Flow Injection Amperometric Determination of Phenol and Chlorophenols at Single Wall Carbon Nanotube Modified Glassy Carbon Electrode — Oak Academic Publishing
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Flow Injection Amperometric Determination of Phenol and Chlorophenols at Single Wall Carbon Nanotube Modified Glassy Carbon Electrode
Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
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Department of Chemistry and Chemical Technology, National University of Lesotho, Roma, Lesotho
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Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
1 Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
2 Department of Chemistry and Chemical Technology, National University of Lesotho, Roma, Lesotho
3 Department of Chemistry, Addis Ababa University, Addis Ababa, Ethiopia
Single wall carbon nanotube modified glassy carbon electrode (SWCNT/GCE) was used for flow-injection analy sis (FIA) for phenolic compounds (phenol (P), 4-chlorophenol (CP), 2,4-dichlorophenol (DCP), 2,4,6-trichlor phenol (TCP) and pentachlorophenol (PCP)). Experimental variables such as the detection potential, flow rate and pH of the carrier solution, 0.1 M sodium acetate, were optimized. Under these conditions , the designed electrode showed a very good performance for the amperometric measurements, with no need to apply a cleaning or pre-treatment procedure. The operational stability was tested with 20 repetitive injections of each analyte and was found to be good. The analytical performance of the SWCNT/GCE electrode under flow through conditions was tested and was found to be impressive. When it is compared with other enzymatic and non-enzymatic sen sors , it shows wider dynamic range for the detection of phenolic compounds with low limits of detection. These results suggest that the method is quite useful for monitoring and analyzing phenols and chlorophenols.
KeywordsSingle Wall Carbon Nanotube Modified ElectrodePhenol and ChlorophenolsAmperometryFlow Injection Analysis
I. E. Mülazimoglu, A. D. Mülazimoglu and E. Yilmaz, “Determination of Quantitative Phenol in Tap Water Samples as Electrochemical Using 3,3’-Diaminobenzidine Modified Glassy Carbon Sensor Electrode,” Desalination, Vol. 268, No. 1, 2011, pp. 227-232. http://dx.doi.org/10.1016/j.desal.2010.10.033
C. B. Jacobs, M. J. Peairs and B. J. Venton, “Review: Carbon Nanotube Based Electrochemical Sensors for Biomolecules,” Analytica Chimica Acta, Vol. 662, No. 2, 2010, pp. 105-127. http://dx.doi.org/10.1016/j.aca.2010.01.009
K. Balasubramanian and M. Burghard, “Biosensors Based on Carbon Nanotubes,” Analytical and Bioanalytical Chemistry, Vol. 385, No. 3, 2006, pp. 452-468. http://dx.doi.org/10.1007/s00216-006-0314-8
S. K. Vashist, D. Zheng, K. Al-Rubeaan, J. H. T. Luong and F.-S. Sheu, “Advances in Carbon Nanotube Based Electrochemical Sensors for Bioanalytical Applications,” Biotechnology Advancement, Vol. 29, No. 2, 2011, pp. 169-188. http://dx.doi.org/10.1016/j.biotechadv.2010.10.002
D. W. H. Fam, A. I. Palaniappan, A. I. Y. Tok, B. Liedberg and S. M. Moochhala, “A Review on Technological Aspects Influencing Commercialization of Carbon Nanotube Sensors,” Sensors and Actuators B, Vol. 157, No. 1, 2011, pp. 1-7. http://dx.doi.org/10.1016/j.snb.2011.03.04
K. Scida, P. W. Stege, G. Haby, G. A. Messina and C. D. García, “Recent Applications of Carbon-Based Nanomaterials in Analytical Chemistry: Critical Review,” Analytica Chimica Acta, Vol. 69, No. 1, 2011, pp. 6-17. http://dx.doi.org/10.1016/j.aca.2011.02.025
