Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode — Oak Academic Publishing
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Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode
Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran Institute of Biotechnology, Urmia University, Urmia, Iran
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Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran
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Department of Chemical Engineering, Urmia University of Technology, Urmia, Iran
1 Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran Institute of Biotechnology, Urmia University, Urmia, Iran
2 Department of Chemistry, Faculty of Science, Urmia University, Urmia, Iran
3 Department of Chemical Engineering, Urmia University of Technology, Urmia, Iran
We report the fabrication of disposable and flexible Screen - Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H 2 O 2 with linear dependence on H 2 O 2 concentration in the range of 10 －5 to 10 －3 M; the detection limit was 4.5 × 10 －6 M.
A. L. Sanford, S. W. Morton, K. L. Whitehouse, H. M. Oara, L. Z. Lugo-Morales, J. G. Roberts and L. A. Sombers, Voltammetric Detection of Hydrogen Peroxide at Carbon Fiber Microelectrodes,” Analytical Chemistry, Vol. 82, No. 12, 2010, pp. 5205-5210. http://dx.doi.org/10.1021/ac100536s
C. Creanga, S. Serban, R. W. Pittson and N. El Murr, “No Calibration” Type Sensor in Routine Amperometric Biosensing—An Example of a Disposable Hydrogen Peroxide Biosensor,” Biosensors—Emerging Materials and Applications, 2011, pp. 141-152.
A. H. A. Al Obaidi, “Role of Airway Lactoperoxidase in Scavenging of Hydrogen Peroxide Damage in Asthma,” Annals of Thoracic Medicine Vol. 2, No. 3, 2007, pp. 107-110. http://dx.doi.org/10.4103/1817-1737.33698
H. L. Cai, “Hydrogen Peroxide Regulation of Endothelial Function: Origins, Mechanisms and Consequences,” Cardiovascular Research, Vol. 68, No. 1, 2005, pp. 26-36. http://dx.doi.org/10.1016/j.cardiores.2005.06.021
N. S. Brown and R. Bicknell, “Hypoxia and Oxidative Stress in Breast Cancer Oxidative Stress: Its Effects on the Growth, Metastatic Potential and Response to Therapy of Breast Cancer,” Breast Cancer Research, Vol. 3, No. 5, 2001, pp. 323-327. http://dx.doi.org/10.1186/bcr315
B. J. Tabner, O. M. A. El-Agnaf, S. Turnbull, M. J. German, K. E. Paleologou, Y. Hayashi, L. J. Cooper, N. J. Fullwood and D. Allsop, “Hydrogen Peroxide Is Generated during the Very Early Stages of Aggregation of the Amyloid Peptides Implicated in Alzheimer Disease and Familial British Dementia,” Journal of Biological Chemistry, Vol. 280, No. 43, 2005, pp. 35789-35792. http://dx.doi.org/10.1074/jbc.C500238200
M. Giorgio, M. Trinei, E. Migliaccio and P. G. Pelicci, “Hydrogen Peroxide: A Metabolic By-Product or a Common Mediator of Ageing Signals,” Nature Reviews Molecular Cell Biology, Vol. 8, No. 9, 2007, pp. 722-728. http://dx.doi.org/10.1038/nrm2240
E. S. Forzani, G. A. Rivas and V. M. Solis, Amperometric Determination of Dopamine on an Enzymatically Modified Carbon Paste Electrode,” Journal of Electroanalytical chemistry, Vol. 382, No. 1-2, 1995, pp. 33-40. http://dx.doi.org/10.1016/0022-0728(94)03640-O
