Chemistry and Application of Chitin, Vol. 13, 2008.
- S.-O. Fernandez-Kim, “Physicochemical and Functional Properties of Crawfish Chitosan as Affected by Different Processing Protocols,” M. S. Dissertation, Seoul National University, Seoul, 2004.
- N. I. Larionova, D. K. Zubaerova, D. T. Guranda, M. A. Pechyonkin and N.G. Balabushevich, “Colorimetric Assay of Chitosan in Presence of Proteins and Polyelectrolytes by Using O-Phthalaldehyde,” Carbohydrate Polymers, Vol. 75, No. 4, 2009, pp. 724-727.
- J. J. Park, “Development of Biomems Device and Package for a Spatially Programmable Biomolecule Assembly,” Ph.D. Dissertation, Faculty of the Graduate School of the University of Maryland, College Park, 2006.
- K. van de Velde and P. Kiekens, “Structure Analysis and Degree of Substitution of Chitin, Chitosan and Dibutyryl Chitin by FT-IR spectroscopy and Solid State 13C NMR,” Carbohydrate Polymers, Vol. 58, No. 4, 1998, p. 409.
- R. A. A. Muzzarelli, “Colorimetric Determination of Chitosan,” Analytical Biochemistry, No. 260, 1998, pp. 255-257.
- C. Wischke and H. Borchert, “Increased Sensitivity of Chitosan Determination by a Dye Binding Method,” Carbohydrate Research, Vol. 341, No. 18, 2006, pp. 2978-2979.
- T. A. Khan, K. K.Peh and H. S. Ch’ng, “Reporting Degree of Deacetylation Values of Chitosan: The Influence of Analytical Methods,” Journal of Pharmacy & Pharmaceutical Sciences, Vol. 5, No. 3, 2002, pp. 205-212.
- K. M. Picker-Freyer and D. Brink, “Evaluation of Powder and Tableting Properties of Chitosan,” AAPS Pharmaceutical Science and Technology, Vol. 7, No. 3, 2006.