P. Yanez-Sedeno, J. Riu, J. M. Pingarron and F. X. Rius, “Electrochemical Sensing Based on Carbon Nanotubes,” TrAc-Trends in Analytical Chemistry, Vol. 29, No. 9, 2010, pp. 939-953. http://dx.doi.org/10.1016/j.trac..2010.06.00
A. J. Ahammad, J. Lee and M. A. Rahman, “Electrochemical Sensors Based on Carbon Nanotubes,” Sensors, Vol. 9, No. 1, 2009, pp. 2289-2319. http://dx.doi.org/10.3390/s90402289
J. Wang, R. P. Deo and M. Musameh, “Stable and Sensitive Electrochemical Detection of Phenolic Compounds at Carbon Nanotube Modified Glassy Carbon Electrodes,” Electroanalysis, Vol. 15, No. 23-24, 2003, pp. 1830-1834. http://dx.doi.org/10.1002/elan.200302772
Q. Zhao, L. Guan, Z. Gu and Q. Zhuang, “Determination of Phenolic Compounds Based on the Tyrosinase-Single Walled Carbon Nanotubes Sensor,” Electroanalysis, Vol. 17, No. 1, 2005, pp. 85-88. http://dx.doi.org/10.10.10.1002/elan.200403123
A. S. Arribas, E. Bermejo, M. Chicharro, A. Zapardiel, G. L. Luque, N. F. Ferreyra and G. A. Rivas, “Analytical Applications of Glassy Carbon Electrodes Modified with Multi-Wall Carbon Nanotubes Dispersed in Polyethylenimine as Detectors in Flow Systems,” Analytica Chimica Acta, Vol. 596, No. 2, 2007, pp. 183-194. http://dx.doi.org/10.1016/j.aca.2007.06.014
S. Sundaram and S. K. Annamalai, “Selective Immobilization of Hydroquinone on Carbon Nanotube Modified Electrode via Phenol Electro-Oxidation Method and Its Hydrazine Electro-Catalysis and Escherichia Coli Anti-bacterial Activity,” Electrochimica Acta, Vol. 62, 2012, pp. 207-217. http://dx.doi.org/10.1016/j.electacta.2011.12.044
M. Naczk and F. Shahidi, “Phenolics in Cereals, Fruits and Vegetables: Occurrence, Extraction and Analysis,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 41, No. 5, 2006, pp. 1523-1542. http://dx.doi.org/10.1016/j.jpba.2006.04.002
A. Simic, D. Manojlovic, D. Segan and M. Todorovic, “Electrochemical Behavior and Antioxidant and Prooxidant Activity of Natural Phenolics,” Molecules, Vol. 12, No. 10, 2007, pp. 2327-2340. http://dx.doi.org/10.3390/12102327
C. Proestos, D. Sereli and M. Komaitis, “Determination of Phenolic Compounds in Aromatic Plants by RP-HPLC and GC-MS,” Food Chemistry, Vol. 95, No. 1, 2006, pp. 44-52. http://dx.doi.org/10.1016/j.foodchem.2004.12.016
A. S. Arribas, M. Martinez-Fernandez and M. Chicharro, “The Role of Electroanalytical Techniques in Analysis of Polyphenols in Wine,” TrAc Trends in Analytical Chemistry, Vol. 34, 2012, pp. 78-96. http://dx.doi.org/10.1016/j.trac.2011.10.015
A. Penalver, E. Pocurull, F. Borrull and R. M. Marce, “Solid-Phase Microextraction Coupled to High-Performance Liquid Chromatography to Determine Phenolic Compounds in Water Samples,” Journal of Chromatography A, Vol. 953, No. 1-2, 2002, pp. 79-87. http://dx.doi.org/10.1016/S0021-9673(02)00113-9
G. W. Muna, V. Quaiserova-Mocko and G. M. Swain, “The Analysis of Chlorinated Phenol Solutions by Capillary Electrophoresis Coupled with Direct and Indirect Amperometric Detection Using a Boron-Doped Diamond Microelectrode,” Electroanalysis, Vol. 17, No. 13, 2005, pp. 1160-1170. http://dx.doi.org/10.1002/elan.200403230
“Phenols in Federal Register: Environment Protection Agency Method 604,” Part VIII, 40 CFR Part 136, 1984, pp. 58-66.
“Phenols by Gas Chromatography, Capillary Column Technique: Environment Protection Agency Method 804,” Part 1, Washington DC, 1995, pp. l-28.
