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Isolation and Characterization of S-Enantiomer in Montelukast
Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
Jawaharlal Nehru Technological University Hyderabad, India
Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
- 1 Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
- 2 Jawaharlal Nehru Technological University Hyderabad, India
- 3 Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
- 4 Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
- 5 Inogent Laboratories Pvt Ltd., IDA-Nacharam, Hyderabad, India
American Journal of Analytical Chemistry·Volume 04 (2013)·Pages 56–61·Published 9 January 2013·DOI10.4236/ajac.2013.41008
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Abstract
A simple and rapid Supercritical Fluid Chromatography (SFC) Method has been developed to isolate and characterize the S-Isomer of Montelukast by using normal phase Amylose based AS-H with 250 mm × 30 mm , 5 microns column using a mobile phase system containing super critical fluid carbon dioxide (CO 2 ) and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230 nm. The isolated S-Isomer was characterized by using FT-IR, ESI-MS, HPLC and 1H NMR. The purity of isolated S-Isomer is about 98%.
Keywords(S)-IsomerMontelukastSupercritical Fluid Chromatography (SFC)
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