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Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of <i>Leishmania</i> Genus Parasites

¹ Centro de Microscopia Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
² Centro de Microscopia Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
³ Centro de Microscopia Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
⁴ Departamento de Biología, Universidad Simón Bolívar, Caracas, Venezuela
⁵ Centro de Microscopia Electrónica, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
⁶ Escuela de Biología, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
⁷ Laboratorio de Espectroscopia Laser, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
⁸ Laboratorio de Espectroscopia Laser, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
⁹ Laboratorio de Espectroscopia Laser, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela
¹⁰ Laboratorio de Espectroscopia Laser, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela

American Journal of Analytical Chemistry·Volume 04 (2013)·Pages 100–108·Published 17 July 2013·DOI10.4236/ajac.2013.47A014

Abstract

In this work we present the preparation and functionalization of gold nanoparticles (AuNPs) for the detection of pres ence of gp63 glycoprotein in the surface of Leishmania genus parasites. AuNPs were prepared by induced laser ablation in a clean and biologically suitable media. The nanoparticles were functionalized with anti-gp63 lgG antibody in order to study the interaction with the glycoprotein component gp63 (63 kDa) present on the membrane surface of Leishma nia genus parasites. The functionalized AuNPs showed potential as a spectrometric indicator of the parasite existence, both by the detection of the presence of the gp 63 in solution and through the specific interaction with the parasites in vitro . The specificity of the study opens a new line of research on the use of modified nanoparticles in the development of a fast and easy assay for Leishmaniosis diagnostics.

Keywords&lti&gtLeishmania&lt/i&gtNanoparticlesSurface Plasmon Resonancegp63 Protein

A. Gultekin, A. Ersoz, D. Hur, N. Sariozlu, A. Denizli and R. Say, “Gold Nanoparticles Having Dipicolinic Acid Imprinted Nanoshell for Bacillus Cereus Spores Recognition,” Applied Surface Science, Vol. 256, No. 1, 2009, pp. 142-148. doi:10.1016/j.apsusc.2009.07.097
C. Wang, J. Wang, D. Liu and Z. Wang, “Gold Nanoparticle-Based Colorimetric Sensor for Studying the Interactions of β-Amyloid Peptide with Metallic Ions,” Talanta, Vol. 80, No. 5, 2010, pp. 1626-1631. doi:10.1016/j.talanta.2009.09.052
J. Wang, T. Duan, L. Sun, D. Liu and Z. Wang, “Functional Gold Nanoparticles for Studying the Interaction of Lectin with Glycosyl Complex on Living Cellular Surfaces,” Analytical Biochemistry, Vol. 392, No. 1, 2009, pp. 77-82. doi:10.1016/j.ab.2009.05.036
J. Kneipp, H. Kneipp, B. Wittig and K. Kneipp, “Novel Optical Nanosensors for Probing and Imaging Live Cells,” Nanomedicine, Vol. 6, No. 2, 2010, pp. 214-226. doi:10.1016/j.nano.2009.07.009
M. Velasco-Garcia, “Optical Biosensors for Probing at the Cellular Level: A Review of Recent Progress and Future Prospects,” Seminars in Cell & Developmental Biology, Vol. 20, No. 1, 2009, pp. 27-33. doi:10.1016/j.semcdb.2009.01.013
T. Vo-Dinh, “Nanosensing at the Single Cell Level,” Spectrochimica Acta Part B: Atomic Spectroscopy, Vol. 63, No. 2, 2008, pp. 95-103. doi:10.1016/j.sab.2007.11.027
H. Patra, S. Banerjee, U. Chaudhuri, P. Lahiri and A. Dasgupta, “Cell Selective Response to Gold Nanoparticles,” Nanomedicine, Vol. 3, No. 2, 2007, pp. 111-119. doi:10.1016/j.nano.2007.03.005
B. Leng, L. Zou, J. Jiang and H. Tian, “Colorimetric Detection of Mercuric Ion (Hg2+) in Aqueous Media Using Chemodosimeter-Functionalized Gold Nanoparticles,” Sensors and Actuators B: Chemical, Vol. 140, No. 1, 2009, pp. 162-169. doi:10.1016/j.snb.2009.03.074
I. Sendroiu, L. Gifford, A. Lupták and M. Corn, “Ultrasensitive DNA Microarray Biosensing via in Situ RNA Transcription-Based Amplification and Nanoparticle-Enhanced SPR Imaging,” Journal of the American Chemical Society, Vol. 133, No. 12, 2011, pp. 4271-4273. doi:10.1021/ja2005576
W. Hall, N. Ngatia and R. Van Duyne, “LSPR Biosensor Signal Enhancement Using Nanoparticle-Antibody Conjugates,” The Journal of Physical Chemistry C, Vol. 115, No. 5, 2011, pp. 1410-1414. doi:10.1021/jp106912p
P. Borst and M. Oullette, “New Mechanisms of Drug Resistance in Parasitic Protozoa,” Annual Review of Microbiology, Vol. 49, 1995, pp. 427-460. doi:10.1146/annurev.mi.49.100195.002235

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Study of Functionalized Gold Nanoparticles with Anti-gp63 IgG Antibody for the Detection of Glycoprotein gp63 in Membrane Surface of <i>Leishmania</i> Genus Parasites

Abstract

References (29)