D. Puig and D. Barcelo, “Determination of Phenolic Compounds in Water and Waste Water,” TrAc Trends in Analytical Chemistry, Vol. 15, No. 8, 1996, pp. 362-365. http://dx.doi.org/10.1016/0165-9936(96)00057-X
J.-S. Park, M. T. Brown and T. Han, “Phenol Toxicity to the Aquatic Macrophyte Lemna Paucicostata,” Aquatic Toxicology, Vol. 106-107, 2012, pp. 182-188. http://dx.doi.org/10.1016/j.aquatox.2011.10.004
M. Krizman, D. Baricevic and M. Prosek, “Determination of Phenolic Compounds in Fennel by HPLC and HPLC-MS Using a Monolithic Reversed-Phase Column,” Journal of Pharmaceutical and Biomedical Analysis, Vol. 43, No. 2, 2007, pp. 481-485. http://dx.doi.org/10.1016/j.jpba.2006.07.029
M. Tasioula-Margari and O. Okogeri, “Simultaneous Determination of Phenolic Compounds and Tocopherols in Virgin Olive Oil Using HPLC and UV Detection,” Food Chemistry, Vol. 74, No. 3, 2001, pp. 377-383. http://dx.doi.org/10.1016/S0308-8146(01)00176-5
F. Zhou, X. Li and Z. Zeng, “Determination of Phenolic Compounds in Wastewater Samples Using a Novel Fiber by Solid-Phase Microextraction Coupled to Gas Chromatography,” Analytica Chimica Acta, Vol. 538, No. 1-2, 2005, pp. 63-70. http://dx.doi.org/10.1016/j.aca.2005.02.009
Y. Liang, W. Cao, W.-J. Chen, X.-H. Xiao and J.-B. Zheng, “Simultaneous Determination of Four Phenolic Components in Citrus Honey by High Performance Liquid Chromatography Using Electrochemical Detection,” Food Chemistry, Vol. 114, No. 4, 2009 pp. 1537-1541. http://dx.doi.org/10.1016/j.foodchem.2008.11.024
E. Hurtado-Fernández, M. Gómez-Romero, A. Carrasco-Pancorbo and A. Fernández-Gutiérrez, “Application and Potential of Capillary Electroseparation Methods to Determine Antioxidant Phenolic Compounds from Plant Food Material,” Journal of. Pharmaceutical and Biomedical Analysis, Vol. 53, No. 5, 2010, pp. 1130-1160. http://dx.doi.org/10.1016/j.jpba.2010.07.028
A. M. Naley, “Analysis of Phenols in Sea Water by Fluorometry: Direct Analysis of the Water Phase,” Bulletin of Environmental Contamination and Toxicology, Vol. 3, No. 4, 1983, pp. 494-500. http://dx.doi.org/10.1007/BF01622283
Y. Tsuruta, S. Watanabe and H. Inoue, “Fluorometric Determination of Phenol and p-Cresol in Urine by Precolumn High-Performance Liquid Chromatography Using 4-(N-Phthalimidinyl)benzenesulfonyl Chloride,” Analytical Biochemistry, Vol. 243, No. 1, 1996, pp. 86-91. http://dx.doi.org/10.1006/abio.1996.0485
M. Trojanowicz, “Recent Developments in Electrochemical Flow Advantages Detections—A Review Part II. Liquid Chromatography,” Analytica Chimica Acta, Vol. 688, No. 1, 2011, pp. 8-35. http://dx.doi.org/10.1016/j.aca.2010.12.024
B. Fuhrmann and U. Spohn, “An Enzymatic Amplification Flow Injection Analysis (FIA) System for the Sensitive Determination of Phenol,” Biosensor and Bioelectronics, Vol. 13, No. 7-8, 1998, pp. 895-902. http://dx.doi.org/10.1016/S0956-5663(98)00061-X
F. Maya, J. M. Estela and V. Cerdà, “Flow Analysis Techniques as Effective Tools for the Improved Environmental Analysis of Organic Compounds Expressed as Total Indices,” Talanta, Vol. 81, No. 1-2, 2010, pp. 1-8. http://dx.doi.org/10.1016/j.talanta.2010.01.028
E. J. Liorent-Martinez, P. Ortega-Barrales, M. L. Fernandez de Cordova and A. Ruiz-Mendina, “Trends in Flow-Based Analytical Methods Applied to Pesticide Detection: A Review,” Analytica Chimica Acta, Vol. 684, No. 1-2, 2011, pp. 30-39. http://dx.doi.org/10.1016/j.aca.2010.10.036
Y.-C. Tsai and C.-C. Chiu, “Amperometric Biosensors Based on Multiwalled Carbon Nanotube-Nafion-Tyrosinase Nanobiocomposites for the Determination of Phenolic Compounds,” Sensors and Actuators B, Vol. 125, No. 1, 2007, pp. 10-16. http://dx.doi.org/10.1016/j.snb.2007.01.032
