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A New Method for the Determination of Cyanide Ions and Their Quantification in Some Senegalese Cassava Varieties
Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
Département Génie Chimique et Biologie Appliquée, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal
Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
Unité Analyses, Qualité, Risques et Laboratoire de Chimie Analytique, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgique
Unité Analyses, Qualité, Risques et Laboratoire de Chimie Analytique, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgique
- 1 Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
- 2 Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
- 3 Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
- 4 Département Génie Chimique et Biologie Appliquée, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal
- 5 Institut de Technologie Alimentaire, Route des Pères Maristes, Dakar, Sénégal
- 6 Unité Analyses, Qualité, Risques et Laboratoire de Chimie Analytique, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgique
- 7 Unité Analyses, Qualité, Risques et Laboratoire de Chimie Analytique, Gembloux Agro-Bio Tech, Université de Liège, Gembloux, Belgique
American Journal of Analytical Chemistry·Volume 05 (2014)·Pages 181–187·Published 17 February 2014·DOI10.4236/ajac.2014.53022
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Abstract
Cassava ( Manihot esculenta Crantz ) is a starchy staple food that previous researches have showed to contain cyanogenic compounds, precursors of hydrocyanic acid, undoubtedly toxic for humans. With the aim to determine food security in cassava, this study developed a simple, fast and less expensive step for quantifying cyanide ions by using micro-diffusion with modified Conway cells. After an enzymatic degradation, the cyanide ions were quantified by electrochemical procedures. The validation of this method is estimated. The concentration of cyanide ions at different part of the samples was determined. The results showed high toxicity in some fresh Senegalese consumed cassava varieties (>100 mg HCN·kg ﹣1 ). However, in the processed cassava products, less than 10 mg HCN·kg ﹣1 was found in the different varieties studied except for the chips where the levels of CN ﹣ contents were important (>49 mg HCN·kg ﹣1 ).
KeywordsCassavaCyanogenic GlycosidesCyanide IonMicro-DiffusionToxicity
- J. H. Cock, “Cassava: New Potential for a Neglected Crop,” Westview Press, Boulder, 1985, p. 191.
- FAOSTAT, 2013. http://faostat.fao.org/site/339/default.aspx
- C. A. Idible, “Isolation of Pure Cassava Linamarin as an Anti Cancer Agent,” Master of Science in Engineering, University of the Witwatersrand, Faculty of Engineering and the Built Environment, Johannesburg, 2006, 109 p.
- E. A. Ouegnin, “Contribution à L’étude de L’acide Cyanhydrique dans un Produit de Transformation du Manioc: L’Attiéké,” Thèse de Doctorat en Pharmacie, Université Cheikh Anta Diop de Dakar, Sénégal, No. 22, 1988.
- S. C. Kobawila, D. Louembe, S. Keleke, J. Hounhouigan and C. Gamba, “Reduction of the Cyanide Content during Fermentation of Cassava Roots and Leaves to Produce Bikedi and Ntobambodi, Two Food Products from Congo,” African Journal of Biotechnology, Vol. 4, No. 7, 2005, pp. 689-696. http://www.ajol.info/index.php/ajb/article/view/15168/59486
- C. Pohlandt, E. A. Jones and A. F. Lee, “A Critical Evaluation of Methods Applicable to the Determination of Cyanides,” Journal of the South African Institute of Mining and Metallurgy, Vol. 83, 1983, pp. 11-19.
- M. Ernesto, A. P. Cardoso, D. Nicala, E. Mirione, F. Massaza, J. Cliff, M. R. Haque and J. H. Bradbury, “Persistent Konzo and Cyanide Toxicity from Cassava in Northern Mozambique,” Acta Tropica, Vol. 82, No. 3, 2002, pp. 357-362. http://dx.doi.org/10.1016/S0001-706X(02)00042-6
- Y. Diallo, M. T. Gueye, M. Sakho, P. B. Darboux, A. Kane, J.-P. Barthelemy and G. Lognay, “Importance Nutritionnelle du Manioc et Perspectives pour L’alimentation de base au Sénégal-Synthèse Bibliographique,” Biotechnology, Agronomy, Society and Environment, Vol. 17, No. 4, 2013, pp. 634-643.
- ANSD (Agence Nationale de la Statistique et de la Démographie), “Bulletin Mensuel des Statistiques Economiques,” Dakar: DAPS/MA, 2011, p. 8. http://www.ansd.sn/publications/conjoncturelles/...statistique/BullStat_10_11.pdf
- A. A. Adeniji, L. A. Ega, M. O. Akoroda, B. O. Ugwu and A. Balogum, “Global Strategy for Cassava Development. Nigeria: A Country Case Study,” Department of Agriculture, Federal Ministry of Agriculture and Natural Resources, Available Developments of New Techniques for Pari Production, 2001. http://www.globalcassavastrategy.net
- S. Williams, “Hydrocyanic Acid in Beans, Alkaline Titration Method,” Official Methods of Analysis of the Association of Official Analytical Chemist, AOAC, Inc., Arlington, 1990, p. 1213.