J. Kulys, L. Gorron, E. Domingues, J. Emneus and H. Jarskog, “Electrochemical Characterization of Carbon pastes Modified with Proteins and Polycations,” Journal of Electroanalytical Chemistry, Vol. 372, No. 1-2 , 1994, pp. 49-55. http://dx.doi.org/10.1016/0022-0728(93)03262-N
Z. Wang, J. Yi and S. Yang, “Direct Electrochemistry and Electrocatalysis of Hemoglobin Incorporated in Composite Film Based on Diblock Weak Polyelectrolyte PHA-EMA-b PDMAEMA and Multi-Walled Carbon Nanotubes,” Sensors and Actuators B: Chemical, Vol. 176, 2013, pp. 211-216. http://dx.doi.org/10.1016/j.snb.2012.10.002
M. C. Y. Chang, A. Pralle, E. Y. Isacoff and C. J. Chang, “A Selective, Cell-Permeable Optical Probe for Hydrogen Peroxide in Living Cells,” Journal of the American Chemical Society, Vol. 126, No. 47, 2004, pp. 15392-15393. http://dx.doi.org/10.1021/ja0441716
J. Li, P. K. Dasgupta and G. A. Tarver, “Pulsed Excitation Source Multiplexed Fluorometry for the Simultaneous Measurement of Multiple Analytes. Continuous Measurement of Atmospheric Hydrogen Peroxide and Methyl Hydroperoxide,” Analytical Chemistry, Vol. 75, No. 5, 2003, pp. 1203-1210. http://dx.doi.org/10.1021/ac026234d
M. R. Miah and T. Ohsaka, “Cathodic Detection of H2O2 using Iodide-Modified Gold Electrode in Alkaline Media,” Analytical Chemistry, Vol. 78, No. 4, 2006, pp. 1200-1205. http://dx.doi.org/10.1021/ac0515935
M. Magro, D. Baratella, N. Pianca, A. Toninello, S. Grancara, R. Zboril and F. Vianello, Electrochemical Determination of Hydrogen Peroxide Production by Isolated Mitochondria: A Novel Nanocomposite Carbon-Maghemite Nanoparticle Electrode,” Sensors and Actuators B: Chemical, Vol. 176, 2013, pp. 315-322. http://dx.doi.org/10.1016/j.snb.2012.09.044
E. Zapp, V. Nascimento, D. Dambrowski, A. L. Braga and I. C. Vieira, “Bio-Inspired Sensor Based on Glutathione Peroxidase Mimetic for Hydrogen Peroxide Detection,” Sensors and Actuators B: Chemical, Vol. 176, 2013, pp. 782-788. http://dx.doi.org/10.1016/j.snb.2012.09.072
K. Thenmozhi and S. S. Narayanan, “Surface Renewable Sol-Gel Composite Electrode Derived from 3-Aminopropyl Trimethoxy Silane with Covalently Immobilized Thionin,” Biosensors and Bioelectronics, Vol. 23, No. 5, 2007, pp. 606-612. http://dx.doi.org/10.1016/j.bios.2007.06.003
Y. Xiao, H. X. Ju and H. Y. Chen, “Hydrogen Peroxide sensor Based on Horseradish Peroxidase-Labeled Au Colloids Immobilized on Gold Electrode Surface by Cysteamine Monolayer,” Analytica Chimica Acta, Vol. 391, No. 1, 1999, pp. 73-82. http://dx.doi.org/10.1016/S0003-2670(99)00196-8
A. K. Upadhyay, T. W. Ting and S. M. Chen, “Amperometric Biosensor for Hydrogen Peroxide Based on Coimmobilized Horseradish Peroxidase and Methylene Green in Ormosils Matrix with Multiwalled Carbon Nanotubes,” Talanta, Vol. 79, No. 1, 2009, pp. 38-45. http://dx.doi.org/10.1016/j.talanta.2009.03.010
S. Li, X. Zhu, W. Zhang, G. Xie and W. Feng, “Hydrogen Peroxide Biosensor Based on Gold Nanoparticles/Thionine/Gold Nanoparticles/Multi-Walled Carbon Nanotubes-Chitosans Composite Film-Modified Electrode,” Applied Surface Science, Vol. 258, No. 7, 2012, pp. 2802-2807. http://dx.doi.org/10.1016/j.apsusc.2011.10.138