B. Haghighi, L. Gorton, T. Ruzgas and L. J. Jönsson, “Characterization of Graphite Electrodes Modified with Laccase from Trametes versicolor and Their Use for Bio-electrochemical Monitoring of Phenolic Compounds in Flow Injection Analysis,” Analytica Chimica Acta, Vol. 487, No. 1, 2003, pp. 3-14. http://dx.doi.org/10.1016/S0003-2670(03)00077-1
R. S. Freire, N. Duran and L. Kubota, “Development of a Laccases-Based Flow Injection Electrochemical Biosensor for the Determination of Phenolic Compounds and Its Application for Monitoring Remediation of Kraft EI Paper Mill Effluent,” Analytica Chimica Acta, Vol. 463, No. 2, 2002, pp. 229-238. http://dx.doi.org/10.1016/S0003-2670(02)00417-8
S. Korkut, B. Keskinler and E. Erhan, “An Amperometric Biosensor Based on Multiwalled Carbon Nanotube-Poly(pyrrole)-horseradish Peroxidase Nanobiocomposite Film for Determination of Phenol Derivatives,” Talanta, Vol. 76, No. 5, 2008, pp. 1147-1152. http://dx.doi.org/10.1016/j.talanta.2008.05.016
S. K. Ozoner, F. Yilmaz, A. Celik, B. Keskinler and E. Erhan, “A Novel Poly(Glycine methacrylate-co-3-thieny-lmethyl methacrylate)-polypyrrole-carbon Nanotube-Horseradish Peroxidase Composite Film Electrode for the Detection of Phenolic Compounds,” Current Applied Physics, Vol. 11, No. 3, 2011, pp. 402-408. http://dx.doi.org/10.1016/j.cap.2010.08.010
R. Solna and P. Skladal, “Amperometric Flow-Injection Determination of Phenolic Compounds Using a Biosensor with Immobilized Laccase, Peroxidase and Tyrosinase,” Electroanalysis, Vol. 17, No. 23, 2005, pp. 2137-2146. http://dx.doi.org/10.1002/elan.200403343
A. Sassolas, L. J. Blum and B. D. Leca-Bouvier, “Immobilization Strategies to Develop Enzymatic Biosensors,” Biotechnology Advances, Vol. 30, No. 3, 2012, pp. 489511. http://dx.doi.org/10.1016/j.biotechadv.2011.09.003
J. Kulys and R. Vidziunaite, “Amperometric Biosensors Based on Recombinant Laccases for Phenols Determination,” Biosensors and. Bioelectronics, Vol. 18, No. 2-3, 2003, pp. 319-325. http://dx.doi.org/10.1016/S0956-5663(02)00172-0
L.-S. Duan, F. Xie, F. Zhou and S.-F. Wang, “The Electrochemical Behavior of Acetaminophen on Multi-Walled Carbon Nanotubes Modified Electrode and Its Analytical Application,” Analytical Letters, Vol. 40, No. 14, 2007, pp. 2653-2663. http://dx.doi.org/10.1080/00032710701588341
M. A. Heras, S. Lupu, L. Pigani, C. Pirvu, R. Seeber, F. Terzi and C. Zanardi, “A poly(3,4-ethylenedioythiophene)-poly(styrne sulphonate) Composite Electrode Coating in the Electrooxidation of Phenol,” Electrochimica Acta, Vol. 50, No. 7-8, 2005, pp. 1685-1691. http://dx.doi.org/10.1016/j.electacta.2004.10.029
M. Ferreira, H. Varela, R. M. Torresi and G. Tremiliosi-Filho, “Electrode Passivation Caused by Polymerization of Different Phenolic Compounds,” Electrochimica Acta, Vol. 52, No. 2, 2006, pp. 434-442. http://dx.doi.org/10.1016/j.electacta.2006.05.025
T. Spataru and N. Spataru, “Voltammetric Detection of Phenol at Platinum-Polytyramine Composite Electrodes in Acidic Media,” Journal of Hazardous Materials, Vol. 180, No. 1-3, 2010, pp. 777-780. http://dx.doi.org/10.1016/j.jhazmat.2010.04.058
T. A. Enache and A. M. Oliveira-Brett, “Phenol and Para-Substituted Phenols Electrochemical Oxidation Pathways,” Journal of Electroanalytical Chemistry, Vol. 655, No. 1, 2011, pp. 9-16. http://dx.doi.org/10.1016/j.jelechem.2011.02.022
M. Pohanka and P. Skladal, “Electrochemical Biosensors —Principles and Applications”, Journal of Applied Biomedicine, Vol. 6, No. 2, 2008, pp. 57-64.
