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Development of a Performant Method for Glucocapparin Determination in Boscia senegalensis Lam Ex. Poir.: A Study of the Variability
Institute of Food Technology (ITA), Hann-Dakar, Senegal
Regional Centre for Research in Ecotoxicology and Environmental Safety (CERES/Locustox), Dakar, Senegal
Institute of Food Technology (ITA), Hann-Dakar, Senegal
University of Liege, Gembloux Agro-Bio Tech, Unit of General and Organic Chemistry, Gembloux, Belgique
Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
University of Liege, Gembloux Agro-Bio Tech, Unit of General and Organic Chemistry, Gembloux, Belgique
Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
- 1 Institute of Food Technology (ITA), Hann-Dakar, Senegal
- 2 Regional Centre for Research in Ecotoxicology and Environmental Safety (CERES/Locustox), Dakar, Senegal
- 3 Institute of Food Technology (ITA), Hann-Dakar, Senegal
- 4 University of Liege, Gembloux Agro-Bio Tech, Unit of General and Organic Chemistry, Gembloux, Belgique
- 5 Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
- 6 Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
- 7 University of Liege, Gembloux Agro-Bio Tech, Unit of General and Organic Chemistry, Gembloux, Belgique
- 8 Gembloux Agro-Bio Tech, Unit Analysis, Quality, Risks—Laboratory of Analytical Chemistry, University of Liege, Gembloux, Belgium
American Journal of Analytical Chemistry·Volume 04 (2013)·Pages 104–110·Published 17 February 2013·DOI10.4236/ajac.2013.42014
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Abstract
This study describes a glucocapparin determination method. Based on rapeseed determination of glucosinolate (GSL), the equation of the average straight regression line is Y = 100.42X – 0.03 (R 2 = 0.9998). Enzymatic hydrolysis of glu cocapparin extracted from leaves and fruits of B. senegalensis , analyzed by SPME-GC-MS confirmed the presence of methylisothiocyanate as the main hydrolysis glucocapparin product. Monitoring glucocapparin contents in B. sene galensis leaves and fruits collected in 4 localities in Senegal showed differences between organs according localities and periods of harvest. Glucocapparin content was very high in dry season particularly in January and the lowest rates were recorded during the rainy period between August and November.
KeywordsBoscia SenegalensisValidation MethodGlucocapparinMonitoringSenegal
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