I. Palchetti and M. Mascini, “Electrochemical Adsorption Technique for Immobilization of Single-Stranded Oligonucleotides onto Carbon Screen-Printed Electrodes,” Topics in Current Chemistry, Vol. 261, 2006, pp. 27-43. http://dx.doi.org/10.1007/b135774
T. Schüler, T. Asmus, W. Fritzsche and R. Moller, “Screen Printing as Cost-Efficient Fabrication Method for DNA-Chips with Electrical Readout for Detection of Viral DNA,” Biosensors and Bioelectronics, Vol. 24, No. 7, 2009, pp. 2077-2084. http://dx.doi.org/10.1016/j.bios.2008.10.028
R. Pilloton, et al., “Screen Printing for Chemical Sensor and Biosensor Production,” II Workshop on Chemical Sensors and Biosensors, Rome, Italy
J. P. Hart and S. A. Wring, “Recent Developments in the Design and Application of Screen-Printed Electrochemical Sensors for Biomedical, Environmental and Industrial Analyses,” Trends in Analytical Chemistry, Vol. 16, No. 2, 1997, pp. 89-103. http://dx.doi.org/10.1016/S0165-9936(96)00097-0
M. A. Sirvent, A. Merkoci and S. Alegret, “Configurations Used in the Design of Screen-Printed Enzymatic Biosensors. A Review, Sensors and Actuators B: Chemical, Vol. 69, No. 1-2, 2000, pp. 153-163. http://dx.doi.org/10.1016/S0925-4005(00)00536-0
T. M. O Regan, M. Pravda, C. K. O Sullivan and G. G. Guibault, “Development of a Disposable Immunosensor for the Detection of Human Heart Fatty-Acid Binding Protein in Human Whole Blood Using Screen-Printed Carbon Electrodes,” Talanta, Vol. 57, No. 3, 2002, pp. 501-510. http://dx.doi.org/10.1016/S0039-9140(02)00047-4
J. J. Rippeth, T. D. Gibson, J. P. Hart, I. C. Harthey and G. Nelson, “Flow-Injection Detector Incorporating a Screen-Printed Disposable Amperometric Biosensor for Monitoring Organophosphate pesticides,” Analyst, Vol. 122, No. 11, 1997, pp. 1425-1430. http://dx.doi.org/10.1039/a704291d
R. Kataky, R. Dell and P. K. Senanayake, “CyclodextrinModified Biosensors: Comparision of Cyclodextrin-Linked Forrocenes as Mediators in Sol-Gel and Screen-Printed Formats for Sensing Acetylcholine,” Analyst, Vol. 126, No. 11, 2001, pp. 2015-2019. http://dx.doi.org/10.1039/b105465c
A. Avramescu, T. Noguer, M. Avramescu and J. L. Marty, “Screen-Printed Biosensors for the Control of Wine Quality Based on Lactate and Acetaldehyde Determination,” Analytica Chimica Acta, Vol. 458, No. 1, 2002, pp. 203-213. http://dx.doi.org/10.1016/S0003-2670(01)01580-X
J. Wang, Q. Chen, M. Pedrero and J. M. Pingarron, “Screen-Printed Amperometric Biosensors for Glucose and Alcohols Based on Ruthenium-Dispersed Carbon Inks,” Analytica Chimica Acta, Vol. 300, No. 1-3, 1995, pp. 111-116. http://dx.doi.org/10.1016/0003-2670(94)00357-R
R. Gupta and N. K. Chaudhury, “Entrapment of Biomolecules in Sol-Gel Matrix for Applications in Biosensors: Problems and Future Prospects,” Biosensors and Bioelectronics, Vol. 22, No. 11, 2007, pp. 2387-2399. http://dx.doi.org/10.1016/j.bios.2006.12.025
T. M. Park, E. I. Iwuoha, M. R. Smyth, R. Freaney and A. J. McShane, “Sol-gel Based Amperometric Biosensor Incorporating an Osmium Redox Polymer as Mediator for Detection of L-Lactate,” Talanta, Vol. 44, No. 6, 1997, pp. 973-978. http://dx.doi.org/10.1016/S0039-9140(96)02164-9
U. Narang, P. N. Prasad, F. V. Bright, K. Ramanathan, N. D. Kumar, B. D. Malhotra, M. N. Kamalasanan and S. Chandra, “Glucose Biosensor Based on a Sol-Gel Derived Platform,” Analytical Chemistry, Vol. 66, No. 19, 1994, pp. 3139-3144. http://dx.doi.org/10.1021/ac00091a023