J. Wang, “Carbon Nanotube Based Electrochemical Biosensors: A Review,” Electroanalysis, Vol. 17, No. 1, 2005, pp. 7-14. http://dx.doi.org/10.1002/elan.200403113
A. S. Santos, A. C. Pereira, M. D. P. T. Sotomayor, C. R. T. Tarley, N. D. Lauro and T. Kubota, “Determination of Phenolic Compounds Based on Co-Immobilization of Methylene Blue and HRP on Multi-Wall Carbon Nanotubes” Electroanalysis, Vol. 19, No. 5, 2007, pp. 549-554. http://dx.doi.org/10.1002/elan.200603759
S. Campuzano, B. Serra, M. Pedrero, F. J. M. Villena and J. M. Pingarrón, “Amperometric Flow-Injection Determination of Phenolic Compounds at Self-Assembled Monolayer-Based Tyrosinase Biosensors,” Analytica Chimica Acta, Vol. 494, No. 1-2, 2003, pp.187-197. http://dx.doi.org/10.1016/S0003-2670(03)00919-X
T. E. Barman, “Enzyme Handbook,” Springer, New York, 1985.
J.-S. Wang, P.-Y. Chen, T.-T. Huang and M.-S. Lin, “Enzymeless Flow Injection Analysis of 2,4,6-Trichlorophenol Based on Preoxidation by Ammonium Cerium (IV) Nitrate,” International Journal of Electrochemical Science, Vol. 7, No. 10, 2012, pp. 9113-9121.
S. K. Ozoner, M. Yalvac and E. Erhan, “Flow Injection Determination of Catechol Based on Polypyrrole-Carbon Nanotube-Tyrosinase Biocomposite Detector,” Current Applied Physics, Vol. 10, No. 1, 2010, pp. 323-328. http://dx.doi.org/10.1016/j.cap.2009.06.017
D. Vega, L. Agui, A. Gonzalez-Cortes, P. Yanez-Sedeno and J. M. Pingarron, “Electrochemical Detection of Phenolic Estrogenic Compounds at Carbon Nanotube-Modified Electrodes,” Talanta, Vol. 71, No. 3, 2007, pp. 1031-1038. http://dx.doi.org/10.1016/j.talanta.2006.05.071
K. Schachl, H. Alemu, K. Kalcher, J. Jezkova, I. Svanvara and K. Vytras, “Amperometric Determination of Hydrogen Peroxide with a Manganese Dioxide-Modified Carbon Paste Electrode Using Flow Injection Analysis,” Analyst, Vol. 122, No. 9, 1997, 985-989. http://dx.doi.org/10.1039/a701723e
A. Remes, A. Pop, F. Manea, A. Baciu, S. J. Picken and J. Schoonman, “Electrochemical Determination of Pentachlorophenol in Water on a Multi-Wall Carbon Nanotubes-Epoxy Composite Electrode,” Sensors, Vol. 12, No. 6, 2012, pp. 7033-7046. http://dx.doi.org/10.3390/s120607033
R. S. Freire, N. Durán and L. T. Kubota, “Electrochemical Biosensor-Based Devices for Continuous Phenols Monitoring in Environmental Matrices,” Journal of Brazilian Chemical Society, Vol. 13, No. 4, 2002, pp. 456-462. http://dx.doi.org/10.1590/S0103-50532002000400008
J. W. Zhao, D. H. Wu and J. F. Zhi, “A Novel Tyrosinase Biosensor Based on Biofunctional ZnO Nanorod Micro-arrays on the Nanocrystalline Diamond Electrode for Detection of Phenolic Compounds,” Bioelectrochemistry, Vol. 75, No. 1, 2009, pp. 44-49. http://dx.doi.org/10.1016/j.bioelechem.2009.01.005