Research ArticleOpen AccessGoogle Scholar indexed
On the Question of Defining the Association Constants by the Method of Fluorescence Quenching
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation
- 1 Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation
- 2 Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, Novosibirsk, Russian Federation
American Journal of Analytical Chemistry·Volume 05 (2014)·Pages 1065–1068·Published 25 November 2014·DOI10.4236/ajac.2014.516113
Copy link · social · email
Abstract
A study is made on the previously ignored problem of the dependence of a static fluorescence quenching Stern-Volmer constant <i>K</i><sub>sv</sub> on the initial concentration of [F]<sub>0</sub> fluorophore F. This correlation is shown to exist. It is concluded that the Stern-Volmer quenching constant may be used as association constant <i>K</i> only with .
KeywordsFluorescence QuenchingAssociation ConstantsStern-Volmer Constant
- De Weert, M. and Stella, L. (2011) Fluorescence Quenching and Ligand Binding: A Critical Discussion of a Popular Methodology. Journal of Molecular Structure, 998, 144-150. http://dx.doi.org/10.1016/j.molstruc.2011.05.023
- Lakovicz, J. (2010) Principles of Fluorescence Spectroscopy. 3rd Edition, Springer, Berlin.