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Isolation and Characterization of R-Enantiomer in Ezetimibe
Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
- 1 Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
- 2 Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
- 3 Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
- 4 Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
- 5 Inogent laboratories Pvt Ltd., IDA-Nacharam, Jawaharlal Nehru Technological University Hyderabad, Hyderabad, India
American Journal of Analytical Chemistry·Volume 04 (2013)·Pages 488–495·Published 4 September 2013·DOI10.4236/ajac.2013.49062
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Abstract
A simple and rapid Supercritical Fluid Chromatography ( SFC) method has been developed to isolate and characterize R-Isomer of Ezetimi be by using normal phase Chiral Cel OD-H with 250 mm × 30 mm , 5 microns column using a mobile phase system containing super critical fluid carbondi oxide (Co2) and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230 nm. The isolated R-Isomer is characterized by using UV-vis, FT-IR, ESI-MS, HPLC 1 H and 13 C NMR. The purity of isolated R-Isomer is about 98%.
KeywordsIsolationCharacterization(R)-IsomerEzetimibeSupercritical Fluid Hromatography (SFC)
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