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Rapid Determination of Residual Quinolones in Honey Samples by Fast HPLC with an On-Line Sample Pretreatment System
Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
Shimadzu Corporation, Kyoto, Japan
- 1 Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
- 2 Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
- 3 Shimadzu International Trading (Shanghai) Co., Limited, Beijing, China
- 4 Shimadzu Corporation, Kyoto, Japan
American Journal of Analytical Chemistry·Volume 02 (2011)·Pages 200–205·Published 25 February 2011·DOI10.4236/ajac.2011.22023
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Abstract
A method of on-line pretreatment coupled to HPLC with fluorescence detection was developed and validated for the determination of nine quinolones in honey samples. This method simplified the complicated process of sample pretreatment and reduced sample treatment time. Recovery of the quinolones was between 92% - 101% for spiked honey samples. The limit of detection was 0.22 - 3.78 ng/mL (signal/noise ratio = 3). There was good linear correlation between HPLC peak area and concentration of the quinolones, with a linear range of 1.0 - 100.0 ng/mL and correlation coefficients >0.9997. The method proposed was validated for detecting quinolones in honey samples.
KeywordsAntibioticsHigh Performance Liquid Chromatography (HPLC)HoneyOn-Line PretreatmentQuinolones
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