Analysis of <i>N</i>-Nitrosamine Migration from Rubber Teats and Soothers
- 1 Division of Food Additives, National Institute of Health Sciences, Tokyo, Japan
- 2 Division of Food Additives, National Institute of Health Sciences, Tokyo, Japan
- 3 Division of Food Additives, National Institute of Health Sciences, Tokyo, Japan
Abstract
A testing method for N -nitrosamines and N- nitrosatable substances in rubber teats and soothers was modified. N -Ni trosamines are generally analyzed using either a nitrogen chemiluminescence detector (NCD) or a thermal energy ana lyzer (TEA). However, because few testing laboratories are equipped with these devices, it is difficult to conduct these tests. Therefore, an analysis method for N -nitrosamines using the more widespread gas chromatography-mass spectro metry (GC-MS) method was improved. In addition, EN 12868 was used to prepare the test solutions because of its worldwide use and compliance with EU regulations. Using GC-MS, EN 12868 method targeting ten kinds of N -ni trosamines was modified. The determination limits of the method were 1.0 - 1.5 μg /kg for N -nitrosamines and 4 - 6 μg /kg for N- nitrosatable substances. Quantification was possible at 1/5 or less and 1/15 or less, respectively, of the re gulation values listed in EU Directive 93/11/EEC. In terms of application, there were no problems with the selectivity of the detector. The recoveries were 58% - 109% for N -nitrosamines and 59% - 102% for N- nitrosatable substances. Screening and verification were possible by measuring the amount of secondary amines in the boiled solution and mi gration solution.
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