L. L. Hench and J. K. West, “The Sol-Gel Process,” Chemical Reviews, Vol. 90, No. 1, 1990, pp. 33-72. http://dx.doi.org/10.1021/cr00099a003
D. Avnir, “Organic Chemistry within Ceramic Matrices: Doped Sol-Gel Materials,” Accounts of Chemical Research, Vol. 28, No. 8, 1995, pp. 328-334. http://dx.doi.org/10.1021/ar00056a002
E. Llobet, P. Ivanov, X. Vilanova, J. Brezmes, J. Hubalek, K. Malysz, I. Gràcia, C. Cané and X. Correig, “ScreenPrinted Nanoparticle Tin Oxide Films for High-Yield Sensor Microsystems,” Sensors and Actuators B: Chemical, Vol. 96, No. 1-2, 2003, pp. 94-104. http://dx.doi.org/10.1016/S0925-4005(03)00491-X
Y. Li, W. Bu, L. Wu and C. Sun, “A New Amperometric Sensor for the Determination of Bromate, Iodate and Hydrogen Peroxide Based on Titania Sol-Gel Matrix for Immobilization of Cobalt Substituted Keggin-Type Cobalttungstate Anion by Vapor Deposition Method,” Sensors and Actuators B: Chemical, Vol. 107, No. 2, 2005, pp. 921-928. http://dx.doi.org/10.1016/j.snb.2004.12.040
J. H. Yu and H. X. Ju, “Amperometric Biosensor for Hydrogen Peroxide Based on Hemoglobin Entrapped in Titania Sol-Gel Film,” Analytica Chimica Acta, Vol. 486, No. 2, 2003, pp. 209-216. http://dx.doi.org/10.1016/S0003-2670(03)00508-7
J. H. Yu, S. Q. Liu and H. X. Ju, “Glucose Sensor for Flow Injection Analysis of Serum Glucose Based on Immobilization of Glucose Oxidase in Titania Sol-Gel Membrane,” Biosensors and Bioelectronics, Vol. 19, No. 4, 2003, pp. 401-409. http://dx.doi.org/10.1016/S0956-5663(03)00199-4
Z. T. Jiang and Y. M. Zuo, “Synthesis of Porous Titania Microspheres for HPLC Packings by Polymerization-Induced Colloid Aggregation (PICA),” Analytical Chemistr, Vol. 73, No. 3, 2001, pp. 686-688. http://dx.doi.org/10.1021/ac001008u
T. Lopez, J. H. Ventura, R. Gomez, F. Tzompantzi, E. Sanchez, X. Bokhimi and A. Garcia, Photodecomposition of 2,4-Dinitroaniline on Li/TiO2 and Rb/TiO2 Nanocrystallite Sol-Gel Derived Catalysts,” Journal of Molecular Catalysis A, Vol. 167, No. 1-2, 2001, pp. 101-107. http://dx.doi.org/10.1016/S1381-1169(00)00496-9
E. Milella, F. Cosentino, A. Licciulli and C. Massaro, “Preparation and Characterization of Titania/Hydroxyapatite Composite Coatings Obtained by Sol-Gel Process,” Biomaterials, Vol. 22, No. 11, 2001, pp. 1425-1431. http://dx.doi.org/10.1016/S0142-9612(00)00300-8
J. Yu and H. Ju, Pure Organic Phase Phenol Biosensor Based on Tyrosinase Entrapped in a Vapor Deposited Titania Sol-Gel Membrane,” Electroanalysis, Vol. 16, No. 16, 2004, pp. 1305-1310. http://dx.doi.org/10.1002/elan.200302951
P. Kouvaris, A. Delimitis, V. Zaspalis, D. Papadopoulos, S. A. Tsipas and N. Michailidis, “Green Synthesis and Characterization of Silver Nanoparticles Produced Using Arbutus Unedo Leaf Extract,” Materials Letters, Vol. 76, 2012, pp. 18-20. http://dx.doi.org/10.1016/j.matlet.2012.02.025
L. Zhang, Y. Li, L. Zhang, D. W. Li, D. Karpuzov and Y. T. Long, Electrocatalytic Oxidation of NADH on Graphene Oxide and Reduced Graphene Oxide Modified Screen-Printed Electrode,” International Journal of Electrochemical Science, Vol. 6, No. 1, 2011, pp. 819-829.
