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New Colorimetric Method for Lipases Activity Assay in Microbial Media
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt
Research and Development Center, Misr University for Science and Technology, Giza, Egypt
College of Biotechnology, Misr University for Science and Technology, Giza, Egypt
- 1 College of Biotechnology, Misr University for Science and Technology, Giza, Egypt
- 2 Research and Development Center, Misr University for Science and Technology, Giza, Egypt
- 3 College of Biotechnology, Misr University for Science and Technology, Giza, Egypt
American Journal of Analytical Chemistry·Volume 04 (2013)·Pages 442–444·Published 4 September 2013·DOI10.4236/ajac.2013.49055
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Abstract
A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol by Folin Ciocalteu reagent. Reaction mixture containing substrate 2.4 ml of phenyl acetate 165 μM in Tris HCl buffer, 0.1 M and pH 7, with 1% (v/v) Triton X-100) and 0.1 ml lipase is incubated at 40?C during 10 minutes and the absorbance was meas ured at 750 nm. Linearity was observed in the concentration range 0 - 0.8 g /L lipase.
KeywordsLipaseColorimetric MethodAssayPhenyl AcetateFolin
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