Determination of 17<i>α</i>-Methyltestosterone in Freshwater Samples of Tilapia Farming by High Performance Liquid Chromatography
- 1 Centre for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal
- 2 Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal
- 3 Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal
- 4 Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal
- 5 Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal
- 6 Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal
Abstract
17 α -methyltestosterone is used to induce the sex reversal of Tilapia sp. to obtain cultures mono - sex to an economically viable. This practice may lead to environmental contamination and problems in human health . T herefore methods need to be de velop ed to detect residues of 17 α -methyltestosterone in aqueous matrices. A simple high-performance liquid chro matographic method using ultraviolet detection (245 nm) and testosterone as internal standard has been developed for the monitoring 17 α -methyltestosterone in freshwater samples of tilapia aquaculture. The method described involves limited sample preparation as it includes a filtration followed by a single solid-phase extraction step using C18 cartridge. Validation data indicated that the HPLC-UV method for 17 α -methyltestosterone determination in the concen tration range of 50 - 2000 μg/L provided good linearity, sensitivity, accuracy and precision. Method performance was ef ficiently applied to monitoring the freshwater samples of fish ponds and the surrounding aquatic channels .
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