R. G. González, M. T. F. Abedul, A. Pernía and A. C. García, “Electrochemical Characterization of Different Screen-Printed Gold Electrodes, Electrochimica Acta, Vol. 53, No. 8, 2008, pp. 3242-3249. http://dx.doi.org/10.1016/j.electacta.2007.07.059
M. Mathew and N. Sandhyarani, “A Novel Electrochemical Sensor Surface for the Detection of Hydrogen Peroxide Using Cyclic Bisureas/Gold Nanoparticle Composite,” Biosensors and Bioelectronics, Vol. 28, No. 1, 2011, pp. 210-215. http://dx.doi.org/10.1016/j.bios.2011.07.020
A. Ahmadalinezhad, G. Wu and A. Chen, “Mediator-Free Electrochemical Biosensor Based on Buckypaper with Enhanced Stability and Sensitivity for Glucose Detection,” Biosensors and Bioelectronics, Vol. 30, No. 1, 2011, pp. 287-293. http://dx.doi.org/10.1016/j.bios.2011.09.030
K. Zhou, Y. Zhu, X. Yang, J. Luo, C. Li and S. Luan, “A Novel Hydrogen Peroxide Biosensor Based on Au-Graphene-HRP-Chitosan Biocomposites,” Electrochimica Acta, Vol. 55, No. 9, 2010, pp. 3055-3060. http://dx.doi.org/10.1016/j.electacta.2010.01.035
A. K. Abelskov, A. T. Smith, C. B. Rasmussen, H. B. Dunford and K. G. Welinder, “pH-Dependence and Structural Interpretation of the Reactions of Coprinus Cinereus Peroxidase with Hydrogen Peroxide, Ferulic Acid, and 2,2’-Azinobis(3-ethyl benzthiazoline-6-sulfonic Acid),” Biochemistry, Vol. 36, No. 31, 1997, pp. 9453-9463. http://dx.doi.org/10.1021/bi970387r
S. K. Arya, A. K. Prusty, S. P. Singh, P. R. Solanki, M. K. Pandey, M. Datta and B. D. Malhotra, “Cholesterol Biosensor Based on N-(2-Aminoethyl)-3-aminopropyltrimethoxysilane Self-Assembled Monolayer,” Analytical Biochemistry, Vol. 363, No. 2, 2007, pp. 210-218. http://dx.doi.org/10.1016/j.ab.2007.01.029
F. Li, Y. Feng, Z. Wang, L. Yang, L. Zhuo and B. Tang, “Direct Electrochemistry of Horseradish Peroxidase Immobilized on the Layered Calcium Carbonate-Gold Nanoparticles Inorganic Hybrid Composite,” Biosensors and Bioelectronics, Vol. 25, No. 10, 2010, pp. 2244-2248. http://dx.doi.org/10.1016/j.bios.2010.03.006
K. Zhou, Y. Zhu, X. Yang, C. Li, “Preparation and Application of Mediator-Free H2O2 Biosensors of Graphene-Fe3O4 Composites,” Electroanalysis, Vol. 23, No. 4, 2011, pp. 862-869. http://dx.doi.org/10.1002/elan.201000629
G. Mengmeng, Y. Yunhui, W. Zhijie, S. Guoli and Y. Ruqin, “A Mediator-free Horseradish Peroxidase Biosensor Based on Concanavalin A,” Chinese Journal of Analytical Chemistry, Vol. 34, No. 3, 2006, pp. 399-402. http://dx.doi.org/10.1016/S1872-2040(06)60021-2