Direct Colorimetric Detection of Hydrogen Peroxide Using 4-Nitrophenyl Boronic Acid or Its Pinacol Ester
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Abstract
A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to at pH 11 and 80?C. The linear range for the colorimetric method extends beyond 1.0 to 40 µM H2O2, and the limit of detection is ~1.0 µM H2O2. This method offers a convenient and practical process for rapid determination of hydrogen peroxide in aqueous media. Compared to many other techniques in H2O2 detection, this process is a direct measurement of H2O2, and is relatively unaffected by the presence of various salts, metal ions and the chelator EDTA.
- Y. Zuo and J. Hoigné, “Evidence for Photochemical Formation of H2O2 and Oxidation of SO2 in Authentic Fog Water,” Science, Vol. 260, No. 5104, 1992, pp. 71- 73. doi:10.1126/science.260.5104.71
- A. Tahirovic, A. Copra, E. Omanovic-Miklicanin and K. Kalcher, “A Chemiluminescence Sensor for the Deter- mination of Hydrogen Peroxide,” Talanta, Vol. 72, No. 4, 2007, pp. 1378-1385. doi:10.1016/j.talanta.2007.01.072
- G. Georgiou and L. Masip, “An Overoxidation Journey with a Return Ticket,” Science, Vol. 300, No. 5619, 2003, pp. 592-594. doi:10.1126/science.1084976
- A. Navas Diaz, F. G. Sanchez, M. C. Torijas and J. Lovillo, “Chemiluminescent Lipase Determination Based on the Enhanced Luminol/H2O2/horsedadish Peroxidase/ Fluorescein Diacetate Energy Transfter System,” Fresenius’ Journal of Analytical Chemistry, Vol. 365, 1999, pp. 537-540. doi:10.1007/s002160051518
- H. J. H. Fenton, “Oxidation of Tartaric Acid in Presence of Iron,” Journal of the Chemical Society, Vol. 65, 1894, pp. 899-910. doi:10.1039/ct8946500899
- B. Halford, “Explosives Detection: Sensor Capitalizes on Contrasts in Redox Chemistry,” Chemical & Engineering News, Vol. 86, No. 11, 2008, p. 10. doi:10.1021/cen-v086n011.p010
- F. Sauer, S. Limbach and G. K. Moortgat, “Measure- ments of Hydrogen Peroxide and Individual Organic Peroxides in the Marine Troposphere,” Atmospheric En- vironment, Vol. 31, No. 8, 1997, pp. 1173-1184. doi:10.1016/S1352-2310(96)00289-0
- J. Tang, B. Wang, Z. Wu, X. Han, S. Dong and E. Wang, “Lipid Membrane Immobilized Jorseradish Peroxidase Biosensor for Amperometric Determination of Hydrogen Peroxide,” Biosensors & Bioelectronics, Vol. 18, No. 7, 2003, pp. 867-872. doi:10.1016/S0956-5663(02)00148-3
- C. Matsubara, K. Kudo, T. Kawashita and K. Takamura, “Spectrophotometric Determination of Hydrogen Perox- ide with Titanium 2-((5-Bromopyridyl)azo)-5-(N-Pro- pyl-N-Sulfopropylamino)Phenol Reagent and Its Application to the Determination of Serum Glucose Using Glu- cose Oxidase,” Analytical Chemistry, Vol. 57, No. 6, 1985, pp. 1107-1109. doi:10.1021/ac00283a032
- S. Wolff, “Ferrous Ion Oxidation in Presence of Ferric Ion Indicator Xylenol Orange for Measurement of Hy- droperoxides,” Methods in Enzymology, Vol. 233, 1994, pp. 182-189. doi:10.1016/S0076-6879(94)33021-2
- P. A. Tanner and A. Y. S. Wong, “Spectrophotometric Determination of Hydrogen Peroxide in Rainwater,” Ana- lytica Chimica Acta, Vol. 370, No. 2-3, 1998, pp. 279- 287. doi:10.1016/S0003-2670(98)00273-6