Using of <i>In-Situ</i> Mercury Film Sensor Hyphenated with Affinity Voltammetry for High Throughput Drug-Protein Binding Studies
- 1 Chemistry Department, Faculty of Science, Assiut University, Assiut, Egypt
- 2 Chemistry Department, Faculty of Science, Assiut University, Assiut, Egypt
Abstract
A new simple and reliable in-situ mercury film sensor coupled with affinity differential pulse stripping voltammetry (ADSPV) or affinity cyclic voltammetry (ACV) was investigated. The interaction of fenoprofen with bovine serum al bumin (BSA) onto the proposed electrochemical sensor was studied. The nature of the electrochemical process of feno profen by cyclic voltammetry was depicted. Reproducibility of the proposed method was checked giving a precision of 0.073 standard deviation. The limit of detection and limit of quantification were 7.0 and 22.0 nmol/L, respectively. Fenoprofen was interacted with BSA by 1:1 stoichiometry to form electroinactive supramolecular complex. The binding constant was precisely estimated by non-linear regression analysis based on the shifting of analyte peak potentials. The proposed experiments and data analysis could be use d to investigate the drug-protein binding constant within a short analysis time compared to other chromatographic techniques.
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